Genotypic characterization and phylogenetic analysis of Cryptosporidium sp. from domestic animals in Brazil

Huber, Franziska; Silva, S. da; Bomfim, Teresa Cristina Bergamo do; Teixeira, K. R. dos S.; Bello, Alexandre Ribeiro

doi:10.1016/j.vetpar.2007.08.018

Cited by 78 publications

(68 citation statements)

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“…Among the species/genotypes, C. baileyi was originally isolated from commercial broiler chickens and is probably the most common avian Cryptosporidium species. In addition to the chickens and ducks, it has been reported in more than 17 other avian hosts (Huber et al, 2007;Amer et al, 2010;Ryan, 2010). C. meleagridis also appears to have a broad host range and has been found in various avian species including chickens, turkeys, parrots, and cockatiels (O'Donoghue, 1995;Morgan et al, 2000;Sreter & Varga, 2000;Darabus & Olariu, 2003;Abe & Iseki, 2004;Huber et al, 2007).…”

Section: Methodsmentioning

confidence: 99%

“…However, most reports on the prevalence in chickens were published in the 1980s and 1990s, with infection rates ranging from 4.5 to 50% depending on the location and the methods used in detection (de Graaf et al, 1999). In contrast, in the past 10 years there have been only about six reports (Trampel et al, 2000;Kimura et al, 2004;Huber et al, 2007;Soltane et al, 2007;Shemshadi et al, 2010;Smith et al, 2010). Likewise, there are only a few reports (about seven) concerning Cryptosporidium infections in ducks (Mason, 1986;Richter et al, 1994;O'Donoghue, 1995;Morgan et al, 2001;Kuhn et al, 2002;Huber et al, 2007;Amer et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Large-scale survey ofCryptosporidiumspp. in chickens and Pekin ducks (Anas platyrhynchos) in Henan, China: prevalence and molecular characterization

et al. 2010

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Few data are available on the molecular characterization of Cryptosporidium spp. in chickens and ducks in China. In this study, 2579 faecal samples from 46 chicken farms and eight Pekin duck farms in 21 prefectures in Henan Province were examined. The overall infection rate of Cryptosporidium was 10.6% (163/1542) in layer chickens (10 out of 17 farms), 3.4% (16/473) in broilers (five out of 29 farms), and 16.3% (92/564) in Pekin ducks (four out of eight farms), respectively. The highest infection rates were observed in 31-day-old to 60-day-old layer chickens (24.6%) and 11-day-old to 30-day-old Pekin ducks (40.3%). The season of highest prevalence in chickens was spring (15.6%) and the lowest was winter (P B0.01). One hundred and eightyseven Cryptosporidium-positive samples were analysed by polymerase chain reaction (PCR)Árestriction fragment length polymorphism analysis of the small subunit rRNA gene, and 55 were further analysed by DNA sequencing of the PCR products. Two Cryptosporidium species were identified: Cryptosporidium baileyi (184/187) on 15 chicken farms and four duck farms, and Cryptosporidium meleagridis (3/187) on three layer chicken farms. C. baileyi was the predominant Cryptosporidium species, found in all age groups of chickens and all Cryptosporidium-positive ducks examined, whereas C. meleagridis was only identified in 31-day-old to 120-day-old layer chickens. Considering the large size of the chicken industry and the close contact between chickens and humans, and that C. meleagridis is the third most common Cryptosporidium parasite in humans, then C. meleagridis could potentially become an emerging zoonosis in some areas in China.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Large-scale survey ofCryptosporidiumspp. in chickens and Pekin ducks (Anas platyrhynchos) in Henan, China: prevalence and molecular characterization

et al. 2010

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“…Like C. parvum, C. meleagridis appears to have a large host range and has been described from a wide range of avian species including turkeys, parrots, chickens, cockatiels and a red-legged partridge (O'Donoghue, 1995, Morgan, et al, 2000b, Darabus and Olariu, 2003, Abe and Iseki, 2004Huber et al, 2007;Pagès-Manté et al, 2007;Soltane et al, 2007). Experimental transmission studies have shown that C. meleagridis can infect broiler chickens, ducks, turkeys, calves, pigs, rabbits, rats, mice (O'Donoghue, 1995, Darabus and Olariu, 2003, Huang, et al, 2003.…”

Section: Cryptosporidium Meleagridis Slavin 1955mentioning

confidence: 99%

“…Cryptosporidium baileyi is probably the most common avian Cryptosporidium sp., and has been reported in a wide range of avian hosts including black-headed gulls, chickens, cormorants, cranes, a channel-billed toucan, an eastern golden-backed weaver, turkeys, ducks, geese, cockatiels, a brown quail, a grey-bellied bulbul, an ostrich, a red-rumped cacique, a crested oropendola, a red crowned amazon, a rose-ringed parakeet, a grey partridge and mixed-bred falcons , Pavlásek, 1993, Ryan, et al, 2003a, Abe and Iseki, 2004, Jellison, et al, 2004, Kimura et al, 2004, Chvala, et al, 2006Huber et al, 2007;Van Zeeland et al, 2008).…”

Section: Cryptosporidium Baileyi Current Upton and Haynes 1986mentioning

confidence: 99%

Cryptosporidium in birds, fish and amphibians

Ryan

2010

Experimental Parasitology

131

110

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“…No laboratório de Protozoologia do Departamento de Parasitologia, da Universidade Federal Rural do Rio de Janeiro, as amostras foram submetidas a técnica de centrifugação e flutuação com solução saturada de açúcar, de acordo com a metodologia descrita por Huber et al, 2007. As lâminas foram preparadas e observadas com objetiva de 40X. Naquelas amostras positivas para a presença de oocistos de Cryptosporidium spp.…”

Section: Methodsunclassified

Infecção natural de pombos (Columba livia) por Cryptosporidium spp

Costa¹,

Bomfim²

2016

RBCV

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ResumoO estudo teve como objetivo diagnosticar microscopicamente e molecularmente a presença de oocistos de Cryptosporidium spp. provenientes de amostras fecais de pombos (Columba livia) no município do Rio de Janeiro. As amostras fecais foram coletadas frescas, logo após as aves defecarem e acondicionadas sob refrigeração e encaminhadas para o processamento e pesquisa de oocistos de Cryptosporidium spp. ao laboratório de Protozoologia do Departamento de Parasitologia, da Universidade Federal Rural do Rio de Janeiro. As amostras foram submetidas a técnica de centrifugação e flutuação com solução saturada de açúcar e as lâminas observadas com objetiva de 40X. Nas amostras positivas para a presença de oocistos de Cryptosporidium spp. foi realizada a extração de ácido desoxirribonucleico (DNA) para uso nas reações em cadeia pela polimerase (PCR) e Nested-PCR. Um total de 387 amostras fecais de pombos foram obtidas, no diagnóstico microscópico foi possível a observação de oocistos deste parasita em 81 amostras (20,93%). Destas, 53 amostras amplificaram DNA específico para Cryptosporidium spp. na reação da Nested-PCR. Mundialmente, Cryptosporidium spp. parasitando pombos é assinalado em apenas quatro países: Turquia, China, Irã e Tailândia. O resultado encontrado neste trabalho, em três bairros populosos do município do Rio de Janeiro, revelou ser preocupante, por serem áreas onde há uma quantidade acentuada da população animal e humana, facilitando a dispersão e contaminação ambiental dos oocistos. Este é o primeiro registro no Brasil de parasitismo de Cryptosporidium spp. tendo como hospedeiro o pombo e estes resultados, não devem ser negligenciados.Palavras-chave: Columbidae, Rio de Janeiro, Criptosporidiidae, diagnóstico microscópico, diagnóstico molecular. AbstractThe aim of this study was to diagnose microscopically and molecularly the presence of Cryptosporidium spp. oocysts from pigeons (Columba livia) faecal samples in Rio de Janeiro city. The fecal samples were collected fresh, after the birds defecated and conditioned under refrigeration and sent to the processing and research of Cryptosporidium spp. oocysts to the protozoology laboratory of the Parasitology Department, Universidade Federal Rural do Rio de Janeiro. The samples were submitted to centrifugation and flotation with saturated sugar solution technique and the slides observed with a 40X objective. In the positive samples for the Cryptosporidium spp. oocysts the extraction of deoxyribonucleic acid (DNA) for polymerase chain reaction (PCR) and Nested-PCR was performed. A total of 387 pigeons faecal samples were obtained, in the microscopic diagnosis it was possible to observe oocysts of this parasite in 81 samples (20.93%). Of these, 53 samples amplified DNA specific for Cryptosporidium spp. in the Nested-PCR reaction. Worldwide, Cryptosporidium spp. parasitizing pigeons is reported in only four countries: Turkey, China, Iran and Thailand. The results found in this study, in three populated neighborhoods of Rio de Janeiro city, were worrisome becau...

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Genotypic characterization and phylogenetic analysis of Cryptosporidium sp. from domestic animals in Brazil

Cited by 78 publications

References 36 publications

Large-scale survey ofCryptosporidiumspp. in chickens and Pekin ducks (Anas platyrhynchos) in Henan, China: prevalence and molecular characterization

Large-scale survey ofCryptosporidiumspp. in chickens and Pekin ducks (Anas platyrhynchos) in Henan, China: prevalence and molecular characterization

Cryptosporidium in birds, fish and amphibians

Infecção natural de pombos (Columba livia) por Cryptosporidium spp

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