Genotoxicity studies on the root extract of Polygala tenuifolia Willdenow

Shin, Ki Young; Won, Beom Young; Ha, Hyun Jee; Yun, Yeo Sang; Lee, Hyung Gun

doi:10.1016/j.yrtph.2015.01.016

Cited by 12 publications

(9 citation statements)

References 25 publications

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“…TEN is the active component of the Chinese herb Polygala tenuifolia root that has no genotoxic effects and is safe at the proper dose (Shin et al 2015 ). It has been suggested as a potential drug for the treatment of osteoporosis (Yang et al 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Tenuigenin promotes the osteogenic differentiation of bone mesenchymal stem cells in vitro and in vivo

et al. 2016

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Osteoporosis, which is a systemic skeletal disease characterized by low bone mineral density and microarchitectural deterioration of bone quality, is a global and increasing public health problem. Recent studies have suggested that Tenuigenin (TEN), a class of native compounds with numerous biological activities such as anti-resorptive properties, exerts protective effects against postmenopausal bone loss. The present study aims to investigate the osteogenic effects of TEN on bone mesenchymal stem cells (BMSCs) in vitro and in vivo. Alkaline phosphatase (ALP) activity/staining, Alizarin red staining and the expression of osteogenic markers, including runt-related transcription factor 2, osterix, osteocalcin, collagen Iα1, β-catenin and glycogen synthase kinase-3β were investigated in primary femoral BMSCs from C57/BL6 mice cultured under osteogenic conditions for 2 weeks to examine the effects of TEN. An ovariectomized (OVX) mouse model was used to investigate the effect of TEN treatment for 3 months in vivo. We found that ALP activity, mineralized nodules and the expression of osteogenic markers were increased and WNT/β-catenin signaling was enhanced in vitro and in vivo. Bone parameters, including trabecular thickness, trabecular number and bone mineral density were higher in the OVX+TEN group than in control OVX mice. Our results suggest the therapeutic potential of TEN for the treatment of patients with postmenopausal osteoporosis.

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Section: Discussionmentioning

confidence: 99%

Tenuigenin promotes the osteogenic differentiation of bone mesenchymal stem cells in vitro and in vivo

et al. 2016

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“…The Ames test was developed by Ames et al in the early 1970s and is internationally recognized as the standard for determination of drug-induced genetic changes. Most genotoxic carcinogens in rodents and humans can be detected by the Ames test [46]. The Ames test is commonly used as an initial screening tool for genotoxicity, and is particularly sensitive to detection of point mutation induction activity.…”

Section: Discussionmentioning

confidence: 99%

Genotoxicity and Safety Pharmacology Studies of Indole Alkaloids Extract from Leaves of Alstonia scholaris (L.) R. Br.

Zhao

Su²,

Shang

et al. 2020

Nat. Prod. Bioprospect.

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Indole alkaloids extract (IAAS) was prepared from leaves of Alstonia scholaris (L.) R. Br., an evergreen tropical plant widely distributed throughout the world. This plant has been used historically by the Dai ethnic people of China to treat respiratory diseases. This study evaluated the genotoxicity and safety pharmacology of IAAS to support clinical use. The bacterial reverse mutation (Ames) test, in vitro mammalian chromosomal aberration test, and in vivo mammalian erythrocyte micronucleus (MN) test were performed to evaluate genotoxicity. Mice were administered IAAS (240, 480, or 960 mg/kg bw) once orally to observe adverse central nervous system effects. Furthermore, beagle dogs were administered IAAS (10, 30, 60 mg/kg bw) once via the duodenum to evaluate its effects on the cardiovascular and respiratory systems. IAAS with or without S9-induced metabolic activation showed no genotoxicity in the Ames test up to 500 μg/plate, in the mammalian chromosomal aberration test up to 710 μg/mL, or in the MN test up to 800 mg/kg bw. No abnormal neurobehavioral effects were observed in mice following treatment with up to 960 mg/kg bw of IAAS. Moreover, blood pressure, heart rate, electrocardiogram parameters, and depth and rate of breathing in anesthetized beagle dogs did not differ among the IAAS doses or from the vehicle group. These data indicated that IAAS did not induce mutagenicity, clastogenicity, or genotoxicity, and no pharmaco-toxicological effects were observed in the respiratory, cardiovascular, or central nervous systems. Our results increased understanding of safety considerations associated with IAAS, and may indicate that IAAS is a possible drug candidate. Graphic Abstract

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“…The Ames test was developed by Ames and coworkers in the early 1970s to assess the mutagenic potential and to estimate carcinogenic potential of environmental mixtures [ 33 ]. The Ames test uses strains of S. typhimurium TA100, TA98, TA1535, and TA1537 and the tryptophan auxotroph strain E. coli WP2 uvr A to detect point mutations involving substitution, addition, or deletion of one or more DNA base pairs [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

The genotoxicity of an aqueous extract of Gyejibokryeong-hwan

Lee

Seo

et al. 2018

BMC Complement Altern Med

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BackgroundGyejibokryeong-hwan (Guizhi Fuling Wan in China), a mixture of five herbal plants, is a well-known treatment for renal diseases including those associated with climacteric syndrome. However, the genotoxicity of Gyejibokryeong-hwan has not yet been well established.MethodsThe present study investigated that the genotoxicity of an aqueous extract of Gyejibokryeong-hwan (GJBRHE): an in vitro chromosomal aberration test using Chinese hamster lung cells, an in vitro bacterial reverse mutation assay (Ames test) with Salmonella typhimurium and Escherichia coli strains, and an in vivo micronucleus test using ICR mouse bone marrow.ResultsGJBRHE with or without the S9 mix showed no genotoxicity in the Ames test up to 5000 μg/plate or in the in vivo MN test up to 2000 mg/kg body weight. In contrast, the chromosomal aberration test showed that GJBRHE induced an increase in the number of chromosomal aberrations compared with the control after treatment for 6 h with 4200 μg/mL GJBRHE in the presence of the S9 mix and for 22 h with 800 μg/mL GJBRHE in the absence of the S9 mix.ConclusionsGJBRHE did not cause detectable genotoxic effects in the bacterial mutation test or the in vivo MN test, however genotoxic effect was detected in the in vitro chromosomal aberration assay. Our results suggest that GJBRHE may be associated with a low risk of carcinogenesis. Thus, further detailed experiments would be needed to clarify the compound responsible for inducing this genotoxicity of GJBRHE and to determine its mechanism.

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Genotoxicity studies on the root extract of Polygala tenuifolia Willdenow

Cited by 12 publications

References 25 publications

Tenuigenin promotes the osteogenic differentiation of bone mesenchymal stem cells in vitro and in vivo

Tenuigenin promotes the osteogenic differentiation of bone mesenchymal stem cells in vitro and in vivo

Genotoxicity and Safety Pharmacology Studies of Indole Alkaloids Extract from Leaves of Alstonia scholaris (L.) R. Br.

The genotoxicity of an aqueous extract of Gyejibokryeong-hwan

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