Genotype analysis of varicella-zoster virus isolates from suburban Shanghai Municipal Province, China

Sun, Zhibo; Guo, Yue; Li, M.; Yao, Zhirong

doi:10.1099/jmm.0.000208

Cited by 5 publications

(4 citation statements)

References 20 publications

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“…In the present study, one genotype J VZV strain was detected from 26 samples with anti-VZV IgM positive. Similar to the present findings, Sun et al identified the 69 VZV strains from clinical patients between May 2011 and June 2013 in eastern China as genotype J [29]. Therefore, our results and previous findings suggested that the current varicella vaccine is suitable for regular use in China because the vaccine strain (Oka strain) belongs to genotype J.…”

Section: Discussionsupporting

confidence: 91%

Seroprevalence and molecular characteristics of varicella-zoster virus infection in Chinese children

Luan¹,

Shen²,

Qiu

et al. 2019

BMC Infect Dis

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Background Varicella-zoster virus (VZV) infection in children is an important public health problem in China. We performed the current study to explore the seroprevalence of VZV infection in Chinese children in order to provide more information for improvement of varicella vaccination in China. Methods Three thousand fourteen children were recruited from Chinese kindergarten students aged from four to six years. Anti-VZV IgG and IgM were assayed using enzyme-linked immunosorbent assay. Both ORF22 and ORF62 of VZV were amplified, sequenced, and analyzed by nested PCR. Results Among 3014 children, 43.9% of boys and 46.3% of girls were vaccinated with varicella vaccine, respectively. The seroprevalence of anti-VZV IgG was 54.4% in the children with varicella vaccination, which was significantly higher than those in unvaccinated children (49.2%) (χ 2 = 8.206, P = 0.004). Among of the vaccinated children, the detection rates of VZV IgG antibody increased with age, with 49.4, 50.9 and 58.9% in 4, 5 and 6-year groups, respectively (Trend χ 2 = 17.202, P = 0.002). However, there was no difference in anti-VZV IgG detection rates among those unvaccinated children in different age groups (Trend χ 2 = 8.681, P = 0.070). In addition, 13 boys and 13 girls were positive for anti-VZV IgM, respectively. Among of them, eight children (0.6%) have received varicella vaccination, which was similar to those in unvaccinated children (1.1%). However, only one ORF22 sequence was isolated from an unvaccinated 5-year boy. Compared to the reference VZV sequences, the nucleotide homology was estimated to be 99.7% with genotype J. Conclusions Our study indicated that about half of Chinese children aged four to six years have a high risk of VZV infection. It should be helpful for the evaluation on the necessity of varicella immunization in China.

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Section: Discussionsupporting

confidence: 91%

Seroprevalence and molecular characteristics of varicella-zoster virus infection in Chinese children

Luan¹,

Shen²,

Qiu

et al. 2019

BMC Infect Dis

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“…These results were consistent with previous observations (17). However, it was slightly different from the predominant genotype in suburban Shanghai in previous report (18).…”

Section: Discussionsupporting

confidence: 93%

Characterization and Comparison of Genetic Variation in Clinical Varicella-Zoster Virus Isolates Collected from Shanghai and Urumqi, China

Zhang

Song

et al. 2020

Jpn J Infect Dis

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Varicella-zoster virus (VZV), the cause of chickenpox and zoster, was fully sequenced and the first vaccine as the human herpesvirus. VZV is relatively genetic stable, therefore, its global surveillance clades provides essential information for VZV evolution, immigration and importation of different viral strains and recombination events. Eighty-eight VZV isolates from China (Shanghai and Urumqi) were A c c e p t e d M a n u s c r i p t genotyped using a scattered single nucleotide polymorphism (SNP) method in this prospective study. Our results were based on sequencing open reading frames (ORFs)

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“…Similar to the present findings, Sun et al identified the 69 VZV strains isolated from clinical patients between May 2011 and June 2013 in eastern China as genotype J [30].…”

Section: Discussionsupporting

confidence: 90%

Seroprevalence and molecular characteristics of varicella-zoster virus infection in Chinese children

Luan

Shen

Qiu

et al. 2019

Preprint

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Background Varicella-zoster virus (VZV) infection in children is an important public health problem in China. We performed the current study to explore the seroprevalence of VZV infection in Chinese children in order to provide more information for improvement of varicella vaccination in China. Methods 3014 serum samples were collected from Chinese kindergarten students aged from four to six years. Anti-VZV IgG and IgM were assayed using enzyme-linked immunosorbent assay. Both ORF22 and ORF62 of VZV were amplified, sequenced, and analyzed by nested PCR. Results Totally, 43.9% of boys and 46.3% of girls were vaccinated with varicella vaccine, respectively. The seroprevalence of anti-VZV IgG was 54.4% in the children with varicella vaccination, which was significantly higher than those in unvaccinated children (49.2%) (χ2=8.206, P=0.004). Among of the vaccinated children, the detection rates of VZV IgG antibody increased with age, with 49.4%, 50.9% and 58.9% in 4, 5 and 6-year groups, respectively (Trend χ2=17.202, P=0.002). However, there was no difference in anti-VZV IgG detection rates among those unvaccinated children in different age groups (Trend χ2=8.681, P=0.070). In addition, thirteen boys and 13 girls were positive for anti-VZV IgM, respectively. Among of them, eight children (0.6%) have received varicella vaccination, which was similar to those in unvaccinated children (1.1%). However, only one ORF22 sequence was isolated from an unvaccinated 5-year boy. Compared to the reference VZV sequences, the nucleotide homology was estimated to be 99.7% with genotype J. Conclusions Our study indicated that about half of Chinese children aged four to six years have a high risk of VZV infection. It should be helpful for the evaluation on the necessity of varicella immunization in China.

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Genotype analysis of varicella-zoster virus isolates from suburban Shanghai Municipal Province, China

Cited by 5 publications

References 20 publications

Seroprevalence and molecular characteristics of varicella-zoster virus infection in Chinese children

Seroprevalence and molecular characteristics of varicella-zoster virus infection in Chinese children

Characterization and Comparison of Genetic Variation in Clinical Varicella-Zoster Virus Isolates Collected from Shanghai and Urumqi, China

Seroprevalence and molecular characteristics of varicella-zoster virus infection in Chinese children

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