Genotypic analysis of uropathogenic Escherichia coli to understand factors that impact the prevalence of β-lactam-resistant urinary tract infections in a community

Tarlton, Nicole J.; Moritz, C.; Adams‐Sapper, Sheila; Riley, Lee W.

doi:10.1016/j.jgar.2019.03.002

Cited by 12 publications

(10 citation statements)

References 32 publications

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“…Community sources for the OXA-244-producing E. coli isolates seem possible since many of the patients had not been hospitalised and the E. coli isolates belonged to STs that are present in the community [23][24][25][26][27][28][29][30][31][32]. The ECDC RRA identified one main geographically dispersed ST38 OXA-244-producing E. coli cluster with chromosomal-encoded bla OXA-244 present in all 10 countries, including Denmark, that submitted data for the RRA [10].…”

Section: Discussionmentioning

confidence: 99%

Surveillance of OXA-244-producing Escherichia coli and epidemiologic investigation of cases, Denmark, January 2016 to August 2019

et al. 2020

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Background: Carbapenemase-producing Escherichia coli are increasing worldwide. In recent years, an increase in OXA-244-producing E. coli isolates has been seen in the national surveillance of carbapenemase-producing organisms in Denmark. Aim: Molecular characterisation and epidemiological investigation of OXA-244-producing E. coli isolates from January 2016 to August 2019. Methods: For the epidemiological investigation, data from the Danish National Patient Registry and the Danish register of civil registration were used together with data from phone interviews with patients. Isolates were characterised by analysing whole genome sequences for resistance genes, MLST and core genome MLST (cgMLST). Results: In total, 24 OXA-244-producing E. coli isolates were obtained from 23 patients. Among the 23 patients, 13 reported travelling before detection of the E. coli isolates, with seven having visited countries in Northern Africa. Fifteen isolates also carried an extended-spectrum beta-lactamase gene and one had a plasmid-encoded AmpC gene. The most common detected sequence type (ST) was ST38, followed by ST69, ST167, ST10, ST361 and ST3268. Three clonal clusters were detected by cgMLST, but none of these clusters seemed to reflect nosocomial transmission in Denmark. Conclusion: Import of OXA-244 E. coli isolates from travelling abroad seems likely for the majority of cases. Community sources were also possible, as many of the patients had no history of hospitalisation and many of the E. coli isolates belonged to STs that are present in the community. It was not possible to point at a single country or a community source as risk factor for acquiring OXA-244-producing E. coli.

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Section: Discussionmentioning

confidence: 99%

Surveillance of OXA-244-producing Escherichia coli and epidemiologic investigation of cases, Denmark, January 2016 to August 2019

et al. 2020

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“…Of the b-lactamase genes examined, bla CTX-M was the most prevalent, found in 8 (23%) isolates, followed by bla SHV , detected in 5 (15%) isolates, and bla TEM detected in 4 (12%) isolates. The high frequency of E. coli strains carrying bla CTX-M is surprising, considering that the most common b-lactam genes found in clinical strains of E. coli is bla TEM and/or bla SHV (Yamaji et al, 2018;Borges et al, 2019;Tarlton et al, 2019). The high frequency of CTX-M blactamase genes in ready-to-eat lettuce poses a public health concern, since it is the most common extended-spectrum betalactamases (ESBL) expressed by clinical strains of E. coli, which is becoming increasingly prevalent in extraintestinal infections worldwide (Yamaji et al, 2018;Borges et al, 2019;Richter et al, 2019;Tarlton et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

Prevalence of β-Lactam Drug-Resistance Genes in Escherichia coli Contaminating Ready-to-Eat Lettuce

Liao

Borges

Rubin

et al. 2020

Foodborne Pathogens and Disease

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Thirty-four Escherichia coli isolates from 91 ready-to-eat lettuce packages, obtained from local supermarkets in Northern California, were genotyped by multilocus sequence typing, tested for susceptibility to antimicrobial agents, and screened for b-lactamase genes. We found 15 distinct sequence types (STs). Six of these genotypes (ST1198, ST2625, ST2432, ST2819, ST4600, and ST5143) have been reported as pathogens found in human samples. Twenty-six (76%) E. coli isolates were resistant to ampicillin, 17 (50%) to ampicillin/sulbactam, 8 (23%) to cefoxitin, and 7 (20%) to cefuroxime. bla CTX-M was the most prevalent b-lactamase gene, identified in eight (23%) isolates. We identified a class A broad-spectrum b-lactamase SED-1 gene, bla SED , reported by others in Citrobacter sedlakii isolated from bile of a patient. This study found that fresh lettuce carries b-lactam drug-resistant E. coli, which might serve as a reservoir for drug-resistance genes that could potentially be transmitted to pathogens that cause human infections.

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“…xtraintestinal pathogenic Escherichia coli (ExPEC) belonging to multilocus sequence type 420 (ST420) is often reported as a low-prevalence genotype (1,2), but it has been identified sporadically from patients with community-acquired urinary tract infection (UTI) at a college campus in northern California (3)(4)(5). Interestingly, all of those ST420 isolates were found to be susceptible to all antimicrobial agents tested (pansusceptible) (4,5). Here, we present the genome sequence of an ST420 ExPEC strain isolated from one of the patients (a 39-year-old female) diagnosed with UTI at the university health center in 2003 (4).…”

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confidence: 99%

“…Interestingly, all of those ST420 isolates were found to be susceptible to all antimicrobial agents tested (pansusceptible) (4,5). Here, we present the genome sequence of an ST420 ExPEC strain isolated from one of the patients (a 39-year-old female) diagnosed with UTI at the university health center in 2003 (4). In this paper and in GenBank, this ST420 isolate is designated strain IT0021.…”

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confidence: 99%

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Draft Genome Sequence of an Escherichia coli Sequence Type 420 Isolate from a Patient with Urinary Tract Infection in Northern California

Matsui

Tarlton

et al. 2020

Microbiol Resour Announc

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Genotypic analysis of uropathogenic Escherichia coli to understand factors that impact the prevalence of β-lactam-resistant urinary tract infections in a community

Cited by 12 publications

References 32 publications

Surveillance of OXA-244-producing Escherichia coli and epidemiologic investigation of cases, Denmark, January 2016 to August 2019

Surveillance of OXA-244-producing Escherichia coli and epidemiologic investigation of cases, Denmark, January 2016 to August 2019

Prevalence of β-Lactam Drug-Resistance Genes in Escherichia coli Contaminating Ready-to-Eat Lettuce

Draft Genome Sequence of an Escherichia coli Sequence Type 420 Isolate from a Patient with Urinary Tract Infection in Northern California

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