Genotypes of human metapneumovirus circulating during 2010‐2012 in children from Panama

Ábrego, Leyda; Mirazo, Santiago; Delfraro, Adriana; Franco, Danilo; Castillo, Marlene; Gaitán, Melissa; Castillo, Juan Antonio; Moreno, Brechla; Pascale, Juan Miguel; Arbiza, Juan

doi:10.1002/jmv.24969

Cited by 7 publications

(6 citation statements)

References 27 publications

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“…In our study, the majority (71.8%; 221/308) of HMPV-positive patients were children between 0 to 5 years old, with more than the half of them under 1 year age (55.7%, 123/221). Consistent with our findings, several studies have reported this sensitivity of children under 5, especially in the first year life, to HMPV infection [22–24]. This highest prevalence in children is believed to be due to a naive immune system for most of HMPV genotypes in early childhood.…”

Section: Discussionsupporting

confidence: 92%

“…A similar genotypic composition has been observed in several other studies [13, 32, 33]. And as in other studies conducted in Saudi Arabia [26], in Croatia [34], in Panama [24] or in Kenya [32], we have no strain belonging to genotype A1, supporting the idea that old lineages have been replaced by emerging genetic strains. Indeed, the A2c subgroup was present in Senegal throughout the study period, except in 2014 where it was not detected probably due to a non-exhaustive sequencing.…”

Section: Discussionsupporting

confidence: 90%

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Epidemiological, clinical and genotypic features of human Metapneumovirus in patients with influenza-like illness in Senegal, 2012 to 2016

et al. 2019

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Background Human metapneumovirus (HMPV) is a causal agent of acute respiratory infection, especially in primarily children. At the clinical level, HMPV is associated to several diseases including bronchitis, croup, pneumonia, bronchiolitis, reactive airway disease, chronic obstructive pulmonary disease and asthma exacerbations, specifically in children less than 5 years. Here, we carried out a retrospective pilot study, based on the processing of nasopharyngeal swabs, with a focus on the epidemiology and molecular characteristics of HMPV in Senegal. Methods This retrospective study was conducted from January 2012 to December 2016. Briefly, all outpatients presenting to healthcare sentinel sites were screened for surveillance enrollment and included if they met criteria for ILI. Naso-oropharyngeal swabs were collected from eligible participants. For viral respiratory pathogens detection, including HMPV, the Anyplex™ II RV16 Detection kit was used. A fragment of the hMPV F gene was targeted for sequencing. Results In total, 8209 patients with ILI were enrolled. Half of them (49.7%) were children under 5 years. Fever was the most common symptom followed by cough, and rhinitis. Three hundred eight patients were positive for HMPV (3.75%). 89 (28.9%) were detected as single infection. In co-infection cases, the most common co-infecting viruses were influenza, adenovirus and rhinovirus. HMPV detection rates in the different age groups varied significantly with the children under 5 years group accounting for 71.7% of positive patients. The temporal distribution pattern for HMPV infection showed a clear seasonal pattern with a higher activity during the rainy period (July–September). Phylogenetic analyses revealed that HMPV specimens circulating in Senegal were distributed into the two main genetic lineages, A and B. We also noted a co-circulation of both genetic lineages during the whole study period except in 2014. Conclusion In summary, the present study characterized the recent prevalence, seasonality and genetic diversity of HMPV in a large outpatient population presented with ILI in Senegal between 2012 and 2016. Globally our results show a clear seasonal circulation pattern of HMPV in Senegal. Our findings identified children less than 5 years as more susceptible group to HMPV infection. Molecular studies identified A2, B1 and B2 as the major genotypes circulating.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 90%

Epidemiological, clinical and genotypic features of human Metapneumovirus in patients with influenza-like illness in Senegal, 2012 to 2016

et al. 2019

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“…We identified the hMPV A 2 subtype and our results showed that it circulated in Córdoba during 2011. We did not detect subtypes A1, B1 and B2; a reason for this may have been we were not able to get high-quality RNA and/or optimal sequences from the years 2012 and 2013, however, other authors demonstrated that these subtypes are seldom reported [40]. In our country, Galiano (2006) demonstrated the circulation of A 1 , A 2 and B 2 subtypes; Velez Rueda (2013) showed that A 2 and B 2 subtypes co-circulated during 2009-2010, while hMPV A 2 was detected in 2011 [16,28].…”

Section: Discussionmentioning

confidence: 69%

Human Metapneumovirus: Epidemiology and genotype diversity in children and adult patients with respiratory infection in Córdoba, Argentina

et al. 2020

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Human Metapneumovirus (hMPV) is responsible for acute respiratory infections in humans, with clinical and epidemiological relevance in pediatric, elderly, and immunocompromised populations. These features are largely unknown in Córdoba, Argentina and in adults in general. Hence, our goal was to broadly characterize hMPV infection in patients of all ages hospitalized with acute respiratory infections in Córdoba, Argentina, including epidemiology, clinical features and genetic diversity. Nasopharyngeal secretions were obtained from 795 patients during 2011–2013, 621 patients were 0–25 years old and 174 were 26–85 years old. HMPV was assayed by RT-PCR and other respiratory viruses by indirect immunofluorescence. Local strains were identified by sequence analysis. Human Metapneumovirus was detected in 20.3% (161/795) patients, 13.1% as single infections and 7.2% in co-infections, more frequently with Respiratory Syncytial Virus. HMPV circulated during late winter and spring in all age patients, but mainly in children under 4 years old in 71.4% (115/161) and adults between 26 and 59 years old in 12.4% (20/161). The most prevalent diagnosis was mild acute respiratory infection in 59.6% (96/161) and bronchiolitis in 9.3% (15/161). Local strains were clustered within A2 subtype; they presented 73–100% identities among them, showing a high degree of homology compared to isolations from neighboring countries. We demonstrate that hMPV circulated among all age patients with respiratory infection during 2011–2013 in Córdoba, contributing to the understanding of this virus, its diagnosis and patient handling in local health-care centers.

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“…Panama included HMPV surveillance in 2010 as part of the National Epidemiologic Surveillance System. A previous study [27] described a HMPV frequency among ≤5-year-olds hospitalized patients with ARI of 1.3% in 2011 and 13.9% in 2012, which may suggest important fluctuations over time. We did not detect HMPV in hospitalized children with ARI in 2016.…”

Section: Discussionmentioning

confidence: 87%

Epidemiology of viral respiratory infections in a pediatric reference hospital in Central Panama

Núñez-Samudio

Landires

2021

BMC Infect Dis

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Background Acute respiratory infections (ARIs) are a worldwide public health problem. It is estimated that up to 80% of cases of ARIs are caused by viruses. In Central America, however, we identified few epidemiologic studies on the main ARI-related viruses in hospitalized children. Methods This study retrospectively analyzed the clinical charts of patients ages 29 days to 14 years admitted with diagnoses of ARIs in a pediatric reference hospital in central Panama during 2016. The variables analyzed were age, sex, signs, symptoms, and diagnosis at admission. Samples of patients to whom a viral panel was indicated were analyzed via quantitative polymerase chain reaction, qPCR. Results The most common virus was respiratory syncytial virus (RSV; 25.9%), followed by influenza A virus (10.6%), rhinovirus (10.6%), parainfluenza type 3 (PIV-3; 8.2%) and adenovirus (5.9%). However, virus detection varied with patient age and season. RSV and Influenza virus were respectively identified mainly during July–November and May–July. All cases of viral co-infection occurred in children < 5-years-old. Both influenza A (H1N1) pdm09 and rhinovirus were detected in all pediatric ages analyzed in this study, unlike RSV and PIV-3, which were only present in children < 5-years-old. Conclusions This study analyzed the epidemiological patterns of different respiratory viruses in pediatric patients with ARI from central Panama and found that the prevalence of the specific respiratory viruses identified varied with season and age. The most common viruses were RSV, influenza A, and rhinovirus. There were no reports of human metapneumovirus associated with ARI, which may be explained by the time and geographic location of the study. Knowledge of the local epidemiology of respiratory viruses in tropical countries is helpful in forecasting the peaks of hospitalizations due to ARIs and may help improve prevention efforts aiming at respiratory disease control in these settings.

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Genotypes of human metapneumovirus circulating during 2010‐2012 in children from Panama

Cited by 7 publications

References 27 publications

Epidemiological, clinical and genotypic features of human Metapneumovirus in patients with influenza-like illness in Senegal, 2012 to 2016

Epidemiological, clinical and genotypic features of human Metapneumovirus in patients with influenza-like illness in Senegal, 2012 to 2016

Human Metapneumovirus: Epidemiology and genotype diversity in children and adult patients with respiratory infection in Córdoba, Argentina

Epidemiology of viral respiratory infections in a pediatric reference hospital in Central Panama

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