Genotoxicity of diuron and glyphosate in oyster spermatozoa and embryos

Akcha, Farida; Spagnol, Charlene; Rouxel, Julien

doi:10.1016/j.aquatox.2011.10.018

Cited by 109 publications

(84 citation statements)

References 50 publications

(50 reference statements)

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“…Also, no morphological changes (FSC: relative size, SSC: relative complexity) were observed upon A. catenella exposure (data not shown). Sperm viability has been widely used in mammalian sperm toxicity tests (Lewis and Ford 2012), but has only been tested in a few aquatic invertebrate species (Favret et al 2010;Akcha et al 2012). In this study, viability of sperm from oysters exposed to A. catenella was not affected.…”

Section: Viabilitymentioning

confidence: 54%

“…Because bivalves spawn their gametes into the surrounding water, reproductive output could be directly affected by different environmental pollutants (Favret et al 2010;Akcha et al 2012) or HABs (Erard-Le Denn et al 1990; Leverone et al 2006;Basti et al 2011) found there. Cellular parameters of gametes can therefore be used as bio-indicators of modification and disturbance of the environment (Akcha et al 2012). Alterations induced by noxious compounds have been observed in oyster gametes (Nice 2005).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Impact of the toxic dinoflagellateAlexandrium catenellaon Pacific oyster reproductive output: application of flow cytometry assays on spermatozoa

Goïc

Hégaret

Fabioux

et al. 2013

Aquat. Living Resour.

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-The toxic dinoflagellate Alexandrium catenella recurrently blooms on the coasts of France and produces Paralytic Shellfish Toxins (PSTs) that accumulate in bivalves. These toxins can affect various physiological functions including reproduction. The present study aims to validate measurements of sperm viability, DNA content and mitochondrial membrane potential in Pacific oyster Crassostrea gigas using flow cytometry coupled with fluorescent markers, and to use these measurements to assess the cellular parameters of sperm from Pacific oysters exposed to A. catenella. These parameters may influence fertilization, embryogenesis and larval development in free-spawning shellfish. Sperm viability and DNA content estimation were assessed using SYBR-14, which only penetrates cells with intact membranes. Cell mortality was measured with propidium iodide (PI), which penetrates cells with membrane damage. Mitochondrial membrane potential, used as an estimate of mitochondrial function, was measured using JC-1 dye, which selectively enters into mitochondria and reversibly changes colour from green to orange as the membrane potential increases. To assess the effect of toxic algae on oyster sperm, broodstock (ripe oysters) were fed toxic (A. catenella) or non toxic (Heterocapsa triquetra) dinoflagellates at 250 cell ml −1 for 9 days. After this exposure period, mature oysters were stripped and cellular responses of sperm analysed. Average DNA staining, as measured by SYBR-14, appeared lower and more variable in gametes from A. catenella-exposed oysters than in those from control oysters fed H. triquetra. Additionally, mitochondrial membrane potential of sperm from A. catenella-exposed oysters was significantly higher (1.5 fold) than that of sperm produced by oysters fed H. triquetra. Both the increase of mitochondrial membrane potential and the modification of DNA structure can be expected to impact spermatozoa ability to fertilize oocytes and could thus impact related reproductive processes.

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Section: Viabilitymentioning

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Impact of the toxic dinoflagellateAlexandrium catenellaon Pacific oyster reproductive output: application of flow cytometry assays on spermatozoa

Goïc

Hégaret

Fabioux

et al. 2013

Aquat. Living Resour.

View full text Add to dashboard Cite

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“…In another study with oyster the relative expression of seven immune-related genes was an equally sensitive endpoint to study the effects of diuron on mollusc (Luna-Acosta et al 2012). Even more sensitive was the study design set up by Akcha et al (2012) where embryo-larval development of oysters were significantly affected by diuron at a concentration of 0.05 µg/l. USEPA has published methods for measuring larval development in oyster (Chapman et.…”

Section: Comparison With Oecd Cf Methodsmentioning

confidence: 99%

“…Diuron has reproductive effects on oyster at a concentration of 0.5 µg/l (Akcha et al 2012). These were genotoxic effects on spermatozoa and not necessary regarded as mediated by endocrine mechanisms.…”

Section: Non-mammalian Toxicologymentioning

confidence: 99%

Suspected endocrine disrupting substances

Publicering¹

2013

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“…In vivo, oysters exposed to toxic algae revealed alteration of spermatozoa quality (21,25). Gametes of C. gigas are released into the surrounding seawater where they are exposed to various environmental stressors (26,27) and possibly toxic algae (10,(28)(29)(30). Blooms of A. minutum occur during summer, which is concomitant with the oyster spawning period.…”

mentioning

confidence: 99%

Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure

et al. 2014

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The Pacific oyster Crassostrea gigas accounts for a large part of shellfish aquaculture production worldwide. Aspects of morphological and functional characteristics of oyster oocytes remain poorly documented, and traditional techniques, such as microscopic observations of shape or fertilization rate, are time and space consuming. The purpose of this study was to assess for the first time viability and reactive oxygen species (ROS) production of Pacific oyster oocytes using flow cytometry (FCM) and to apply this method to determine oocyte responses to in vitro exposure to the toxic dinoflagellate Alexandrium minutum. A culture of A. minutum caused a significant increase in oocyte ROS production, which gradually increased with the age of the culture, but viability was not affected. Effect of the supernatant of the same A. minutum culture did not cause any significant modifications of oocyte morphology, viability, or ROS level. This study confirmed that some oocyte cellular characteristics can be assessed using FCM techniques. V C 2014 International Society for Advancement of Cytometry Key terms Pacific oyster; gamete quality; ROS production; viability; flow cytometry; ecotoxicology THE Pacific oyster Crassostrea gigas has considerable commercial value worldwide, including in France. Oyster seed production relies upon gamete quality, fertilization success, and larval development. As gamete quality is the first step in the spatproduction sequence, it is necessary to assess the quality of gametes in broodstock oysters first and foremost.To date, bioassays to assess oocyte quality are limited, and those available are tedious and time-consuming. Oocyte quality can be described using morphological characteristics by microscopy (1). Although microscope observations are unavoidable and useful to validate flow cytometry (FCM), assays based on microscope observations often have little predictive value and can be subjective according to each observer. Determinations of biochemical content (proteins, carbohydrates, and lipids) have been used as criteria of oocyte quality (2-4). More recently, proteomic profiles of oocytes were proposed as quality estimators (5,6). All of these biochemical assays, however, are fairly tedious to perform and provide only retrospective information. Fertilization rate, hatching rate, and larval development are used as complementary end points to evaluate oocyte quality (7-10); however, as these processes depend also upon spermatozoa quality, variability and inconsistency from one experiment to another often are encountered.

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Genotoxicity of diuron and glyphosate in oyster spermatozoa and embryos

Cited by 109 publications

References 50 publications

Impact of the toxic dinoflagellateAlexandrium catenellaon Pacific oyster reproductive output: application of flow cytometry assays on spermatozoa

Impact of the toxic dinoflagellateAlexandrium catenellaon Pacific oyster reproductive output: application of flow cytometry assays on spermatozoa

Suspected endocrine disrupting substances

Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure

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