Genotype‐Specific Persistence of Genital Human Papillomavirus (HPV) Infections in Women Followed for 6 Years in the Finnish Family HPV Study

Louvanto, Karolina; Rintala, Marjut; Syrjänen, Kari; Grénman, Seija; Syrjänen, Stina

doi:10.1086/653826

Cited by 74 publications

(91 citation statements)

References 39 publications

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“…The Finnish Family HPV Study is a prospective cohort study conducted jointly by the Department of Obstetrics and Gynecology, Turku University Hospital (TUH), and the Institute of Dentistry, Faculty of Medicine, University of Turku. The subjects in this cohort are pregnant women who were recruited at a minimum of 36 weeks of their index (baseline) pregnancy (16) and followed up for 6 years (mean, 54.9 months; standard deviation [SD], 27.3 months; median, 62.4 months; range, 1.6 to 94.5 months) after delivery (10). The Joint Commission on Ethics of Turku University and TUH has approved the study protocol and its amendments (no.…”

Section: Methodsmentioning

confidence: 99%

Genotype-Specific Clearance of Genital Human Papillomavirus (HPV) Infections among Mothers in the Finnish Family HPV Study

et al. 2010

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The majority of cervical human papillomavirus (HPV) infections in young women are transient, but whether the clearance differs among different HPV genotypes and the different factors predicting genotype-specific clearance are partly unknown. In the Finnish Family HPV Study, 131 of 252 women (mean age, 25.5 years) cleared their infection during the prospective follow-up of 6 years (median, 62.4 months; range, 1.6 to 94.5 months). Cervical scrapings collected at each visit were tested for 24 low-risk and high-risk (HR) HPV types with multiplex HPV genotyping. Poison regression (panel data) was used to estimate predictors for the clearance of species 7 and 9 HPV genotypes. Of all HPV genotypes detected in these women, multiple-type and HPV type 16 (HPV16) infections showed clearance least frequently (46.1% and 50.5%, respectively). The actuarial and crude mean times to first clearance were variable among different genotypes. The actuarial clearance rate (events/person-time at risk) was highest for HPV16 and multiple-type infections, while HPV66 and -82 had the highest crude clearance rate. Independent predictors increasing type-specific clearance of species 7/9 HPV genotypes were older age (incidence rate ratio [IRR] ‫؍‬ 1.1; 95% confidence interval [95% CI], 1.03 to 1.18; P ‫؍‬ 0.002) and baseline oral HR HPV DNA-negative status (IRR ‫؍‬ 2.94; 95% CI, 1.03 to 8.36; P ‫؍‬ 0.042), while a higher number of sexual partners during the follow-up decreased the probability of clearance (IIR ‫؍‬ 0.35; 95% CI, 0.15 to 0.83; P ‫؍‬ 0.018). To conclude, HPV16 and multiple-type infections showed the lowest clearance among young mothers. Increasing age and negative oral HR HPV DNA status at baseline were associated with increased clearance, whereas a higher number of current sexual partners decreased the probability of species 7/9 HPV genotype clearance.Genital human papillomavirus (HPV) infections are transient in most cases (3, 7). Most studies on HPV clearance have addressed high-risk (HR) HPV types collectively and/or have compared clearance between HR and low-risk (LR) HPV types (3,4,12,13,17,18,21,23,24). Earlier data suggest that HR HPV infections usually clear more slowly than LR HPV infections (4,25) and that the likelihood of an infection not clearing increases in parallel with its duration (7, 13).It was not until recently that data on HPV clearance at the genotype level were available (5,9,19,25). The results indicate that infection with HPV type 16 (HPV16) has the lowest tendency for clearance. Accurate data on actual and crude clearance times and clearance rates (CRs) for individual genotypes are needed to understand the natural history of HPV infections.The present study is one of the first to assess the frequency of HPV type-specific clearance as well as the actuarial and crude clearance times and clearance rates for the 24 most common LR and HR HPV genotypes. The study was performed among newly delivered mothers who were followed up for 6 years in the Finnish Family HPV Study. In addition, predictors of species ...

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Section: Methodsmentioning

confidence: 99%

Genotype-Specific Clearance of Genital Human Papillomavirus (HPV) Infections among Mothers in the Finnish Family HPV Study

et al. 2010

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“…The Finnish Family HPV Study is a longitudinal cohort study conducted at the University of Turku and Turku University Hospital, Finland, as previously detailed [16,17,18] Oral scrapings were taken from the buccal mucosa of both cheeks and from the upper and lower vestibular area using a small brush (Cytobrush®, MedScan, Malmö, Sweden). The brush was immersed in 80% ethanol, frozen and stored at -70°C until analysed [16].…”

Section: Study Population and Clinical Samplesmentioning

confidence: 99%

“…The brush was immersed in 80% ethanol, frozen and stored at -70°C until analysed [16]. The oral samples were collected at seven time points: the baseline before delivery, and at 2-, 6-, 12-, 24-, 36-months and 6 years after delivery [16,17,18].…”

Section: Study Population and Clinical Samplesmentioning

confidence: 99%

“…Oral epithelial cells can be readily transformed by HPV, with potential to develop oral squamous cell carcinoma [13,14,15]. The Finnish HPV Family Study has assessed the dynamics of HPV transmission within regular families in a longitudinal setting, as previously described [16,17,18]. In the present study, we analysed HSV-1 and HSV-2 DNA in oral mucosal scrapings of the mothers included in the Finnish Family HPV Study, with special emphasis on co-carriage of these two viruses in oral mucosa.…”

Section: Introductionmentioning

confidence: 99%

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Carriage of herpes simplex virus and human papillomavirus in oral mucosa is rare in young women: A long-term prospective follow-up

Mäki

Paavilainen

Grénman

et al. 2015

Journal of Clinical Virology

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“…In the past decade, researchers have combined Luminex technology with GP5ϩ/6ϩ (2,3,4,5), MY09/11 (6), PGMY09/11 (7), and other primer systems. These various primer systems all have extensive histories of use for HPV typing in the research setting predating their employment in Luminex-based approaches.…”

Section: H Uman Papillomavirus (Hpv) Is the Most Common Sexually Tranmentioning

confidence: 99%

Development of a Novel Liquid Bead Array Human Papillomavirus Genotyping Assay (PGMY-LX) and Comparison with Linear Array for Continuity in Longitudinal Cohort Studies

Farhat

Scott

et al. 2015

J Clin Microbiol

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Studies of the natural history of human papillomavirus (HPV) infection require reproducible, type-specific testing of the viral types that infect cervical tissue; Linear Array (LA) is one method that has been widely used. We sought to develop a cost-effective, high-throughput alternative using the same PGMY09/11 primer/probe system and offering sensitivity and specificity comparable to those with LA to ensure continuity in longitudinal studies. We report here on a Luminex-based approach, PGMY-LX, that offers type-specific detection of 33 oncogenic and nononcogenic types. Detection of HPV type-specific plasmid DNA was highly specific, with high signal-to-noise ratios for all types except nononcogenic type 40 and no cross-reactivity between types. H uman papillomavirus (HPV) is the most common sexually transmitted infection among sexually active individuals.More than 40 genotypes infect the mucosal epithelium and have been divided into two main groups, "high-risk" (HR) and "lowrisk" (LR), according to their oncogenic potential (1). Persistent infection with one or more HR types is a prerequisite for the development of cervical cancer or precancerous lesions.Studies seeking to understand the natural history of HPV infection and associated risk factors require type-specific testing of the HR and LR types that infect cervical tissue. Such studies have historically relied on a combination of in-house methods and commercial tests, such as Inno-LiPA and Roche Molecular Diagnostics Linear Array (LA), to detect type-specific HPV DNA sequences. The latter are both line blot assays and distinguish 28 and 37 specific HPV genotypes, respectively. LA, with its ability to individually identify the greatest number of HPV types is well established and widely used. It is a PCR-driven method employing the PGMY09/11 primer system, which uses pooled, nondegenerate primers to amplify part of the highly conserved L1 gene of the viral capsid. Detection is based on solid-phase hybridization of amplified HPV sequences to a slot blot membrane.The introduction of Luminex technology, allowing multiplexed testing of up to 100 unique assays within a single, smallvolume sample, brought researchers the possibility of developing a high-throughput HPV genotyping assay suitable for cohort studies. In the past decade, researchers have combined Luminex technology with GP5ϩ/6ϩ (2, 3, 4, 5), MY09/11 (6), PGMY09/11 (7), and other primer systems. These various primer systems all have extensive histories of use for HPV typing in the research setting predating their employment in Luminex-based approaches. Because the various primer systems have been shown to have different performance characteristics in type-specific HPV detection (8, 9), continuity within a prospective study generally is best facilitated by using the same primer set throughout.Since 2000, we have used the PGMY09/11-based LA approach, as well as its precommercial predecessor, reverse line blot (10), to prospectively characterize the natural history of cervical HPV infection in adolescents...

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Genotype‐Specific Persistence of Genital Human Papillomavirus (HPV) Infections in Women Followed for 6 Years in the Finnish Family HPV Study

Abstract: HPV16 was the most frequent persisting HPV genotype followed by multiple infections. Early initiation of smoking, practicing oral sex and older age increase the risk for persistence of species 7 and 9 HPV genotypes.

Cited by 74 publications

References 39 publications

Genotype-Specific Clearance of Genital Human Papillomavirus (HPV) Infections among Mothers in the Finnish Family HPV Study

Genotype-Specific Clearance of Genital Human Papillomavirus (HPV) Infections among Mothers in the Finnish Family HPV Study

Carriage of herpes simplex virus and human papillomavirus in oral mucosa is rare in young women: A long-term prospective follow-up

Development of a Novel Liquid Bead Array Human Papillomavirus Genotyping Assay (PGMY-LX) and Comparison with Linear Array for Continuity in Longitudinal Cohort Studies

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