Genotypes of ITS region of rRNA in <i>Microcystis</i> (Cyanobacteria) populations in Erhai Lake (China) and their correlation with eutrophication level

Song, Gaofei; Jiang, Yongguang; Yu, Ge; Li, Renhui

doi:10.1002/jobm.201500080

Cited by 13 publications

(9 citation statements)

References 35 publications

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“…The Microcystis ITS region for Genotype I was identical to that for Microcystis spp. reported from Erhai Lake in China (GenBank accession numbers: KF207342.1 and KF207087.1), Joumine Reservoir in Tunisia (HQ389359.1 and HQ389360.1), and Loire River in France (FJ474921.1) [ 60 – 62 ]. Microcystis Genotype VI had an identical ITS sequence to Microcystis spp.…”

Section: Resultsmentioning

confidence: 99%

Biodiversity of cyanobacteria and other aquatic microorganisms across a freshwater to brackish water gradient determined by shotgun metagenomic sequencing analysis in the San Francisco Estuary, USA

et al. 2018

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Blooms of Microcystis and other harmful cyanobacteria can degrade water quality by producing cyanotoxins or other toxic compounds. The goals of this study were (1) to facilitate understanding of community structure for various aquatic microorganisms in brackish water and freshwater regions with emphasis on cyanobacteria, and (2) to test a hypothesis that Microcystis genotypes that tolerate higher salinity were blooming in brackish water environments during the severe drought, 2014. Shotgun metagenomic analysis revealed that cyanobacteria dominated the brackish water region while bacteria dominated the freshwater region. A group of cyanobacteria (e.g., Aphanizomenon, Microcystis, Planktothrix, Pseudanabaena), bacteria (e.g., Bacillus, Porphyrobacter), and diatoms (Phaeodactylum and Thalassiosira) were abundant in the brackish water region. In contrast, Hassallia (cyanobacteria) and green algae (Nannochloropsis, Chlamydomonas, and Volvox) were abundant in the landward freshwater region. Station variation was also apparent. One landward sampling station located downstream of an urbanized area differed substantially from the other stations in terms of both water chemistry and community structure, with a higher percentage of arthropods, green algae, and eukaryotes. Screening of the Microcystis internal transcribed spacer region revealed six representative genotypes, and two of which were successfully quantified using qPCR (Genotypes I and VI). Both genotypes occurred predominantly in the freshwater region, so the data from this study did not support the hypothesis that salinity tolerant Microcystis genotypes bloomed in the brackish water region in 2014.

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Section: Resultsmentioning

confidence: 99%

Biodiversity of cyanobacteria and other aquatic microorganisms across a freshwater to brackish water gradient determined by shotgun metagenomic sequencing analysis in the San Francisco Estuary, USA

et al. 2018

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“…Compared to Lushui Reservoir, a larger cyanobacterial diversity was observed in Erhai Lake. In a previous investigation, an extremely large number of genotypes were detected for Microcystis in Erhai Lake [ 9 ]. A negative correlation was determined between the Microcystis diversity and eutrophication levels [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, each of above genera contains both toxin-producing and nontoxin-producing species. Microcystis is the most frequently reported bloom-forming cyanobacterium, which often occurs as dense blooms in summer and autumn [ 9 , 10 , 11 ]. In subtropical and temperate lakes, Dolichospermum and Aphanizomenon usually become dominant when the abundance of Microcystis decreases in winter and spring [ 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

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Revealing Cryptic Changes of Cyanobacterial Community Structure in Two Eutrophic Lakes Using eDNA Sequencing

Jiang

Xiao

et al. 2020

IJERPH

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Harmful cyanobacterial blooms pose a risk to human health worldwide. To enhance understanding on the bloom-forming mechanism, the spatiotemporal changes in cyanobacterial diversity and composition in two eutrophic lakes (Erhai Lake and Lushui Reservoir) of China were investigated from 2010 to 2011 by high-throughput sequencing of environmental DNA. For each sample, 118 to 260 cpcBA-IGS operational taxonomic units (OTUs) were obtained. Fifty-two abundant OTUs were identified, which made up 95.2% of the total sequences and were clustered into nine cyanobacterial groups. Although the cyanobacterial communities of both lakes were mainly dominated by Microcystis, Erhai Lake had a higher cyanobacterial diversity. The abundance of mixed Nostocales species was lower than that of Microcystis, whereas Phormidium and Synechococcus were opportunistically dominant. The correlation between the occurrence frequency and relative abundance of OTUs was poorly fitted by the Sloan neutral model. Deterministic processes such as phosphorus availability were shown to have significant effects on the cyanobacterial community structure in Erhai Lake. In summary, the Microcystis-dominated cyanobacterial community was mainly affected by the deterministic process. Opportunistically dominant species have the potential to replace Microcystis and form blooms in eutrophic lakes, indicating the necessity to monitor these species for drinking water safety.

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“…Moreover, it has been shown that the Microcystis abundance has a negative correlation with bacterial (e.g., Actinobacteria) diversity. In Erhai Lake, Song et al identified 473 Microcystis genotypes by seasonal monitoring using internal transcribed spacer (ITS) genes and amplification primers [57]. The data indicated that the early stage of eutrophication in Erhai Lake might result in high diversity of Microcystis due to the negative relationship between the diversity of ITS genotypes and TN/TP.…”

Section: Edna Methods For Eutrophication and Hab Controlmentioning

confidence: 99%

A Review and Perspective of eDNA Application to Eutrophication and HAB Control in Freshwater and Marine Ecosystems

Liu

Zhang

et al. 2020

Microorganisms

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Changing ecological communities in response to anthropogenic activities and climate change has become a worldwide problem. The eutrophication of waterbodies in freshwater and seawater caused by the effects of human activities and nutrient inputs could result in harmful algae blooms (HABs), decreases water quality, reductions in biodiversity and threats to human health. Rapid and accurate monitoring and assessment of aquatic ecosystems are imperative. Environmental DNA (eDNA) analysis using high-throughput sequencing has been demonstrated to be an effective and sensitive assay for detecting and monitoring single or multiple species in different samples. In this study, we review the potential applications of eDNA approaches in controlling and mitigating eutrophication and HABs in freshwater and marine ecosystems. We use recent studies to highlight how eDNA methods have been shown to be a useful tool for providing comprehensive data in studies of eutrophic freshwater and marine environments. We also provide perspectives on using eDNA techniques to reveal molecular mechanisms in biological processes and mitigate eutrophication and HABs in aquatic ecosystems. Finally, we discuss the feasible applications of eDNA for monitoring biodiversity, surveying species communities and providing instructions for the conservation and management of the environment by integration with traditional methods and other advanced techniques.

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Genotypes of ITS region of rRNA in Microcystis (Cyanobacteria) populations in Erhai Lake (China) and their correlation with eutrophication level

Cited by 13 publications

References 35 publications

Biodiversity of cyanobacteria and other aquatic microorganisms across a freshwater to brackish water gradient determined by shotgun metagenomic sequencing analysis in the San Francisco Estuary, USA

Biodiversity of cyanobacteria and other aquatic microorganisms across a freshwater to brackish water gradient determined by shotgun metagenomic sequencing analysis in the San Francisco Estuary, USA

Revealing Cryptic Changes of Cyanobacterial Community Structure in Two Eutrophic Lakes Using eDNA Sequencing

A Review and Perspective of eDNA Application to Eutrophication and HAB Control in Freshwater and Marine Ecosystems

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