Genotoxicity of zineb detected through the somatic and germ-line mosaic assays and the sex-linked recessive-lethal test in Drosophila melanogaster

Tripathy, N. K.; Dey, Lara-Sophie; Majhi, Bharat Bhusan; Das, C. C.

doi:10.1016/0165-1218(88)90137-1

Cited by 11 publications

(5 citation statements)

References 23 publications

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“…It has been pointed out that for certain classes of chemicals the biological response may be diminished or even masked depending on the substrate specificity and on the S-9/substrate ratio [ 171. However, conflicting data on zineb carcinogenicity have been reported [11,12]. One of the possible zineb metabolites, ethylenethiourea (ETU), which is also the most important product in EBCD metabolism, induced tumors in rat thyroid and mouse liver.…”

Section: Discussionmentioning

confidence: 99%

In vitro transforming effect of the fungicides metalaxyl and zineb

Perocco

Colacci

Bonora

et al. 1995

Teratog Carcinog Mutagen

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The cytotoxic effects and the transforming properties of two fungicides, metalaxyl and zineb, whose mutagenic or carcinogenic activity has not been clarified yet, were analyzed in the in vitro BALB/c 3T3 cell transformation test both in the presence and in the absence of an exogenous metabolizing system. Zineb was completely detoxified when the exogenous metabolizing system was added to the target cells to increase their inherent metabolic capacity. Metalaxyl induced cell transformation at any assayed dosage, i.e., 500, 250, and 50 micrograms/ml, in the presence of bioactivation, and at the highest dosage (500 micrograms/ml) in the absence of bioactivation. The transforming effect was detectable only in the level-II transformation cultures and it was likely linked to the induction of additional cell proliferation which allowed obtaining the transformation amplification in these experimental conditions.

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Section: Discussionmentioning

confidence: 99%

In vitro transforming effect of the fungicides metalaxyl and zineb

Perocco

Colacci

Bonora

et al. 1995

Teratog Carcinog Mutagen

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“…Possible genotoxic effects of zineb have been tested with regard to occupational exposure [6] and in vitro transforming properties [7]. In Drosophila melanogaster, zineb showed genotoxic effects in somatic and germ cells [8]. More recently, both in vitro monitoring of human peripheral blood lymphocytes and studies of CHO-K1 cells revealed its genotoxic effects by analyzing the frequency of biomarkers with inhibition of the mitotic activity, delay in cell cycle progression, and increase in the frequency of chromosomal aberrations and sister chromatid exchanges [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro® on meristematic root cells of Allium cepa L.

Andrioli

Soloneski

Larramendy

et al. 2012

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…Zineb exerted a high dose-related cytotoxicity in BALB/c 3T3 mouse cells in vitro, but only in the absence of an exogenous metabolizing system (Perocco et al, 1995). Trigathy et al (1988) reported zineb as a positive genotoxic agent in somatic and germ cells of Drosophila. While Chernov and Khitsenko (1969) observed an increased incidence of lung tumors after its oral administration to C57BL mice, negative results have also been reported in both mouse strains (Innes et al, 1969) and in rats (Blackwell-Smith et al, 1953;Andrianova and Alekseev, 1970).…”

Section: Introductionmentioning

confidence: 99%

Effect of the dithiocarbamate pesticide zineb and its commercial formulation azzurro. I. Genotoxic evaluation on cultured human lymphocytes exposed in vitro

Soloneski¹

2001

Mutagenesis

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The in vitro cytogenetic effects exerted by the dithiocarbamate fungicide zineb and one of its commercial formulations currently used in Argentina, azzurro, were studied in whole blood human lymphocyte cultures. The genotoxicity of the fungicides was measured by analysis of the frequency of chromosomal aberrations and sister chromatid exchanges (SCEs) and cell cycle progression assays. Both zineb and azzurro activities were tested within the range 0.1-100.0 µg/ml immediately after in vitro lymphocyte stimulation. Only concentrations of 50.0 and 100.0 µg/ml zineb and azzurro induced a significant increase in SCE frequency over control values. Furthermore, this genotoxicity appears to be correlated with its cytotoxicity, measured as cell cycle kinetics, since both a significant delay in cell cycle progression and a significant reduction in proliferative rate index were only observed in those cultures treated with these fungicide concentrations. For both chemicals, a progressive dose-related inhibition of the mitotic activity of cultures was observed when increasing the fungicide concentration. Moreover, only the mitotic activity statistically differed from control values when doses of zineb or azzurro <10 µg/ml were employed. For both fungicides the mitotic index reached the minimal value at doses of 100 µg/ml. Both products induced a significant dose-dependent increase in the number of abnormal cells, chromatid-type and chromosome-type aberrations as well as in the total number of aberrations in the 0.1-100.0 µg/ml dose range. Based on these results, the evaluation of zineb as a controversial genotoxic/nongenotoxic compound for human health should be reconsidered. Instead, we demonstrate that the fungicide induces large DNA alterations and should be considered as a clastogenic mutagen.

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Genotoxicity of zineb detected through the somatic and germ-line mosaic assays and the sex-linked recessive-lethal test in Drosophila melanogaster

Cited by 11 publications

References 23 publications

In vitro transforming effect of the fungicides metalaxyl and zineb

In vitro transforming effect of the fungicides metalaxyl and zineb

Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro® on meristematic root cells of Allium cepa L.

Effect of the dithiocarbamate pesticide zineb and its commercial formulation azzurro. I. Genotoxic evaluation on cultured human lymphocytes exposed in vitro

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