Genotoxicity of sub-lethal di-n-butyl phthalate (DBP) in Nile tilapia (Oreochromis niloticus) / Genotoksičnost subletalne koncentracije di-n-butil ftalata (DBP-a) u nilskoj tilapiji (Oreochromis niloticus)

Abstract

This study aimed to assess genotoxicity in Nile tilapia (Oreochromis niloticus) erythrocytes after exposure to a sub-lethal concentration of 10 mg L -1 di-n-butyl phthalate (DBP) for 24 and 96 h. The results showed that mean MN frequencies in both DBP and ethyl methane-sulfonate (EMS, positive control for MN bioassay) groups were significantly different (p<0.01) with respect to control and solvent control groups, in both exposure scenarios. When analysing nuclear abnormalities, the frequency of notched nuclei was significantly different (p<0.05) but the frequencies of other subtypes did not change. The 96-h exposure led to an increase in the mean frequencies of notched nuclei, and also caused significant differences between MN frequencies in all groups (p<0.01). Our findings indicate that sub-lethal DBP concentrations when tested in controlled laboratory conditions have genotoxic potential towards Nile tilapia. Further detailed studies should be done for the determination of the environmental risk assessment for aquatic life since DBP is a high risk contaminant of freshwater and marine ecosystems.

Acute toxicity and responses of antioxidant systems to dibutyl phthalate in neonate and adultDaphnia magna

“…The negative impacts of DBP on aquatic organisms appear to be species-dependent. The findings of the current study differ to data reported for Oreochromis niloticus exposed to DBP, where the LC 50 was 50 mg/L for 96 h of exposure (Benli, Erkmen & Erkoç, 2016). Exposure to a mixture of DBP and Cu(II) increased the toxicity in Daphnia magna by practically two orders of magnitude in Photobacterium phosphoreum (Huang, Li & Yang, 2016).…”

“…The data suggests that DBP was more toxic for neonate Daphnia magna than for adults, which was consistent with the previously reported results. The 96 h LC 50 value for DBP exposed to Oreochromis niloticus , was 50 mg/L in adults (Benli, Erkmen & Erkoç, 2016) and 11.8 mg/L in neonates (Khalil, Elhakim & EL-Murr, 2016). Exposures to DBP in aquatic organisms ranged from 24 to 96 h and comprised a relatively wide range of LC 50 values (10 2 –10 5 μg/L).…”

Peer Review #2 of "Acute toxicity and responses of antioxidant systems to dibutyl phthalate in neonate and adult Daphnia magna (v0.1)"

“…The negative impacts of DBP on aquatic organisms appear to be species-dependent. The findings of the current study differ to data reported for O. niloticus exposed to DBP, where the LC50 was 50 mg/L for 96 h of exposure (Benli et al 2016). Exposure to a mixture of DBP and Cu(II) increased the toxicity in D. magna by practically two orders of magnitude in P. phosphoreu (Huang et al 2016).…”

“…The data suggests that DBP was more toxic for neonate D. magna than for adults, which was consistent with the previously reported results. The 96 h LC50 value for DBP exposed to O. niloticus, was 50 mg/L in adults (Benli et al 2016) and 11.8 mg/L in neonates (Khalil et al 2016). Exposures to DBP in aquatic organisms ranged from 24 to 96 h and comprised a relatively wide range of LC50 values (10 2 to 10 5 μg/L).…”

Characterization of Genotoxic Activity of Phthalates and Their Metabolites by the UmuC <i>in vitro</i> Assay

“…Humans can be exposed to phthalates in various ways (food, inhalation and dermal way) [11] but health effects (especially genotoxicity) caused by phthalates and its monoester metabolites are still not clearly understood. Literature mostly focuses on genotoxic effects of DEHP and MEHP [7,12,13] which is considered as metabolite with the highest genotoxicity. But not only MEHP is present in urine samples collected from people exposed to DEHP.…”