Genotypic and phenotypic identification of Aeromonas species and CphA-mediated carbapenem resistance in Queensland, Australia

Sinclair, Holly A.; Heney, Claire; Sidjabat, Hanna E.; George, Narelle; Bergh, Haakon; Anuj, S.N.; Nimmo, Graeme R.; Paterson, David L.

doi:10.1016/j.diagmicrobio.2016.02.005

Cited by 53 publications

(55 citation statements)

References 34 publications

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“…; Sinclair et al . ) . A. hydrophila is the most frequently reported Aeromonas species causing alligator or crocodile skin lesions and septicaemia (Turutoglu et al .…”

Section: Resultsmentioning

confidence: 97%

“…At the species level, A. dhakensis, A. hydrophila and A. jandaei were identified by biochemical and molecular examination, although it remains to be determined which species was the cause of primary or secondary infection; A. dhakensis exhibited the greatest virulence in the zebrafish experiment. Until this study, the opportunistic pathogens A. dhakensis and A. jandaei had only been isolated from diseased fish and humans(Deng et al 2016;Sinclair et al 2016). A. hydrophila is the most frequently reported Aeromonas species causing alligator or crocodile skin lesions and septicaemia(Turutoglu et al 2005;Roh et al 2011) but has not previously been isolated from Siamese crocodile.…”

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confidence: 94%

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Three species ofAeromonas(A. dhakensis, A. hydrophila and A. jandaei) isolated from freshwater crocodiles (Crocodylus siamensis) with pneumonia and septicemia

Guo

Yang

et al. 2019

Lett Appl Microbiol

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Hundreds of farmed Siamese crocodiles (Crocodylus siamensis) died during July 2016 at a farm in Wenchang, Hainan, China. In two necropsied crocodiles, we observed symptoms of dermatorrhagia, hepatomegaly and hepatic congestion. Pulmonitis was diagnosed by pulmonary congestion and pulmonary fibrinous exudate. Septicaemia was diagnosed by isolation of three Aeromonas species from blood and visceral tissues; A. dhakensis, A. hydrophila and A. jandaei were identified by biochemical and molecular tests. We used a zebrafish model to determine the half‐maximal lethal dose (LD50), and A. dhakensis was found to be the most virulent species, with an LD50 of 8·91 × 105 CFU per ml. The results of a drug sensitivity test indicated that these species were sensitive to 11 antibiotics. This is the first report of A. dhakensis, A. hydrophila and A. jandaei being isolated from a mixed infection in Siamese crocodiles. Significance and Impact of the Study In this study, we isolated three species of Aeromonas (A. dhakensis, A. hydrophila and A. jandae) from farmed Siamese crocodiles with fatal fibrinous pneumonia and septicaemia. This is the first description of a mixed infection with three Aeromonas species among captive crocodilians.

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“…; Sinclair et al . ) . A. hydrophila is the most frequently reported Aeromonas species causing alligator or crocodile skin lesions and septicaemia (Turutoglu et al .…”

Section: Resultsmentioning

confidence: 97%

mentioning

confidence: 94%

Three species ofAeromonas(A. dhakensis, A. hydrophila and A. jandaei) isolated from freshwater crocodiles (Crocodylus siamensis) with pneumonia and septicemia

Guo

Yang

et al. 2019

Lett Appl Microbiol

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“…CphA, a MBL found in A. dhakensis, A. veronii , and A. hydrophila ( Wu et al, 2012a ), has a specific substrate profile of hydrolyzing carbapenems but not penicillins or cephalosporins, if compared with other MBLs ( Segatore et al, 1993 ). However, CphA carbapenemase production is not easily detected by the conventional susceptibility test, unless a large inoculum or additional tests (e.g., modified Hodge test, ertapenem MIC or Carba NP test) are applied ( Rossolini et al, 1995 ; Wu et al, 2012a ; Sinclair et al, 2016 ). Carbapenem therapy for cphA- carrying Aeromonas infections remains controversial, because of the clinical concern that carbapenem monotherapy may fail in treating MBL-producing aeromonads infections with a high bacterial burden, such as peritonitis/abdominal sepsis or soft tissue infections ( Wu et al, 2012a ).…”

Section: Antimicrobial Resistance and Therapymentioning

confidence: 99%

Aeromonas dhakensis, an Increasingly Recognized Human Pathogen

2016

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Aeromonas dhakensis was first isolated from children with diarrhea in Dhaka, Bangladesh and described in 2002. In the past decade, increasing evidence indicate this species is widely distributed in the environment and can cause a variety of infections both in human and animals, especially in coastal areas. A. dhakensis is often misidentified as A. hydrophila, A. veronii, or A. caviae by commercial phenotypic tests in the clinical laboratory. Correct identification relies on molecular methods. Increasingly used matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) may be able to identify Aeromonas specie rapidly and accurately. A. dhakensis has shown its potent virulence in different animal models and clinical infections. Although several virulence factors had been reported, no single mechanism is conclusive. Characteristically A. dhakensis is the principal species causing soft tissue infection and bacteremia, especially among patients with liver cirrhosis or malignancy. Of note, A. dhakensis bacteremia is more lethal than bacteremia due to other Aeromonas species. The role of this species in gastroenteritis remains controversial. Third generation cephalosporins and carbapenems should be used cautiously in the treatment of severe A. dhakensis infection due to the presence of AmpC ββ-lactamase and metallo-β-lactamase genes, and optimal regimens may be cefepime or fluoroquinolones. Studies of bacterial virulence factors and associated host responses may provide the chance to understand the heterogeneous virulence between species. The hypothesis A. dhakensis with varied geographic prevalence and enhanced virulence that compared to other Aeromonas species warrants more investigations.

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“…play a major role in the transfer of antibiotic resistance, making these organisms particularly problematic. They have been implicated as mediators of the transfer of antibiotic resistance markers between hospital and environmental strains (Varela et al, 2016), and are associated with innate multi-antibiotic resistance due to efflux pumps, inducible cephalosporinases, and inducible metallo beta lactamases (Azzopardi et al, 2011;Sinclair et al, 2016). Controlling A. hydrophila infection is therefore paramount as this organism threatens food security (by causing fish diseases and increasing their mortality) (Talagrand-Reboul et al, 2017) and human health (Neyts et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Novel Bacteriophages Capable of Disrupting Biofilms From Clinical Strains of Aeromonas hydrophila

et al. 2020

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The increase in global warming has favored growth of a range of opportunistic environmental bacteria and allowed some of these to become more pathogenic to humans. Aeromonas hydrophila is one such organism. Surviving in moist conditions in temperate climates, these bacteria have been associated with a range of diseases in humans, and in systemic infections can cause mortality in up to 46% of cases. Their capacity to form biofilms, carry antibiotic resistance mechanisms, and survive disinfection, has meant that they are not easily treated with traditional methods. Bacteriophage offer a possible alternative approach for controlling their growth. This study is the first to report the isolation and characterization of bacteriophages lytic against clinical strains of A. hydrophila which carry intrinsic antibiotic resistance genes. Functionally, these novel bacteriophages were shown to be capable of disrupting biofilms caused by clinical isolates of A. hydrophila. The potential exists for these to be tested in clinical and environmental settings.

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Genotypic and phenotypic identification of Aeromonas species and CphA-mediated carbapenem resistance in Queensland, Australia

Cited by 53 publications

References 34 publications

Three species ofAeromonas(A. dhakensis, A. hydrophila and A. jandaei) isolated from freshwater crocodiles (Crocodylus siamensis) with pneumonia and septicemia

Three species ofAeromonas(A. dhakensis, A. hydrophila and A. jandaei) isolated from freshwater crocodiles (Crocodylus siamensis) with pneumonia and septicemia

Aeromonas dhakensis, an Increasingly Recognized Human Pathogen

Novel Bacteriophages Capable of Disrupting Biofilms From Clinical Strains of Aeromonas hydrophila

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