Genotoxicity of vesicular fluid and saline extract of Taenia solium metacestodes in somatic cells of Drosophila melanogaster

Abstract

Neurocysticercosis, the most common parasitic disease of the central nervous system, is caused by cysticerci of the helminth Taenia solium, which is prevalent in developing countries and is reemerging in affluent societies. This helminth is associated with brain tumors and hematological malignancies in humans. In the present study, we analyzed the genotoxicity of vesicular fluid (VF) and a saline extract (SE) of T. solium metacestodes in the Drosophila melanogaster wing somatic mutation and recombination test (SMART). Third-instar larvae derived from standard and high bioactivation crosses were treated for approximately 48 hr with 12.5, 25.0, and 50.0 microg/ml of VF and SE of T. solium metacestodes. Negative (phosphate buffered saline) and positive (10 mM urethane) controls were also included. The results showed that the two test compounds were genotoxic in both crosses of Drosophila. Nevertheless, further research is needed to determine the genotoxic potential of specific compounds present in VF and SE and their role in the development of cancer.

Assessing the impact of pollution on the Japaratuba river in Brazil using the Drosophila wing spot test

“…In contrast, the strong dependence of the genotoxicity of the positive control urethane on the cytochromes P450 overexpressed in HB flies is consistent with the findings of Frölich and Würgler [1990], Sousa et al [2003], and Silva et al [2006]. The rank order observed in MH flies from the ST cross was 1B > 2S (Table I).…”

“…These spots all are due to mutational events because recombination is suppressed in inversion-heterozygous cells with the multiply-inverted TM3 balancer chromosome [Graf et al, 1984;Guzmán-Rincón and Graf, 1995]. A comparison of the results obtained from MH and BH flies was used to quantify the mutagenic and recombinagenic potential of the test samples [Amaral et al, 2006;Silva et al, 2006]. …”

Protection by Panax ginseng C.A. Meyer against the genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster

“…This one-generation test, which is very efficient and sensitive, is based on the ability of fruit flies to metabolize certain procarcinogens to their reactive metabolites and has been used to study the genotoxicity and antigenotoxicity of various natural compounds (Idaomar et al, 2002;Laohavechvanich et al, 2006;Silva et al, 2006;Fragiorge et al, 2007;Mezzoug et al, 2007;Tellez et al, 2007;Valadares et al, 2008). The wing SMART is based on the principle that a loss of heterozygosity leads to the expression of recessive marker genes in larval imaginal disk cells, thereby yielding clones of mutant cells that can be identified as mosaic spots on the wings (Graf et al, 1984(Graf et al, , 1998.…”

Antigenotoxic effects of Mandevilla velutina (Gentianales, Apocynaceae) crude extract on cyclophosphamide-induced micronuclei in Swiss mice and urethane-induced somatic mutation and recombination in Drosophila melanogaster

“…melanogaster are versatile and sensitive in vivo eukaryotic systems for the determination of genotoxic and antigenotoxic activity of chemical compounds and complex mixtures (Graf et al, 1998;Idaomar et al, 2002;Silva et al, 2006;Fragiorge et al, 2007;Pantaleão et al, 2007;Téllez et al, 2007).…”