Genotypic Analyses of the Sclerostin rs851056 and Dickkopf rs1569198 Polymorphisms in Mexican-Mestizo Postmenopausal Osteoporosis: A Case–Control Study

Vazquez-Villegas, Maria Luisa; Rodriguez-Jimenez, Norma Alejandra; Contreras-Haro, Betsabe; Vásquez-Jiménez, José Clemente; Pérez-Guerrero, Edsaúl Emilio; Morán-Moguel, María Cristina; Sánchez-Rodríguez, Esther Nérida; Villagómez-Vega, Alejandra; Nuño‐Arana, Ismael; Becerra-Alvarado, Itzel Nayar; Arellano, Edy David Rubio; Nava-Valdivia, César Arturo; Ponce-Guarneros, Juan M; Fajardo-Robledo, Nicte Selene; Nava-Zavala, Arnulfo Hernán; González-López, Laura; Saldaña-Cruz, Ana Miriam

doi:10.1089/gtmb.2020.0199

Cited by 4 publications

(3 citation statements)

References 36 publications

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“…Of note, SFRP1 is an antagonist of the Wnt/β-catenin signaling pathway; it can inhibit the downward transduction of Wnt and transduction protein (Holdsworth et al 2018 ). The Wnt/β-catenin pathway promotes bone mineralization stimulating proliferation, differentiation, and survival of osteoblasts; it also inhibits osteoclast differentiation and osteocyte activity (Duan and Bonewald 2016 ; Vazquez-Villegas et al 2021 ). Studies have reported that Wnt/β-catenin signaling pathway serves a vital role in OP and that the inhibition of this pathway can improve bone density in an ovariectomized (OVX) rat model (Liu et al 2019 ; Tan et al 2015 ).…”

Section: Discussionmentioning

confidence: 99%

lncRNA SNHG1 induced by SP1 regulates bone remodeling and angiogenesis via sponging miR-181c-5p and modulating SFRP1/Wnt signaling pathway

Rong

Song

et al. 2021

Mol Med

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Background We aimed to investigate the functions and underlying mechanism of lncRNA SNHG1 in bone differentiation and angiogenesis in the development of osteoporosis. Methods The differential gene or proteins expressions were measured by qPCR or western blot assays, respectively. The targeted relationships among molecular were confirmed through luciferase reporter, RIP and ChIP assays, respectively. Alkaline phosphatase (ALP), alizarin red S (ARS) and TRAP staining were performed to measure the osteoblast/osteoclast differentiation of BMSCs. The viability, migration and angiogenesis in BM-EPCs were validated by CCK-8, clone formation, transwell and tube formation assays, respectively. Western blot and immunofluorescence detected the cytosolic/nuclear localization of β-catenin. Ovariectomized (OVX) mice were established to confirm the findings in vitro. Results SNHG1 was enhanced and miR-181c-5p was decreased in serum and femoral tissue from OVX mice. SNHG1 directly inhibited miR-181c-5p to activate Wnt3a/β-catenin signaling by upregulating SFRP1. In addition, knockdown of SNHG1 promoted the osteogenic differentiation of BMSCs by increasing miR-181c-5p. In contrast, SNHG1 overexpression advanced the osteoclast differentiation of BMSCs and inhibited the angiogenesis of BM-EPCs, whereas these effects were all reversed by miR-181c-5p overexpression. In vivo experiments indicated that SNHG1 silencing alleviated osteoporosis through stimulating osteoblastogenesis and inhibiting osteoclastogenesis by modulating miR-181c-5p. Importantly, SNHG1 could be induced by SP1 in BMSCs. Conclusions Collectively, SP1-induced SNHG1 modulated SFRP1/Wnt/β-catenin signaling pathway via sponging miR-181c-5p, thereby inhibiting osteoblast differentiation and angiogenesis while promoting osteoclast formation. Further, SNHG1 silence might provide a potential treatment for osteoporosis. Graphic abstract

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Section: Discussionmentioning

confidence: 99%

lncRNA SNHG1 induced by SP1 regulates bone remodeling and angiogenesis via sponging miR-181c-5p and modulating SFRP1/Wnt signaling pathway

Rong

Song

et al. 2021

Mol Med

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“…The high incidence and fracture rate of OP seriously affect the quality of life of patients. 7 Studies have shown that estrogen can limit bone turnover, inhibit bone resorption, and increase bone density. For postmenopausal women, the incidence of OP increases significantly due to decreased estrogen production as a result of decreased ovarian function.…”

Section: Discussionmentioning

confidence: 99%

“…There have been studies on the relationship between rs851056 locus polymorphism and OP in Mexican-Mestizo postmenopausal women. 7 However, there were few reports on the relationship between SOST protein expression, rs851056, rs1230399 sites polymorphisms and bone mineral density in postmenopausal women with T2DM. The purpose of this study is to explore the relationship between SOST levels, rs851056, rs1230399 polymorphisms and bone mineral density in postmenopausal women with T2DM in Xinjiang.…”

Section: Introductionmentioning

confidence: 99%

Relationship Between Sclerostin (SOST) Expression and Genetic Loci rs851056, rs1230399 Polymorphisms and Bone Mineral Density in Postmenopausal Women with Type 2 Diabetes in Xinjiang

Li¹,

Ren²,

Siyuan³

et al. 2021

DMSO

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Polymorphism of LRP4 Gene (rs9667108) among Post Menopause Women with Osteoporosis

Kavoosian

Asgharian

Asouri

et al. 2023

ijph

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Background: Many studies have been done to identify the factors that influence the development and progression of osteoporosis. One genetic factor is polymorphisms of LRP4 gene. Regarding the lack of comprehensive study on polymorphisms of LRP4 gene in the north of Iran, mainly Mazandaran Province, we decided to investigate the polymorphism of this gene in postmenopausal women with osteoporosis. Methods: This case-control study has been conducted at GhaemShahr Valiasr Hospital on 100 female patients with osteoporosis (average age of 58.1) and 90 healthy females without osteoporosis (average age of 55.2). After sampling and extraction of genomic DNA via of the salt deposition method, the genotype and SNP (rs9667108) polymorphism of LRP4 gene were evaluated with the PCR-RFLP method. Restriction enzymes cut the PCR products. In order to identify patients, their bone mineral density was tested by the DEXA method .The results of digestion (digestion enzyme) were analyzed by MedCalc, SPSS software, Hardy-Weinberg equilibrium, and Chi2. Results: The statistical analysis has shown the significant relationship between SNP (rs9667108) polymorphism and the risk of osteoporosis disease in patients and control groups (P<0.05). In SNP (rs9667108), the GC genotype, compared to GG, increased the risk of disease significantly (1.556 time). Similarly, CC genotype, compared to GG genotype, increased the risk of this disease by 2.091 time. Conclusion: The existence of mutation in the LRP4 gene could increase susceptibility to osteoporosis disease. Moreover, determining this patient's genotype in SNP (rs9667108) can be used to identify individuals who are in endanger osteoporosis.

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Genotypic Analyses of the Sclerostin rs851056 and Dickkopf rs1569198 Polymorphisms in Mexican-Mestizo Postmenopausal Osteoporosis: A Case–Control Study

Cited by 4 publications

References 36 publications

lncRNA SNHG1 induced by SP1 regulates bone remodeling and angiogenesis via sponging miR-181c-5p and modulating SFRP1/Wnt signaling pathway

lncRNA SNHG1 induced by SP1 regulates bone remodeling and angiogenesis via sponging miR-181c-5p and modulating SFRP1/Wnt signaling pathway

Relationship Between Sclerostin (SOST) Expression and Genetic Loci rs851056, rs1230399 Polymorphisms and Bone Mineral Density in Postmenopausal Women with Type 2 Diabetes in Xinjiang

Polymorphism of LRP4 Gene (rs9667108) among Post Menopause Women with Osteoporosis

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