Genotype characterization of the Haematobia Irritans (diptera: muscidae) from Brazil, Dominican Republic and Colombia based on randomly amplified polymorphic dna (rapd) analysis

Abstract

RESUMOMoscas hematófagas são importantes parasitas em sistemas de produção animal, especialmente em condições confinamento. Haematobia irritans, a mosca-dos-chifres, é uma das espécies que mais causam problemas em sistemas de produção de bovinos, devido ao intenso estresse que impõe aos animais. H. irritans é um dos parasitas de bovinos que determinam as maiores perdas econômicas, as quais são significativas em muitas partes do mundo, incluindo a América do Sul. No presente trabalho, populações desta espécie provenientes do Brasil, Colômbia e República Dominicana foram estudadas através da análise do DNA polimórfico amplificado ao acaso (RAPD) para avaliar basicamente a variabilidade genética entre as populações. Quinze diferentes iniciadores decamétricos aleatórios foram utilizados na amplificação do DNA genômico, produzindo 196 fragmentos nas três populações de H. irritans. Entre as amostras de H. irritans, a população proveniente da Colômbia foi a que produziu o menor número de bandas polimórficas. [Caracterização genotípica de Haematobia irritans (Diptera: Muscidae) procedentes do Brasil, República Dominicana e Colômbia baseada na análise do DNA polimórfico amplificado ao acaso (RAPD)]. Revista Brasileira de Parasitologia Veterinária, v.17, n. 4, p.179-184, 2008. Embrapa Rondônia, Caixa Postal 406, Porto Velho, RO 78900-970, Brazil. E-mail: luciana@cpafro.embrapa.br. GENOTYPE CHARACTERIZATION OF THEBlood-sucking flies are important parasites in animal production systems, especially regarding confinement conditions. Haematobia irritans, the horn fly, is one of the most troublesome species within bovine production systems, due to the intense stress imposed to the animals. H. irritans is one of the parasites of cattle that cause significant economic losses in many parts of the world, including South America. In the present work, Brazilian, Colombian and Dominican Republic populations of this species were studied by Random Amplified Polymorphic DNA (RAPD) to assess basically genetic variability between populations. Fifteen different decamer random primers were employed in the genomic DNA amplification, yielding 196 fragments in the three H. irritans populations. Among H. irritans samples, that from Colombia produced the smallest numbers of polymorphic bands. This high genetic homogeneity may be ascribed to its geographic origin, which causes high isolation, low gene flow, unlike the other American populations, from Brazil and Dominican Republic. Molecular marker fragments, which its produced exclusive bands, detected in every sample enabled the population origin to be characterized, but they are also potentially useful for further approaches such as the putative origin of Brazilian, Colombian and Dominican Republic populations of horn fly from South America. Similarity indices produced by chemo metric analysis showed the closest relationships between flies from Brazil and Dominican Republic, while flies from Colombia showed the greatest genotypic differentiation relative to the others populations.KEY WORDS: Horn...

Detection of Genetic Relationship and Genetic Variation of the Four Species of Real Flies Using Molecular Markers

“…, The metal fly was characterized by the highest percentage of unique bands, reaching (24) bands, while the horse fly got the lowest percentage of unique bands, reaching (12) bands. As for the absent bands, the house fly got the least number of absent bands, it was (3) A bands Whereas the horse fly got the highest number of absent bands as it reached (6) bands, and these bands are used as a diagnostic and distinguishing characteristic for those types, so the appearance of those bands in one type indicates a mutation in a specific loci that led to the identification of the primers of this loci and the emergence of the unique bands, as well Absent bands, as a mutation occurs in the recognition site of the initiator only in one species without the other types, which leads to the cancellation of that recognition and the bands disappears and this is consistent with the results of most researchers (Sharma et al, 2015a: Brito et a., 2008: malviya et al , 2011,2012).…”

Detection of the Genetic Relationship and Genetic Fingerprint of Real Ants (Hymenoptera: Formicidae) using RAPD-PCR Markers in Iraq

“…The efficiency of the primers used in the study varied between the studied samples, and the highest efficiency was for the primer (P7), which amounted to (15.4), and the lowest efficiency was for the primer (P6), as it recorded (6.38). The discriminatory ability, while it reached (15.7), while the least discriminatory ability was recorded for the markers (P7), as it reached (6.5) (GLÁUCIA et al,2019) ( Bajpai, 2016: Brito , 2008. Estimating the genetic distance: An estimate of the genetic dimension was carried out through the results of the RAPD-PCR markers between ( 16) isolated samples from different regions of different types of real ants using the genetic program (NTSYS-PC.version 2.02i), which is based in its analyzes on the equation (Nei and Li, 1979).…”

Studies on Molecular Taxonomy and Morphological Characteristics of Haematobia irritans exigua (de Meijere, 1903); A New Range Extension in the Bargur Hills of Erode District in Tamil Nadu, India

“…The Haematobia irritans and Haematobia exigua have often been regarded as different species [30,31], or as subspecies [25,31]. There are only a few studies that have looked at the molecular characterization and variability of Haematobia species in terms of genetic surveys [32]. The genetic and morphological differences between Haematobia irritans and H. exigua and molecular phylogeny of Japanese stomoxyini flies were reported [14].…”