Genotoxicity studies on permethrin, DEET and diazinon in primary human nasal mucosal cells

Tisch, M.; Schmezer, Peter; Faulde, Michael; Groh, A; Maier, H.

doi:10.1007/s004050100406

Cited by 97 publications

(51 citation statements)

References 12 publications

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“…In another report, PM significantly increased the DNA damage in a concentration-dependent manner in healthy human lymphocytes (Undeger and Basaran 2005). Possible genotoxic effects in primary human nasal mucosal cells were also investigated by Tisch et al (2002). Their findings indicated a significant genotoxic response that was concentration dependent.…”

Section: Discussionmentioning

confidence: 94%

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Olive leaf extract modulates permethrin induced genetic and oxidative damage in rats

2012

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Permethrin is a common synthetic chemical, widely used as an insecticide in agriculture and other domestic applications. The previous reports indicated that permethrin is a highly toxic synthetic pyrethroid pesticide to human and environmental health. Therefore, the present experiment was undertaken to determine the effectiveness of olive leaf extract in modulating the permethrin induced genotoxic and oxidative damage in rats. The animals used were broadly divided into four (A, B, C and D) experimental groups. Group A rats served as control animals and received distilled water intraperitoneally (n = 5). Groups B and C rats received intraperitoneal injections of permethrin (60 mg kg -1 b.w) and olive leaf extract (500 mg kg -1 b.w), respectively. Group D rats received permethrin (60 mg kg -1 b.w) plus olive leaf extract (500 mg kg -1 b.w). Rats were orally administered their respective feed daily for 21 days. At the end of the experiment rats were anesthetized and serum and bone marrow cell samples were obtained. Genotoxic damage was assessed by micronucleus and chromosomal aberration assays. Total antioxidant capacity and total oxidant status were also measured in serum samples to assess oxidative status. Treatment of Group B with permethrin resulted in genotoxic damage and increased total oxidant status levels. Permethrin treatment also significantly decreased (P \ 0.05) total antioxidant capacity level when compared to Group A rats. Group C rats showed significant increases (P \ 0.05) in total antioxidant capacity level and no alterations in cytogenetic parameters. Moreover, simultaneous treatments with olive leaf extract significantly modulated the toxic effects of permethrin in Group D rats. It can be concluded that olive leaf extract has beneficial influences and could be able to antagonize permethrin toxicity. As a result, this investigation clearly revealed the protective role of olive leaf extract against the genetic and oxidative damage by permethrin in vivo for the first time.

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Section: Discussionmentioning

confidence: 94%

“…PM was shown to induce DNA damage on rat heart cells (Vadhana et al 2010). Furthermore, potential carcinogenicity of PM was ascertained in human nasal mucosal cells (Tisch et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Olive leaf extract modulates permethrin induced genetic and oxidative damage in rats

2012

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“…Likewise Undeğer and Başaran (2005) found that PM significantly increased DNA damage in human lymphocytes. Possible genotoxic effects in primary human nasal mucosal cells were also investigated by Tisch et al (2002). Their findings indicated a significant genotoxic response that was concentration dependent thereby they provided evidence for the potential carcinogenicity of PM to these cells.…”

Section: Discussionmentioning

confidence: 99%

“…PM was also shown to induce DNA damage on rat heart cells (Vadhana et al, 2010). Furthermore, potential carcinogenicity of PM was ascertained in human nasal mucosal cells (Tisch et al, 2002). In addition Hu et al (2010) demonstrated that PM induced oxidative stress in rat adrenal pheochromocytoma (PC12) cells.…”

Section: Introductionmentioning

confidence: 99%

Olive (Olea europaea L.) leaf extract counteracts genotoxicity and oxidative stress of permethrin in human lymphocytes

Türkez

Toğar

2011

J. Toxicol. Sci.

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-The aim of this study was to investigate the protective effects of olive leaf extract (OLE) on genotoxicity and oxidative damage in cultured human blood cells treated with permethrin (PM) in the presence of a rat liver S9 mix containing cytochrome P 450 enzymes. Anti-genotoxic activities of OLE were studied using sister chromatid exchange (SCE) and chromosome aberration (CA) tests and furthermore total antioxidant capacity (TAC) and total oxidative status (TOS) were examined to determine the oxidative damage. Our results clearly revealed that treatment with PM (200 mg/l) alone increased SCE and CA rates and TOS level, decreased TAC level in cultured human blood cells. The OLE alone at the all tested doses did not induce any significant changes in the genotoxicity endpoint. However OLE leads to increases of plasma TAC level in vitro. OLE starts showing this positive effect at 100 mg/l. The combined treatment showed significant improvements in cytogenetic and biochemical parameters tested. Moreover, this improvement was more pronounced in the group received the high dose of the OLE. It could be concluded that the ethanol extract of OLE induced its genoprotective effect via the increase in the antioxidant capacity, inhibition of oxidative stress and scavenging of free radicals.

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“…Mammalian and nonmammalian bioassays and toxicology studies have confirmed its carcinogenic potential (8,9). PM exposure leads to DNA damage in humans and experimental animals (1,10,11). It induces oxidative damage to purine bases in the heart cells (12) and produces single-and double-strand breaks in striatum cells (13).…”

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confidence: 99%

The Effects of Taurine on Permethrininduced Cytogenetic and Oxidative Damage in Cultured Human Lymphocytes

Türkez

Aydın

2012

Archives of Industrial Hygiene and Toxicology

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The Effects of Taurine on Permethrininduced Cytogenetic and Oxidative Damage in Cultured Human LymphocytesPermethrin (PM) is a common pyrethroid pesticide used to control pests in agriculture, forestry, horticulture, health care, homes, and textile industry. It is confirmed as a strong mutagen in animals and humans. Taurine (TA) is an amino acid found in mammalian tissues that protects the cell against DNA damage. In this study, we investigated whether supplementation of human lymphocyte cultures with TA (in the concentrations of 25 μg mL-1, 50 μg mL-1and 100 μg mL-1) provided any protection against PM toxicity applied in the concentration of 200 μg mL-1. Genotoxicity was assessed using the micronucleus (MN) and sister chromatid exchanges (SCE) tests. In addition, we measured the total antioxidant capacity (TAC) and total oxidative stress (TOS) levels in the plasma to determine oxidative effects. PM increased SCE and MN levels and altered TAC and TOS levels. TA alone did not affect SCE and MN levels compared to controls, regardless of the concentration applied. In addition, it increased TAC levels without changing TOS levels. Moreover, it significantly buffered the negative cytogenetic and oxidative effects induced by PM in a clear dose-dependent manner. In conclusion, this study is the first to evidence the beneficial effects of TA against PM-induced DNA and oxidative damagesin vitro.

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Genotoxicity studies on permethrin, DEET and diazinon in primary human nasal mucosal cells

Cited by 97 publications

References 12 publications

Olive leaf extract modulates permethrin induced genetic and oxidative damage in rats

Olive leaf extract modulates permethrin induced genetic and oxidative damage in rats

Olive (Olea europaea L.) leaf extract counteracts genotoxicity and oxidative stress of permethrin in human lymphocytes

The Effects of Taurine on Permethrininduced Cytogenetic and Oxidative Damage in Cultured Human Lymphocytes

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