Genotypic characterization of Echinococcus granulosus in Iranian goats

Youssefi, Mohammad Reza; Tabaripour, Rabeeh; Omrani, Vahid Fallah; Spotin, Adel; Esfandiari, Behzad

doi:10.1016/s2222-1808(13)60085-7

Cited by 20 publications

(14 citation statements)

References 35 publications

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“…and morphological aspects (21,28). However, few studies have been carried out on the identification of Echinococcus spp.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have attempted to genotype hydatid cysts in human, sheep, goat, cattle, buffalo, and camel populations in Iran on the basis of mitochondrial (cox1 and nad1) and nucleus markers, using RAPD-PCR, PCR-RFLP and sequencing strategies (19,20,22,28,30,36,37). However, none of them could provide remarkable applications in mixed genotypes.…”

Section: Discussionmentioning

confidence: 99%

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Discrimination of Mixed Infections of Echinococcus Species Based on in Silico Sequence Analysis: A New Way of Reflecting Overlapped Strains in Indigenous Areas

Karimi

Ghasemikhah²,

Spotin

et al. 2017

Arch Clin Infect Dis

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Objectives: Hydatidosis, as a cyclo-zoonotic indigenous disease, is marked by various strains and multiple infections around the world where different overlapping cycles of Echinococcus spp. are explicitly circulating among intermediate and definitive hosts. One of the current challenges in Echinococcus taxonomy is associated with the imprecise identification of dual infections in endemic regions. The aim of this study was to genotype Echinococcus strains, particularly those ignored during mixed infections, by employing phylogenetic analysis in order to determine the accurate status of the parasite in endemic areas. Methods: 60 isolates were collected from the liver and lungs of twenty humans, fifteen sheep, fifteen goat, and ten cattle during 2012 -14 in Central Iran. Subsequently, the extraction of DNA was performed, followed by amplification and identification of the DNA through both PCR for internal transcribed spacer 1 (ITS1-rDNA) of ribosomal DNA and restriction fragment length polymorphism (RFLP) using two endonuclease enzymes: Rsa1 and HpaII. Then, the isolates were directly sequenced in order to accomplish the definite identification of strains/haplotypes. In addition, the mixed infections were analyzed by mixed sequences reader (MSA). Results: The RFLP, phylogenetic analyses of ITS1-rDNA sequences and MSA finding based on analyzed chromatograms strongly revealed the sheep strain (n = 58) and two mixed infection G1 and G6 genotypes (n = 2). The five specific haplotypes that represent moderate diversity indices were identified. The haplotype diversity was estimated to be 0.522, with a nucleotide diversity of 0.02. Conclusions: The G1 was reported to be the dominant strain in the central regions of Iran; however, the first dual discrimination of G1 and G6 genotypes in these regions showed that a sheep-camel/dog life cycle is circulating in central Iran. The current findings can facilitate the parasite taxonomic classification based on in silico analysis particularly about those that are neglected in dual infections. Additional studies are highly needed with a greater sample size and in various regions of Iran.

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“…and morphological aspects (21,28). However, few studies have been carried out on the identification of Echinococcus spp.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Discrimination of Mixed Infections of Echinococcus Species Based on in Silico Sequence Analysis: A New Way of Reflecting Overlapped Strains in Indigenous Areas

Karimi

Ghasemikhah²,

Spotin

et al. 2017

Arch Clin Infect Dis

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“…Dogs and humans act as definitive and intermediate hosts, respectively. Human contamination occurs via poorly washed vegetables contaminated with the ova of E. granulosus (2,3). When the visceral organs of intermediate hosts are eaten by canines, the cysts transform to adult worms in their small intestine (2).…”

Section: Introductionmentioning

confidence: 99%

“…Human contamination occurs via poorly washed vegetables contaminated with the ova of E. granulosus (2,3). When the visceral organs of intermediate hosts are eaten by canines, the cysts transform to adult worms in their small intestine (2). Hydatid cyst is frequently localized in the liver (60% -70%) and lungs (10% -15%) (4).…”

Section: Introductionmentioning

confidence: 99%

“…Hydatid cyst is frequently localized in the liver (60% -70%) and lungs (10% -15%) (4). However, the cases of the central nervous system (brain, spinal cord, and orbit) are responsible for 2% to 3% of the reported hydatid cysts (2,5). The intraorbital cyst is very uncommon, reported being less than 1% of all hydatid diseases in endemic areas (2,4).…”

Section: Introductionmentioning

confidence: 99%

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A Rare Case Series of Intraorbital Unilocular Hydatid Cysts in Pediatric Patients

Nabie

Halimi

Spotin

2017

Arch Clin Infect Dis

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Introduction: The orbital hydatidosis due to Echinococcus granulosus accounts for only less than 1% of all hydatid cysts. Here we report a case series of intraorbital hydatid cysts. Case Presentation: This retrospective investigation included two pediatric patients (4 and 13-year-old boys) who were referred because of a progressive proptosis in northwest Iran. Orbital imaging disclosed cystic intraorbital lesions in the cases. Following clinical and surgical explorations, the histopathological evaluation re-confirmed definitely the infertile unilocular hydatid cysts. Conclusions: Differential diagnoses of orbital hydatid cysts from cystic lesions should be explicitly noticed in endemic areas prior to surgery in order to avoid complications.

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Designing and conducting in silico analysis for identifying of Echinococcus spp. with discrimination of novel haplotypes: an approach to better understanding of parasite taxonomic

et al. 2015

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The definitive identification of Echinococcus species is currently carried out by sequencing and phylogenetic strategies. However, the application of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) patterns is not broadly used as a result of heterogeneity traits of Echinococcus genome in different regions of the world. Therefore, designing and conducting a standardized pattern should indigenously be considered in under-studied areas. In this investigation, an in silico mapping was designed and developed for eight Echinococcus spp. on the basis of regional sequences in Iran and the world. The numbers of 60 Echinococcus isolates were collected from the liver and lungs of 15 human, 15 sheep, 15 cattle, and 15 camel cases in Semnan province, Central Iran. DNA samples were extracted and examined by polymerase chain reaction of ribosomal DNA (rDNA) internal transcribed spacer 1 (ITS1) and PCR-RFLP via Rsa1 endonuclease enzyme. Moreover, 15 amplicons of cytochrome oxidase 1 (Cox1) were directly sequenced in order to identify the strains/haplotypes. PCR-RFLP and phylogenetic analyses revealed firmly the presence of the G1 and G6 genotypes with heterogeneity (three novel haplotypes) of Cox1 gene although no other expected genotypes were found in the region. Finding shows that the identification of novel haplotypes along with discrimination of Echinococcus spp. through regional patterns can unambiguously illustrate the real taxonomic status of parasite in Central Iran.

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Genotypic characterization of Echinococcus granulosus in Iranian goats

Cited by 20 publications

References 35 publications

Discrimination of Mixed Infections of Echinococcus Species Based on in Silico Sequence Analysis: A New Way of Reflecting Overlapped Strains in Indigenous Areas

Discrimination of Mixed Infections of Echinococcus Species Based on in Silico Sequence Analysis: A New Way of Reflecting Overlapped Strains in Indigenous Areas

A Rare Case Series of Intraorbital Unilocular Hydatid Cysts in Pediatric Patients

Designing and conducting in silico analysis for identifying of Echinococcus spp. with discrimination of novel haplotypes: an approach to better understanding of parasite taxonomic

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