Genotype-by-environment interaction of newly-developed sweet potato genotypes for storage root yield, yield-related traits and resistance to sweet potato virus disease

Ngailo, Stephan; Shimelis, Hussein; Sibiya, Julia; Mtunda, K.; Mashilo, Jacob

doi:10.1016/j.heliyon.2019.e01448

Cited by 51 publications

(44 citation statements)

References 39 publications

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“…The whole mating pairs produced many compatible pairs and many seeds or seedlings for base population breeding. This is in line with the research of Ngailo, Shimelis, Sibiya, Mtunda, & Mashilo (2019) and Rukundo, Shimelis, Laing, & Gahakwa (2017) that reported on a recombination through sexual reproduction can bring four new characters with a worthy combination of significant traits and a single seed can give rise to a potentially commercial variety.…”

Section: Seeds Numbersupporting

confidence: 87%

Crossing Among Sixteen Sweet Potato Parents for Establishing Base Populations Breeding

Lestari

Hapsari²,

Basuki³

2019

Agrivita.J.Agr.Sci

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The base-population of the controlled cross breeding is one of the important factors to develop a new improved cultivar. Since the incompatible nature of sweet potato remains a barrier for genetic improvement, therefore it requires a lot of crossed pairs. This study aimed to determine the level of incompatibility among crossing line between high yielding and micronutrient content cultivars. The field experiment conducted at Brawijaya University Research Station, Jatikerto-Malang, during February to August 2015. The North Carolina Design II was applied to sixty pairs controlled cross breeding and their sixty reciprocal pairs of six cultivars for micronutrient content enrichment with ten high yielding cultivars. The observations were made to the cross flowers number, capsules, fruit sets, and seeds number. The level of incompatibility between crossed pairs was determined by the level of fruit set. The result showed that most pairs were compatible (fruit set > 20%) and only few were incompatible (fruit set < 10%). Among six parents with micronutrient content enrichment, two of them, have a high compatibility as as female parents, to all the high yielding cultivars, i.e. BIS OP-61 and Cangkuang. Positioning as a female parent can improve the effectiveness of its selection scheme.

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Section: Seeds Numbersupporting

confidence: 87%

Crossing Among Sixteen Sweet Potato Parents for Establishing Base Populations Breeding

Lestari

Hapsari²,

Basuki³

2019

Agrivita.J.Agr.Sci

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show abstract

“…Several strategies could be applied in plant epidemiology to control plant viruses by taking into consideration virus–vector and virus–host interactions. To combat viral diseases such as sweet potato virus disease, the most common and effective strategy to date has been to select resistant sweet potato genotypes (Tairo et al , 2005; Ngailo et al , 2019). However, there are few sweet potato cultivars resistant to sweet potato virus disease in the field, especially in East Africa where there is a great demand for virus‐resistant sweet potatoes for subsistence farming.…”

Section: Discussionmentioning

confidence: 99%

Development of FRET‐based high‐throughput screening for viral RNase III inhibitors

Wang

Saarela

Poque

et al. 2020

Molecular Plant Pathology

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The class 1 ribonuclease III (RNase III) encoded by Sweet potato chlorotic stunt virus (CSR3) suppresses RNA silencing in plant cells and thereby counters the host antiviral response by cleaving host small interfering RNAs, which are indispensable components of the plant RNA interference (RNAi) pathway. The synergy between sweet potato chlorotic stunt virus and sweet potato feathery mottle virus can reduce crop yields by 90%. Inhibitors of CSR3 might prove efficacious to counter this viral threat, yet no screen has been carried out to identify such inhibitors. Here, we report a novel high-throughput screening (HTS) assay based on fluorescence resonance energy transfer (FRET) for identifying inhibitors of CSR3. For monitoring CSR3 activity via HTS, we used a small interfering RNA substrate that was labelled with a FRETcompatible dye. The optimized HTS assay yielded 109 potential inhibitors of CSR3 out of 6,620 compounds tested from different small-molecule libraries. The three best inhibitor candidates were validated with a dose-response assay. In addition, a parallel screen of the selected candidates was carried out for a similar class 1 RNase III enzyme from Escherichia coli (EcR3), and this screen yielded a different set of inhibitors. Thus, our results show that the CSR3 and EcR3 enzymes were inhibited by distinct types of molecules, indicating that this HTS assay could be widely applied in drug discovery of class 1 RNase III enzymes. K E Y W O R D Sfluorescence resonance energy transfer, high-throughput screening, RNA silencing suppressor, RNase III, small interfering RNA

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“…Vimala and Nair (1988) observed that for 20 sweetpotato cultivars, the segregation patterns of several morphological characters showed continuous and overlapping variation, indicating the quantitative nature for the morphological traits they studied. Many of the genotype · environment interactions for storage root characteristics and yield have been studied in sweetpotato, but little is known concerning environmental effects on leaf characteristics (Gr€ uneberg et al, 2005;Laurie and Booyse, 2015;Mwololo et al, 2009;Ngailo et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Phenotypic Variation in Leaf Morphology of the USDA, ARS Sweetpotato (Ipomoea batatas) Germplasm Collection

Jackson¹,

Harrison²,

Jarret³

et al. 2020

horts

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During 2012–14, 737 sweetpotato, Ipomoea batatas (L.) Lam. (Convolvulaceae), plant introduction (PI) accessions from the U.S. Department of Agriculture, Agricultural Research Service (USDA, ARS) sweetpotato germplasm collection were evaluated for several phenotypic leaf and plant characteristics, and a photographic record of each accession was made. Data were prepared for placement in the USDA, ARS Germplasm Resources Information Network (GRIN) database and the sweetpotato ontology. The parameters recorded for each genotype were canopy coverage, vine length, general leaf outline, leaf lobing, shape of the central leaf lobe, number of leaf points, leaf petiole length, leaf width, leaf length, leaf width × length, and leaf width/length (aspect ratio). The data indicate that there is wide genetic diversity for vegetative phenotypic characteristics within the USDA, ARS sweetpotato germplasm collection. This study provides important phenotype information for the USDA, ARS sweetpotato collection that has been lacking and can be used for curation of the collection and by researchers and breeders working with this important global food crop.

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Genotype-by-environment interaction of newly-developed sweet potato genotypes for storage root yield, yield-related traits and resistance to sweet potato virus disease

Cited by 51 publications

References 39 publications

Crossing Among Sixteen Sweet Potato Parents for Establishing Base Populations Breeding

Crossing Among Sixteen Sweet Potato Parents for Establishing Base Populations Breeding

Development of FRET‐based high‐throughput screening for viral RNase III inhibitors

Phenotypic Variation in Leaf Morphology of the USDA, ARS Sweetpotato (Ipomoea batatas) Germplasm Collection

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