Genotypic and phenotypic characterization of Bordetella pertussis strains used in different vaccine formulations in Latin America

Abstract

Aim: To characterize Bordetella pertussis vaccine strains in comparison with current circulating bacteria. Methods and Results: Genomic and proteomic analyses of Bp137 were performed in comparison with other vaccine strains used in Latin America (Bp509 and Bp10536) and with the clinical Argentinean isolate Bp106. Tohama I strain was used as reference strain. Pulse‐field gel electrophoresis (PFGE) and pertussis toxin promoter (ptxP) sequence analysis revealed that Bp137 groups with Bp509 in PFGE group III and contains ptxP2 sequence. Tohama I (group II) and Bp10536 (group I) contain ptxP1 sequence, while Bp106 belongs to a different PFGE cluster and contains ptxP3. Surface protein profiles diverged in at least 24 peptide subunits among the studied strains. From these 24 differential proteins, Bp10536 shared the expression of ten proteins with Tohama I and Bp509, but only three with Bp137. In contrast, seven proteins were detected exclusively in Bp137 and Bp106. Conclusions: Bp137 showed more features in common with the clinical isolate Bp106 than the other vaccine strains here included. Significance and Impact of the Study: The results presented show that the old strains included in vaccines are not all equal among them. These findings together with the data of circulating bacteria should be taken into account to select the best vaccine to be included in a national immunization programme.

Increase in pertussis cases along with high prevalence of two emerging genotypes of Bordetella pertussis in Perú, 2012

“…3 ), with ptxP 3- ptxA 1- prn 2- fim 3-1 being the more prevalent genotype—it having been designated by other authors as MT27a [ 40 ]. The results detected for fim alleles were similar to those reported for the Argentine isolates fim 2-1 (97 % of those studied), and fim3-2 (76 %) [ 33 ].…”

“…DNA sequencing of B. pertussis genomic regions ( ptx P, ptx A, fim 2, fim , and prn ) was performed with the primers listed in the Table 1 , and according to the protocols of reference [ 33 ]. The PCR products in an agarose gel were purified with QIAquick Gel Extraction Kit (QIAGEN) and the sequencing reaction carried out with the BigDye Terminator v. 3.1 (Applied Biosystems) precipitated and resuspended in Hi-Di formamide (Life Technologies).…”

Differentially Expressed Genes in Bordetella pertussis Strains Belonging to a Lineage Which Recently Spread Globally

“…Within a timeframe of 10 years, ptxP3 strains have nearly completely replaced the previously dominant ptxP1 strains. Significantly, ptxP3 strains have spread globally, as they have emerged in several countries in Europe [13]–[17], North America [18], South America [19], and Australia [20]. Phylogenetic analyses showed that ptxP3 strains evolved from ptxP1 strains, possibly in the 1970s [21], [22].…”

Prevalence and Molecular Characterization of Pertactin-Deficient Bordetella pertussis in the United States

“…The genomes of the currently circulating Bordetella pertussis isolates differ from the isolates used in the manufacture of vaccine components (8)(9)(10)(11)(12). Allelic variations in several of the acellular vaccine components, such as ptxP3, prn2, and fim3B, have appeared, and the predominant profile observed no longer completely matches the strains used to make the acellular vaccine components (12)(13)(14)(15).…”