Genotoxicity of contaminated soil and shallow well water detected by plant bioassays

Maria, Kong; Ma, Te­-Hsiu

doi:10.1016/s0027-5107(99)00072-x

Cited by 59 publications

(21 citation statements)

References 23 publications

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“…Epidemiological evidence has raised concerns about chronic atrazine exposure being a possible cause of a variety of adverse human health effects [GarajVrhovac and Zeljezic, 2002]. Hence, a thorough study of the toxicological potential of this compound is of fundamental importance [Kong and Ma, 1999] in order to design appropriate safety measures for occupationally exposed workers, while monitoring is important to minimize or prevent chronic, lowdose human exposure. Ground and surface water compartments remain a common source of potential exposure and the impact of atrazine on ecosystem should also be considered.…”

Section: Resultsmentioning

confidence: 99%

Clastogenicity of atrazine assessed with the Allium cepa test

Bolle

Mastrangelo

Tucci

et al. 2004

Environ and Mol Mutagen

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Atrazine is classified as a restricted use pesticide and it is currently included in an international revision program for re-evaluating the human and ecological (non-human populations) health risks associated with its release into the environment. The present study was undertaken to add new data on the genotoxic potential of atrazine using the Allium cepa chromosome aberration test. The test concentrations were based on the Maximum Contaminant Levels in water intended for human consumption set by European and US regulations. Atrazine produced a concentration-related increase in the number of total somatic chromosome aberrations, although this increase was statistically significant (p<0.05) only at the highest test concentration (5 microg/L). Analysis of the categories of structural chromosome damage indicated that breaks were the predominant lesion induced; the percent of cells per bulb with breaks also increased in a concentration-related manner, and the increase was statistically significant at the two highest test concentrations (1 and 5 microg/L) (p<0.05). The Allium cepa plant assay detected the clastogenicity of atrazine at concentrations that are likely to be encountered in water, a common site of atrazine contamination.

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Section: Resultsmentioning

confidence: 99%

Clastogenicity of atrazine assessed with the Allium cepa test

Bolle

Mastrangelo

Tucci

et al. 2004

Environ and Mol Mutagen

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“…Les chromosomes vagabonds montrent que la mitose est faible ce qui entraîne des risques d'aneuploïdie tandis que les chromosomes visqueux indiquent une forte toxicité ayant d'effets irréversibles qui vraisemblablement, est le principal auteur de la mort des cellules (Fiskesjo, 1985(Fiskesjo, et 1988. Selon Kong et Ma (1999) l'hypothèse est que la viscosité des chromosomes est la cause de la séparation incomplète des chromosomes fils résultants du cross-linquage des protéines chromosomiques (Samuel et al, 2010). Le nombre des aberrations mitotiques cellulaires provoquées par les concentrations d'eau des deux sites est différent de celui obtenu au niveau du control puis notons qu'aucune aberration chromosomique n'a été observée au niveau des cellules des racines d'oignons mis en culture dans le control (0%).…”

Section: Discussionunclassified

Évaluation de la cytogénotoxicité des sites de pollution du PONT et d’ACCRON de la lagune de Porto- Novo

Cakpo¹,

Sagbo²,

Mama³

et al. 2015

J. App. Bioscience.

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RÉSUMÉObjectif : Les effets cytogénotoxiques des sites du PONT et d'ACCRON de la lagune de Porto-Novo à des concentrations de 0%, 25%, 50%, 75%, 100% ont été évalués par le test de toxicité basé sur les cellules des racines des oignons. Méthodologie et résultats : Dans cette étude, la longueur des racines et le test sur les aberrations chromosomiques ont été utilisés pour déterminer la concentration efficace (96h, CE50), l'inhibition de la croissance des racines, l'indice mitotique et le taux d'aberration chromosomique. D'après les résultats obtenus, les eaux du site du PONT sont 2,24 fois plus toxiques que celles des eaux du site d'ACCRON et on a une différente significative entre la moyenne des longueurs des racines des oignons exposés dans les différentes concentrations des deux sites. Cela indique que l'inhibition de la croissance des racines dépend des concentrations. L'indice mitotique (IM) croit avec l'augmentation des concentrations des eaux sur le site d'ACCRON par contre sur le site du PONT, il diminue de 0% à 25% et croît de 50% à 100%. Les eaux des deux sites ont provoqué des aberrations chromosomiques des cellules des racines des oignons avec des malformations chromosomiques telles que les chromosomes en fragments de pont, vagabonds et collants. Les aberrations communes observées lorsque les concentrations sont faibles, sont les chromosomes en fragments de pont, visqueux, vagabonds et les anaphases polaires. Le test de génotoxicité effectué sur les chromosomes des racines des oignons a permis de mesurer les effets génotoxiques des eaux des deux sites étudiés. Conclusion et application des résultats : Les tests de cytogénotoxicité basés sur les racines d'oignons effectués sur les eaux des deux sites ont montré d'une part que les deux sites sont génotoxiques et que le site du PONT est plus toxique que celui d'ACCRON d'autre part. Mots Clés : Cytogénotoxicité, oignons, pollution, aberrations chromosomiques, inhibition de la croissance des racines. Journal of Applied Biosciences 87:8076-8084 ISSN 1997-5902Cakpo et al. J. Appl. Biosci. Évaluation de la cytogénotoxicité des sites de pollution du PONT et d'ACCRON de la lagune de Porto-Novo 8077ABSTRACT Objective: The effects cytogenotoxicity at the sites of PONT and ACCRON of Porto-Novo lagoon were evaluated using root tip cells of Allium cepa. Methodology and results: In this study, root length and chromosomal aberration assays were used to determine the 96 h effective concentration (96h, EC 50 ) roots growth inhibition, mitotic index and chromosome aberration rate. According to the results obtained, water of the site of the PONT was 2.24 times more toxic than the water of ACCRON site and one has different significant between the average lengths of the roots of onions exposed in the various concentrations of the two sites. This indicated that the root growth inhibition was concentration dependent. The mitotic index (MI) increased with increasing concentrations of ACCRON site on the other hand on the PONT site; it decreased by 0% to 25% and grew from 50% t...

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“…Gomez-Arroyo et al [34] similarly reported that well water contaminated by arsenic from Zimapan, Hidalgo town in Mexico induced sister chromatid exchange in treated Vicia faba root tips. Kong and Ma [35] reported that shallow well water collected from the vicinity of five pesticide farms induced chromosome aberration and micronucleated cells in Allium root anaphase aberration, Tradescantia-micronucleus and Tradescantia stamen hair mutation.…”

Section: Discussionmentioning

confidence: 99%

In Vivo Cytogenotoxicity and Oxidative Stress Induced by Electronic Waste Leachate and Contaminated Well Water

et al. 2013

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Environmental, plant and animal exposure to hazardous substances from electronic wastes (e-wastes) in Nigeria is increasing. In this study, the potential cytogenotoxicity of e-wastes leachate and contaminated well water samples obtained from Alaba International Electronic Market in Lagos, Nigeria, using induction of chromosome and root growth anomalies in Allium cepa, and micronucleus (MN) in peripheral erythrocytes of Clarias gariepinus, was evaluated. The possible cause of DNA damage via the assessments of liver malondialdehyde (MDA), catalase (CAT), reduced glutathione (GSH) and superoxide dismutase (SOD) as indicators of oxidative stress in mice was also investigated. There was significant (p < 0.05) inhibition of root growth and mitosis in A. cepa. Cytological aberrations such as spindle disturbance, C-mitosis and binucleated cells, and morphological alterations like tumor and twisting roots were also induced. There was concentration-dependent, significant (p < 0.05) induction of micronucleated erythrocytes and nuclear abnormalities such as blebbed nuclei and binucleated erythrocytes in C. gariepinus. A significant increase (p < 0.001) in CAT, GSH and MDA with concomitant decrease in SOD concentrations were observed in the treated mice. Pb, As, Cu, Cr, and Cd analyzed in the tested samples contributed significantly to these observations. This shows that the well water samples and leachate contained substances capable of inducing somatic OPEN ACCESSChallenges 2013, 4 170 mutation and oxidative stress in living cells; and this is of health importance in countries with risk of e-wastes exposure.

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Genotoxicity of contaminated soil and shallow well water detected by plant bioassays

Cited by 59 publications

References 23 publications

Clastogenicity of atrazine assessed with the Allium cepa test

Clastogenicity of atrazine assessed with the Allium cepa test

Évaluation de la cytogénotoxicité des sites de pollution du PONT et d’ACCRON de la lagune de Porto- Novo

In Vivo Cytogenotoxicity and Oxidative Stress Induced by Electronic Waste Leachate and Contaminated Well Water

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