Genotype versus Phenotype Variability in Chlorella and Micractinium (Chlorophyta, Trebouxiophyceae)

Luo, Wei; Pflugmacher, Stephan; Pröschold, Thomas; Walz, Norbert; Krienitz, Lothar

doi:10.1016/j.protis.2006.05.006

Cited by 128 publications

(124 citation statements)

References 45 publications

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“…The ITS is often used to base taxonomic measures on the occurrence of complementary base changes within the secondary structure of either ITS1 or ITS2 or both (luo et al 2006;Bock et al 2011a,b;da silva et al 2016). darienko et al (2015 even recommended the use of the V9 and ITS region as barcode for the green algae Coccomyxa.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity in chrysophytes: Comparison of different gene markers

Böck¹,

Chatzinotas²,

Boenigk³

2017

Fottea

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Abstract:Chrysophytes are a diverse group of protists belonging to the Stramenopiles. They encompass a broad range of nutrition strategies (phototrophy, mixotrophy and heterotrophy) and morphological forms (unicellular, colonial, branched, non-scaled to silica-scaled). However, taxonomy, identification and delineation of genera and species are hampered by the polyphyletic origin of several morphotypes and the low resolution of morphological features; moreover, description of these features requires in many cases special equipment like TEM or SEM. To test the congruent phylogeny, genetic resolution and to reveal the genetic diversity of chrysophytes, we evaluated in this study different gene markers, in particular SSU and LSU rRNA genes, ITS, and COI in terms of phylogenetic clustering, separation of phylogenetic lineages and genetic distances within and between clades. Our results showed that the general topology of the SSU rRNA gene phylogeny is similar to that of the LSU rRNA gene phylogeny, with a higher genetic divergence for the latter. The topology of the COI phylogeny differed from the ribosomal data, and showed the highest genetic divergence within groups and between groups for all compared markers. The 5.8S rRNA gene offered only a limited phylogenetic resolution with low genetic divergence. ITS showed a high genetic divergence, but phylogenetic analyses were hampered by high variance in sequence lengths between clades.

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Section: Discussionmentioning

confidence: 99%

Genetic diversity in chrysophytes: Comparison of different gene markers

Böck¹,

Chatzinotas²,

Boenigk³

2017

Fottea

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“…NIES-455, and the ancestral algal strain of the CET microcosm. Sequences and structures were compared visually to identify the generic-level synapomorphic signatures of Chlorella and Micractinium proposed by Luo et al (2006). Strain sequences were identified and renamed according to the results, then deposited in the NCBI database (GenBank) under the following accession numbers: Micractinium sp.…”

Section: Molecular Analyses and Genbank Accession Numbersmentioning

confidence: 99%

“…For example, free-living Micractinium (of the Chlorella clade) forms bristles on its cell wall (Krienitz et al, 2004), which is considered to be an adaptation that inhibits predation by e.g. rotifers (Luo et al, 2006). The bristles may also prohibit cells from being ingested by prospective host organisms, reducing the opportunity for the potential establishment of endosymbiosis.…”

Section: Introductionmentioning

confidence: 99%

“…As molecular markers we used the rDNA gene (SSU 18S, ITS1, 5.8S and ITS2) and the secondary structure of the rRNA ITS2 region, as proposed in recent studies (Luo et al, 2006(Luo et al, , 2009Hoshina et al, 2010;Pröschold et al, 2011). For comparison, we also sequenced some strains from the NIES culture collection (Micractinium pusillum NIES-151, Micractinium sp.…”

Section: Introductionmentioning

confidence: 99%

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The phylogenetic position and phenotypic changes of a Chlorella-like alga during 5-year microcosm culture

Germond

Hata

Fujikawa

et al. 2013

European Journal of Phycology

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Recent phylogenetic studies of Chlorella-like algae revealed that this group has diversified into free-living and endosymbiotic niches, the latter within protists and invertebrate organisms. Our previous studies using a long-term culture composed of an alga (Chlorella vulgaris, rDNA sequence unknown), a bacterium (Escherichia coli) and a ciliate (Tetrahymena thermophila), suggested that some clones in the algal population developed an endosymbiotic ability with T. thermophila while others developed an ectosymbiotic ability with E. coli from a non-associated stage. In this paper, an rDNA (18S, ITS1, 5.8S, ITS2)-based phylogeny of the ancestral strain and derived clones isolated from 5-year microcosm cultures was constructed and revealed that the alga belongs to the genus Micractinium. This result was supported by analysis of the secondary structure of the rRNA gene. No difference was observed in the sequence between the ancestral and derived clones. On the other hand, some morphological and physiological traits of the ancestral strain and the derived clones were analysed and revealed that several phenotypic changes had occurred among the algal clones. In particular, the derived clones obtained from long-term cultures had increased in cell size, changed in their ability to grow at pH 4.0, and developed a tendency for cell-aggregation or sedimentation.

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“…This capacity of ubiquitous dispersal is driven by huge population sizes leading to low probability of local extinction and, then the most microorganisms with size smaller than 1 mm have worldwide distribution while those larger than 10 mm are much less abundant and rarely cosmopolitan (Fenchel & Finlay 2003, Finlay & Fenchel 2004. Although some molecular studies support the "cosmopolitan hypothesis" (Daugjberg et al 2000) for microalgae this idea is controversy because more recently available data suggest that micro-organisms (including protists and algae) have distribution patterns similar to those known from higher plants and animals, and that these patterns reflect historical (Gondwanan/Laurasian), ecological (tropical/temperate), and continental/local conditions (Coleman 2002, Foissner 2006, Luo et al 2006.…”

Section: Discussionmentioning

confidence: 99%

Flagellate green algae from four water bodies in the state of Rio de Janeiro, Southeast Brazil

Menezes

Mattos

2008

Hoehnea

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-(Flagellate green algae from four water bodies in the state of Rio de Janeiro, Southeast Brazil). Floristic survey of flagellate green algae (Chlorophyceae and Prasinophyceae) from four water bodies in the state of Rio de Janeiro, southeast Brazil. Samples were collected bimonthly from September 1988 to August 1991. Thirty-five taxa of which 31 in Chlorophyceae (24 Chlamydomonadales and seven Volvocales) and four in Prasinophyceae (Polyblepharidales) were identified. Twenty-three taxa are new records for Brazil and 31 are registered for the first time for Rio de Janeiro State. On basis of cell shape, location of the nucleus and degree of longitudinal striations on the chloroplast surface a new combination, Vitreochlamys lefevrei (Bourr.) Menezes & C. Bicudo, is proposed. Integrating studies among morphological analysis on natural/cultured material and characterizing of reproduction/life cycle as well the correlating between the occurrence of the species and environmental conditions are needed in order to better knowledge the degree of phenotypic plasticity within the members of the flagellate green algae in the country.

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Genotype versus Phenotype Variability in Chlorella and Micractinium (Chlorophyta, Trebouxiophyceae)

Cited by 128 publications

References 45 publications

Genetic diversity in chrysophytes: Comparison of different gene markers

Genetic diversity in chrysophytes: Comparison of different gene markers

The phylogenetic position and phenotypic changes of a Chlorella-like alga during 5-year microcosm culture

Flagellate green algae from four water bodies in the state of Rio de Janeiro, Southeast Brazil

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