Genotype characterization of Haematobia irritans from different Brazilian geographic regions based on randomly amplified polymorphic DNA (RAPD) analysis
Abstract:irritans Haematobia irritans Haematobia irritans Haematobia irritans procedentes de diferentes regiões geográficas brasileiras procedentes de diferentes regiões geográficas brasileiras procedentes de diferentes regiões geográficas brasileiras procedentes de diferentes regiões geográficas brasileiras procedentes de diferentes regiões geográficas brasileiras baseada na análise do DNA polimór baseada na análise do DNA polimór baseada na análise do DNA polimór baseada na análise do DNA polimór baseada na análise d… Show more
“…The two flies have often been regarded as di#erent species (Bezzii, 1911;Malloch, 1932;Mackerras, 1933;Hennig, 1964;Van Emden, 1965;Snyder, 1965;Pont, 1986), or as subspecies (Schat, 1903;Zumpt, 1973;Pont, 1977). Genetic surveys of Haematobia species are limited to a few papers that examined the molecular characterization and variability of H. irritans populations (Castiglioni and Bicudo, 2005;Brito et al, 2007). No such reports have been published on H. exigua.…”
Molecular analysis based on mitochondrial DNA (mtDNA) sequence was performed to elucidate the genetic di#erence between Haematobia irritans (Linnaeus) and H. exigua (de Meijere), and the phylogenetic relationship among Japanese Stomoxyini species (Stomoxys calcitrans (Linnaeus), S. indicus Picard, S. uruma Shinonaga et Kano, and Haematobosca sanguinolenta (Austen) including Haematobia). Two populations obtained from Obihiro and Morioka which were morphologically identified as H. irritans were genetically identical. Two populations obtained from Taiwan and Vietnam which were morphologically identified as H. exigua were almost genetically identical, but there was a small divergence (0.3῍0.4῎) between them. Nucleotide sequence divergences between H. irritans from Obihiro-Morioka and H. exigua from Taiwan-Vietnam were 1.8῍1.9῎, and those between Haematobia and other separate species of Stomoxys and Haematobosca were 6.7῍10.9῎. In cluster analysis based on mtDNA, Haematobia species were close to Haematobosca sanguinolenta. S. calcitrans and S. uruma were closely related to each other, and S. indicus was distantly related to these two species. We discussed the status of H. irritans and H. exigua based on mtDNA, and the discrepancies of molecular phylogeny versus the previous hypotheses based on morphological criteria in Japanese Stomoxyini.
“…The two flies have often been regarded as di#erent species (Bezzii, 1911;Malloch, 1932;Mackerras, 1933;Hennig, 1964;Van Emden, 1965;Snyder, 1965;Pont, 1986), or as subspecies (Schat, 1903;Zumpt, 1973;Pont, 1977). Genetic surveys of Haematobia species are limited to a few papers that examined the molecular characterization and variability of H. irritans populations (Castiglioni and Bicudo, 2005;Brito et al, 2007). No such reports have been published on H. exigua.…”
Molecular analysis based on mitochondrial DNA (mtDNA) sequence was performed to elucidate the genetic di#erence between Haematobia irritans (Linnaeus) and H. exigua (de Meijere), and the phylogenetic relationship among Japanese Stomoxyini species (Stomoxys calcitrans (Linnaeus), S. indicus Picard, S. uruma Shinonaga et Kano, and Haematobosca sanguinolenta (Austen) including Haematobia). Two populations obtained from Obihiro and Morioka which were morphologically identified as H. irritans were genetically identical. Two populations obtained from Taiwan and Vietnam which were morphologically identified as H. exigua were almost genetically identical, but there was a small divergence (0.3῍0.4῎) between them. Nucleotide sequence divergences between H. irritans from Obihiro-Morioka and H. exigua from Taiwan-Vietnam were 1.8῍1.9῎, and those between Haematobia and other separate species of Stomoxys and Haematobosca were 6.7῍10.9῎. In cluster analysis based on mtDNA, Haematobia species were close to Haematobosca sanguinolenta. S. calcitrans and S. uruma were closely related to each other, and S. indicus was distantly related to these two species. We discussed the status of H. irritans and H. exigua based on mtDNA, and the discrepancies of molecular phylogeny versus the previous hypotheses based on morphological criteria in Japanese Stomoxyini.
“…Since insects have a large genome size with high levels of polymorphism, RAPD-PCR proves to be a cost-effective technique to unravel inter and intra-specific relationships among them [3][4][5][6]. RAPD-PCR has been substantially used to explore genetic relatedness in dipterans [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21]. In the present study, genetic relatedness among three calliphorid species, namely Hemipyrellia pulchra (Weidemann), Lucilia cuprina (Weidemann), and Chrysomya megacephala (Fabricius), by RAPD-PCR using twenty arbitrary decamer primers.…”
Molecular genetic markers have been effectively used to analyze genetic relationships and diversity among different groups of dipterans. The emergence of Polymerase Chain Reaction (PCR) facilitated analysis of molecular markers e.g., Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR), has contributed a great deal in resolving the genetic relatedness in many dipterans of medical, veterinary, and economic importance. In the present study, an attempt has been made to explore the phylogenetic relationships among three calliphorid species, namely Hemipyrellia pulchra (Weidemann) and Lucilia cuprina (Weidemann), Chrysomya megacephala (Fabricius), employing Random Amplified Polymorphic DNA - Polymerase Chain Reaction (RAPD-PCR) technique using twenty random decamer primers. Complete genomic DNA was isolated from the three species and amplified by PCR using twenty random decamer primers. A total of 285 bands ranging from 141 bp to 2648 bp were generated. Tools for population genetic analysis (TFPGA) software was used to calculate genetic identity among the three species. A close relationship among the three species is reflected by high values of mean Genetic identity (0.661–0.713).
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