Genotype Differencies in Direct Plant Regeneration from Stem Explants of <i>Populus tremula</i> in Turkey

Gözükırmızı, Nermin; Bajroviç, Kasım; Ipekci, Z.; Boydak, Melih; Akalp, Tahsin; Tunçtaner, Korhan; Balkan, Hülya; Tanriyar, H.; Çalıkoėlu, Mehmet; Oėraş, Tijen; Özden, Öznur; Tulukçu, Mümtaz; Tank, Turan

doi:10.1007/bf02760313

Cited by 7 publications

(8 citation statements)

References 10 publications

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“…Growth and differentiation response is not only controlled by the cultural environment, but is also dependent upon the genotype [11]. In vitro regeneration has been attempted in several poplar species using different explants such as leaves [8,11–14], petioles [15], internodes [8,14], stems [5,16], roots [8,11], and shoot tips [17]. The leaf disc transformation method [18] has been widely used in plant genetic engineering.…”

Section: Introductionmentioning

confidence: 99%

Efficient Agrobacterium-Mediated Transformation of Hybrid Poplar Populus davidiana Dode × Populus bollena Lauche

Han

Shu-Rong

Kong

et al. 2013

IJMS

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Poplar is a model organism for high in vitro regeneration in woody plants. We have chosen a hybrid poplar Populus davidiana Dode × Populus bollena Lauche. By optimizing the Murashige and Skoog medium with (0.3 mg/L) 6-benzylaminopurine and (0.08 mg/L) naphthaleneacetic acid, we have achieved the highest frequency (90%) for shoot regeneration from poplar leaves. It was also important to improve the transformation efficiency of poplar for genetic breeding and other applications. In this study, we found a significant improvement of the transformation frequency by controlling the leaf age. Transformation efficiency was enhanced by optimizing the Agrobacterium concentration (OD600 = 0.8–1.0) and an infection time (20–30 min). According to transmission electron microscopy observations, there were more Agrobacterium invasions in the 30-day-old leaf explants than in 60-day-old and 90-day-old explants. Using the green fluorescent protein (GFP) marker, the expression of MD–GFP fusion proteins in the leaf, shoot, and root of hybrid poplar P. davidiana Dode × P. bollena Lauche was visualized for confirmation of transgene integration. Southern and Northern blot analysis also showed the integration of T-DNA into the genome and gene expression of transgenic plants. Our results suggest that younger leaves had higher transformation efficiency (~30%) than older leaves (10%).

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Section: Introductionmentioning

confidence: 99%

Efficient Agrobacterium-Mediated Transformation of Hybrid Poplar Populus davidiana Dode × Populus bollena Lauche

Han

Shu-Rong

Kong

et al. 2013

IJMS

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“…As emphasized by Gozukirmizi et al (1998) strong genotype dependence for in vitro regeneration was also observed. According to the study, nine Populus tremula clones were tested for direct regeneration, with the Dursunbey genotype giving the best results.…”

Section: Discussionmentioning

confidence: 87%

“…Han et al (1994) found the rooting ratio for P. trichocarpa x P. deltoids to be 67% on WPM with 10 µM IBA. Gozukirmizi et al (1998) found that, WPM with 0.5 mg/l IBA induced rooting after 2 weeks at 100% for P. tremula. According to these results for four clones as shown in Table 5, rooting was statistically affected by clones.…”

Section: Discussionmentioning

confidence: 99%

“…Solid WPM for direct and indirect regeneration was used and solid MS and WPM for rooting. According to Gozukirmizi et al (1998), shoot regeneration of P. tremula on WPM with 1 mg/l zeatine was found to be 50% more efficient than with aspen culture medium (ACM) (with 0.5 mg/l BA and 0.02 mg/l NAA) and MS (with 0.05 mg/l NAA and 2.25 mg/l BA). Similarly, Han et al (1994) used WPM with 5 µM trans-zeatine for direct shoot regeneration in their study on the F2 family of Populus trichocarpa × P. deltoides.…”

Section: Discussionmentioning

confidence: 99%

“…The poplar is an important model for woody perennial biotechnology because it is amenable to in vitro culture and genetic engineering through Agrobacterium-mediated transformation (Jehan et al, 1994;Gozukirmizi et al, 1998;Confalonieri et al, 2000;Han et al, 2000;Dai et al, 2003). There are now numerous techniques available for rapid and extensive multiplication of elite and desirable plant species in vitro.…”

Section: Introductionmentioning

confidence: 99%

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Direct and indirect plant regeneration from various explants of eastern cottonwood clones (Populus deltoides Bartram ex Marsh.) with tissue culture

Çavuşoğlu¹,

Ipekci-Altas²,

Bajroviç³

et al. 2011

Afr. J. Biotechnol.

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Antisense Expression of a Caffeic AcidO-methyltransferase ofStylosanthes humilisin Transgenic Poplar: Effect of Expression onO-methyltransferase Activity and Lignin Composition

Bajroviç

Kazan

Ipekci

et al. 1999

Journal of Forest Research

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Genotype Differencies in Direct Plant Regeneration from Stem Explants of Populus tremula in Turkey

Cited by 7 publications

References 10 publications

Efficient Agrobacterium-Mediated Transformation of Hybrid Poplar Populus davidiana Dode × Populus bollena Lauche

Efficient Agrobacterium-Mediated Transformation of Hybrid Poplar Populus davidiana Dode × Populus bollena Lauche

Direct and indirect plant regeneration from various explants of eastern cottonwood clones (Populus deltoides Bartram ex Marsh.) with tissue culture

Antisense Expression of a Caffeic AcidO-methyltransferase ofStylosanthes humilisin Transgenic Poplar: Effect of Expression onO-methyltransferase Activity and Lignin Composition

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