Genotype Distribution and Prevalence of Human Papillomavirus Among Russian Women in Rostov, Southern Federal District of Russia

Abstract

HPV burden is a marker for cervical neoplasms and cancer. Prevalence of HPV infection and HPV genotypes varies amongst different regions. This research was aimed to investigate the age distribution patterns and prevalence of high-risk HPV genotypes among Rostov-on-Don women. Scrapings of epithelial cells obtained from the urogenital tract of 5655 women. Total DNA was extracted by the sorbent method; Real-Time PCR was used to investigate the HPV load and HPV genotyping. HPV was found in 40% of the DNA samples. The HPV infection was most prevalent among women age ≤ 20 years (55,26%) compared to 27,34% of women older than 40 years (p = 0,001). The prevalence of HPV 16/18 types was almost the same in all age groups. More 50% of women with a high HPV load were women at age group 20-30 years old. Among 254 women, 79,13% had single HPV type infection, and 20,86% had multiple HPV infection. The most frequent of high-risk HPV types were 16, 51 and 31 types. The most common variant of genotypes co-exist for multiple HPV infections were genotypes of A7 + A9 phylogenetic groups (30,18 %). Multiple HPV infections were the most prevalent (67,92%) in women at age group 20-30 years old. We concluded the prevalence of HPV infection among younger women was the highest and declined gradually with age among Rostov-on-Don women.