Genotype comparisons of strains of Candida albicans from patients with cutaneous candidiasis and vaginal candidiasis

She, Xiaodong; Wang, Xuejun; Fu, Meihua; Shen, Ya; Liu, Weida

doi:10.1097/00029330-200808010-00021

Cited by 13 publications

(9 citation statements)

References 22 publications

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“…Several published studies have reported a predominance of genotype A in clinical isolates of C. albicans [17,18]. However, the rate of genotype A that detected in this study was located within the range from 50 to 90% that reported in previous studies [19,20].…”

Section: Discussioncontrasting

confidence: 77%

Genotyping and Antifungal Vulnerability of Candida albicans Isolated from Cancer Patients in Dewaniyeh Governorate.

Shalash¹,

Al-Hamdani²

2018

IJMS

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Background: Candida infections are one of the major causes of morbidity in cancer patients. Aim: To determine the genotype and antifungal susceptibility of C. albicans isolated from cancer patients and compared with that from healthy individuals. Materials and methods: Oral swabs collected from cancer patients and healthy subjects were screened for the occurrence of C. albicans. Isolates were identified to species level by the conventional mycological methods. Genotypes were determined with use of 25S rDNA PCR analysis. Susceptibility testing was performed using HiCombo MIC technique. Results: Phenotypic examination showed that oral C. albicans was detected in 90% of cancer patients and 50% of healthy controls. PCR shows that the isolates of A, B, C and T genotypes, among which genotype A C. albicans was the predominant genotype. Genotype A C. albicans recognized the entirely isolates in healthy group. Isolates were most sensitive to amphotericin B. Occurrence of resistance to amphotericin B was the lowest followed by itraconazole. However, the isolates demonstrated a high rate of resistance to ketoconazole and fluconazole. Conclusion: Based on these results, 25S rDNA have been shown to be a useful criterion for distinguishing among various isolates of C. albicans. Amphotericin B is effective antifungal agents that can be used against isolates.

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Section: Discussioncontrasting

confidence: 77%

Genotyping and Antifungal Vulnerability of Candida albicans Isolated from Cancer Patients in Dewaniyeh Governorate.

Shalash¹,

Al-Hamdani²

2018

IJMS

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“…Barros et al found sixteen genotypes among 56 samples of C. albicans isolated from the oral cavity [4]. A comparison between this study and the previous ones [4][5]7] in terms of genotype is difficult due to the different number of strains and also to their prevalence in various clinical conditions, body sites or geographical conditions. Genotype A was predominant in all the examined groups and, surprisingly, genotype C was identified more frequently in isolates sampled from patients with HIV (see Table 1).…”

Section: Discussionmentioning

confidence: 50%

“…Genotypes A, B, and C were also detected in 73 strains sampled from dental biofilms of severe early childhood caries [5]. In another study, genotypes were detected by analysing 151 strains of C. albicans (71 samples from infant patients with cutaneous candidiasis and 61 samples from females with vaginal candidiasis), and no distinctive association was found between genotype and the site of cutaneous infection [7]. Barros et al found sixteen genotypes among 56 samples of C. albicans isolated from the oral cavity [4].…”

Section: Discussionmentioning

confidence: 98%

“…The products were analysed using DNA microfluidic chips of Experion Automated Electrophoresis System (Bio-Rad, USA). The genotypes of C. albicans can be divided into 5 groups by the size of DNA amplified fragments (450 bp for group A, 840 bp for group B, 450 and 840 bp for group C, 1080 bp for group D and 1400 bp for group E) [5][6][7]. The results were submitted to a preliminary multiple correspondence analysis (MCA) which had been assessed using XlStat-Ecology 2018.7 version.…”

Section: Methodsmentioning

confidence: 99%

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Genotype comparison of Candida albicans isolates from different clinical samples

Bostănaru

Roșca²,

Minea

et al. 2019

Revista Romana De Medicina De Laborator

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Background: Fungal infections are a health issue paradoxically fuelled by the developments in medical care. Objectives: Our study is an investigation on the correlation between the infection site and the genotypes of Candida albicans strains isolated from Romanian patients. Methods: A total number of 301 isolates from different clinical sites were investigated in terms of genotype determination. Results: The isolates were clustered in three groups according to their genotype: 55.81% showed genotype A, 14.62% genotype B, and 29.57% genotype C. Conclusions: No significant correlation was found between the genotype and the infection site, but a significant correlation was found between genotype C and isolates from HIV patients proving that C. albicans pathogenicity probably relies on factors related to the host.

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“…According to the PCR products, the genotypes of C. albicans can be classified by the size of DNA amplified into 3 groups: 450 bp for group A, 840 bp for group B, 450 and 840 bp for group C) [17][18][19].…”

Section: Albicans Genotype Determination (G) By Polymerase Chain Rmentioning

confidence: 99%

Phenotypic and genotypic variations in Candida albicans isolates from Romanian patients

Roșca¹,

Bostănaru

Minea

et al. 2018

Revista Romana De Medicina De Laborator

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Background: During the last two decades a major increase in the proportion of severe fungal infections has been noted due to the excessive use of broad-spectrum antibiotics, catheters, and a growing number of immunocompromised patients. Objectives: This is the first investigation providing complete data regarding the phenotypic and genotypic profiles of Candida albicans (C. albicans) isolates in Romanian patients. Methods: We investigated 301 isolates in terms of genotype determination (G), resistogram (R), phospholipase activity (Pl), haemolysis (Hl), proteinase activity (Pt), and biofilm formation (BF). Results: The analyzed isolates of C. albicans showed low values for Pt (61.73%), Hl (95.49%), and BF (60.71%), and did not present any Pl activity (92.23%). More than half of the investigated samples were genotype A with 450 bp (52.92%) and the majority (86.19%) were resistant to sodium selenite (A), boric acid (B), sodium periodate (D) and silver nitrate (E), but sensitive to cetrimide (-). One-way ANOVA analysis revealed significant effects of the infection site on biofilm formation (p = 0.0137) and no significant correlation was found between the genotype (A, B, C) and the infection site (p =0.449). Conclusions: Based on the obtained results it can be concluded that C. albicans isolates in Romanian patients exhibit different genotypic and phenotypic patterns, and no significant correlations between genotype and infection site could be observed.

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Genotype comparisons of strains of Candida albicans from patients with cutaneous candidiasis and vaginal candidiasis

Cited by 13 publications

References 22 publications

Genotyping and Antifungal Vulnerability of Candida albicans Isolated from Cancer Patients in Dewaniyeh Governorate.

Genotyping and Antifungal Vulnerability of Candida albicans Isolated from Cancer Patients in Dewaniyeh Governorate.

Genotype comparison of Candida albicans isolates from different clinical samples

Phenotypic and genotypic variations in Candida albicans isolates from Romanian patients

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