Genotype distribution of human papillomavirus (HPV) in histological sections of cervical intraepithelial neoplasia and invasive cervical carcinoma in Madrid, Spain

Abstract

BackgroundHuman Papillomavirus (HPV) genotype distribution and co-infection occurrence was studied in cervical specimens from the city of Madrid (Spain), as a contribution to the knowledge of Human Papillomavirus genotype distribution and prevalence of carcinogenic HPV types in cervical lesions in Spain.MethodsA total of 533 abnormal specimens, from the Hospital General Universitario “Gregorio Marañón” of Madrid, were studied. These included 19 benign lesions, 349 cervical intraepithelial neoplasias 1 (CIN1), 158 CIN2-3 and 7 invasive cervical carcinomas (ICC). HPV genotyping was performed using PCR and tube array hybridization.ResultsWe detected 20 different HPV types: 13 carcinogenic high-risk HPV types (HR-HPVs), 2 probably carcinogenic high-risk HPV types (PHR-HPVs) and 5 carcinogenic low-risk HPV types (LR-HPVs). The most frequent HPV genotypes found in all specimens were HPV16 (26.0%), 31 (10.7%) and 58 (8.0%). HPV 18 was only detected in 5.0%. Co-infections were found in 30.7% of CIN 1 and 18.4% cases of CIN2-3. The highest percentage of HR HPVs was found in those specimens with a CIN2-3 lesion (93.7%).ConclusionAs our study shows the current tetravalent vaccine could be effective in our geographical area for preventing all the invasive cervical carcinomas. In addition, upon the estimates of the important presence of other HR-HPV types – such as 31, 58, 33 and 52 – in different preneoplasic lesions the effectiveness of HPV vaccination in our geographical area, and others with similar genotype distribution, should be limited.

HPV prevalence and type‐distribution in cervical cancer and premalignant lesions of the cervix: A population‐based study from Northern Ireland

“…The overall prevalence of any HPV in SCC samples was 92.2% and was 68.0% across all cervical samples investigated. HR‐HPV was detected in 64.8% of all samples with HPV‐16 being the most common HPV genotype identified, which is consistent with that described elsewhere in CIN cases across Europe in countries such as Spain, France, and Germany [García‐Espinosa et al, ; Monsonego et al, ; de Jonge et al, ; Leinonen et al, ; Rössler et al, ] and in invasive disease internationally [Bosch et al, ; de Sanjose et al, ; Li et al, ]. As previously reported the prevalence of HPV increased through CIN I–III lesions in NI [Anderson et al, ] from 48.1% in CIN I to 81.3% in CIN III cases.…”

Distribution of High-Risk Human Papillomavirus Genotypes in Precancerous Cervical Lesions in Ouagadougou, Burkina Faso

“…We found no significant link between the histological lesions and the HPV carrier. In our study, the prevalence rate of precancerous lesions was significantly lower than that of most studies [13] [15] [21]. This could be explained by the size of our sample but especially by the quality of our samples; or other factors other than HPV such as bacteria, parasites, fungi, other virus, or simply other HPV genotypes that our detection kit could not characterize.…”

“…Garcia-Espinosa et al found 18.4% of multiple infections. [21] In South Africa, 24% of cases of multiple infections were observed [18]. In Burkina Faso, 78.03% of cases of multiple infections were observed, but 90.1% of the patients were HIV-infected [10].…”

Shift in prevalence of HPV types in cervical cytology specimens in the era of HPV vaccination

“…As previously reported, the incidences of particular HR-HPV infections were observed to increase in association with the severity of the cervical intraepithelial neoplasia in the present study (5,35,42,43). In previous studies, each high-grade cervical neoplasia was attributed to a single HR-HPV infection, and HPV16 and 31 were the predominant HR-HPV types (44). These results are similar to the cytological findings of the present study, which demonstrated that the most frequent HPV types were HPV16 and 31.…”