Genotype‐dependent transcriptional activation of novel repetitive elements during cold acclimation of alfalfa (<i>Medicago sativa</i>)

Ivashuta, Sergey; Naumkina, Marina; Gau, Mitsuru; Uchiyama, Kazuhiro; Isobe, Sachiko; Mizukami, Yuko; Shimamoto, Yuta

doi:10.1046/j.1365-313x.2002.01383.x

Cited by 40 publications

(25 citation statements)

References 47 publications

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“…Rocha et al (2005) found three mRNA transcript fragments, over expressed in wheat roots treated with DON, with full or partial homology to retrotransposons. This and other researches (Ivashuta et al 2002) implies the possibility of genetic or epigenetic transposon activation in plant cells in response to stress. Therefore, Fadl-Allah et al (2007) and Mohammed et al (2008) speculated that the existed variation of DNA which have been detected by RAPD-PCR after treating with DON and AFB 1 might be a DNA structural changes, which indicated their genotoxic effect on wheat.…”

Section: Resultsmentioning

confidence: 85%

Molecular cytogenetical effects of some mycotoxins to chromosomes of Triticum durum using RAPD-PCR fluorescence in situ hybridization

et al. 2009

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ABSTRACT. PCR-amplified DNA using the random primer OPB-06 was used as a probe for Fluorescence in situ hybridization (FISH). Signals of hybridized sites were observed in both interphase and metaphase plates after hybridization. The FISH signals of the metaphase chromosomes were confirmed as two yellow-color signals hybridized with the probe on two adjacent loci at interphase of the control plates. FISH analysis of the metaphase plates of 25µg/ml deoxynivlenol (DON) and aflatoxin B 1 (AFB 1 ) treated plants revealed 85.7% and 89.3%, respectively had no signals. Of the metaphase plates of plants treated with AFB 1 , 3.6% had one terminal signal while 7.1% revealed two signals and no signals were observed in 89.3% plates. Regarding the interphase nuclei, only one signal was observed in 42.1% of the plates where two signals were observed in 57.8% interphase plates. 10.7% metaphase plates of plants treated with DON had one terminal signal while 3.6% exhibit two signals and no signals were observed in 85.7% plates of metaphase. 44.1% of the interphase nuclei had one signal while 53.2% plates reveal two signals and four signals were observed in 2.7% interphase nuclei. Disappearance of FISH signals in some metaphase plates of plants treated with the two investigated toxins indicates the toxigenic effect of these mycotoxins on metaphase chromosomes of wheat.

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Section: Resultsmentioning

confidence: 85%

Molecular cytogenetical effects of some mycotoxins to chromosomes of Triticum durum using RAPD-PCR fluorescence in situ hybridization

et al. 2009

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“…The level of transcript activation was genotype-specific. This and other research (Ivashuta et al 2002) implies that there may be genetic or epigenetic transposon activation in plant cells in response to stress and the degree of activation may be genotype-specific (perhaps due to genotype-specific sites of genomic integration). Even if they are transpositionally inactive, the transcription of redundant RNA could affect the expression of other genes through RNA-RNA interaction or RNA-directed DNA methylation (Grant 1999, Wolffe andMatzke 1999).…”

Section: Activation Of Wheat Retrotransposon-like Transcriptsmentioning

confidence: 86%

Effects of trichothecene mycotoxins on eukaryotic cells: A review

Rocha

Ansari²,

Doohan³

2005

Food Additives and Contaminants

492

298

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The major products of the trichothecene mycotoxin biosynthetic pathway produced in a species-and sometimes isolatespecific manner by cereal-pathogenic Fusarium fungi include T-2 toxin, diacetoxyscirpenol, deoxynivalenol and nivalenol. This paper briefly reviews the major effects of such trichothecenes on the gross morphology, cytology and molecular signalling within eukaryotic cells. The gross toxic effects of select trichothecenes on animals include growth retardation, reduced ovarian function and reproductive disorders, immuno-compromization, feed refusal and vomiting. The phytotoxic effects of deoxynivalenol on plants can be summarized as growth retardation, inhibition of seedling and green plant regeneration. Trichothecenes are now recognized as having multiple inhibitory effects on eukaryote cells, including inhibition of protein, DNA and RNA synthesis, inhibition of mitochondrial function, effects on cell division and membrane effects. In animal cells, they induce apoptosis, a programmed cell death response. Current knowledge about the eukaryotic signal transduction cascades and downstream gene products activated by trichothecenes is limited, especially in plants. In mammalian cells, certain trichothecenes trigger a ribotoxic stress response and activate mitogen-activated protein kinases. DON mediates the inflammatory response by modulating the binding activities of specific transcription factors and subsequently inducing cytokine gene expression. Several genes are up-regulated in wheat in response to trichothecene mycotoxins; the significance, if any, of these genes in the host response to trichothecenes has yet to be elucidated.

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“…In previous research, Rocha et al (2005) found three mRNA transcript fragments, over expressed in wheat roots treated with DON, with full or partial homology to retrotransposons. This and other research (Ivashuta et al 2002) implies that there may be genetic or epigenetic transposon activation in plant cells in response to stress. The present cytological results might indicate that DON as a toxicant might evoke the flower organ dysfunction by interfering with normal gene regulation responsible for meiotic chromosome division.…”

Section: Mw (Bp)mentioning

confidence: 77%

Molecular and cytological effects of deoxynivalenol mycotoxin (DON) in Triticum vulgaris using RAPD- PCR Analysis

Mahmoud¹,

Fadl-Allah

El-Tawab

et al. 2008

Chromosome Botany

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ABSTRACT. Based on the cytological and molecular studies, types and frequency of chromosomal aberrations during meiotic division of wheat plants treated with different concentrations of deoxynivalenol (DON) were investigated. The common types of abnormalities that have been recorded were stickiness, outside, laggards, bridges, fragments, unequal division, as well as multipolar and micro-nucleated cells. Regarding the first meiotic division, the percentage of abnormal cells detected from untreated plants was 2.12% however, plants treated with 5, 10, 15, 20 and 25µg/ml DON revealed 3.73, 6.62, 6.78, 9.23 and 8.18% of abnormalities, respectively. In the second meiotic division, control plants exhibit 2.69% of abnormalities while, 2.29, 4.34, 4.23, 5.59, 4.46% of abnormal cells were detected from plants treated with 5, 10, 15, 20 and 25µg/ml DON, respectively. These results demonstrate the significant effect of treatment with DON on the total percentage of irregularities during meiosis. DNA amplification patterns of wheat plants treated with DON using two RAPD primers revealed some variations in the DNA profiles within the examined samples as compared with the control. Such variations include band intensity and appearance of novel bands or disappearance of others. The polymorphism of the detected DNA fragments particularly the absence of a number of fragments in some plants treated with different concentrations of DON suggested that contaminated wheat grains with this toxin or mycotoxigenic fungi might induce DNA rearrangements, deletions and/or changes in the DNA sequence that lead to the observed changes.

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Genotype‐dependent transcriptional activation of novel repetitive elements during cold acclimation of alfalfa (Medicago sativa)

Cited by 40 publications

References 47 publications

Molecular cytogenetical effects of some mycotoxins to chromosomes of Triticum durum using RAPD-PCR fluorescence in situ hybridization

Molecular cytogenetical effects of some mycotoxins to chromosomes of Triticum durum using RAPD-PCR fluorescence in situ hybridization

Effects of trichothecene mycotoxins on eukaryotic cells: A review

Molecular and cytological effects of deoxynivalenol mycotoxin (DON) in Triticum vulgaris using RAPD- PCR Analysis

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