Genotoxicity evaluation of electromagnetic fields generated by 835-MHz mobile phone frequency band

Abstract

It is still unclear whether the exposure to electromagnetic fields (EMFs) generated by mobile phone radiation is directly linked to cancer. We examined the biological effects of an EMF at 835 MHz, the most widely used communication frequency band in Korean CDMA mobile phone networks, on bacterial reverse mutation (Ames assay) and DNA stability (in vitro DNA degradation). In the Ames assay, tester strains alone or combined with positive mutagen were applied in an artificial mobile phone frequency EMF generator with continuous waveform at a specific absorption rate (SAR) of 4 W/kg for 48 h. In the presence of the 835-MHz EMF radiation, incubation with positive mutagen 4-nitroquinoline-1-oxide and cumene hydroxide further increased the mutation rate in Escherichia coli WP2 and TA102, respectively, while the contrary results in Salmonella typhimurium TA98 and TA1535 treated with 4-nitroquinoline-1-oxide and sodium azide, respectively, were shown as antimutagenic. However, these mutagenic or co-mutagenic effects of 835-MHz radiation were not significantly repeated in other relevant strains with same mutation type. In the DNA degradation test, the exposure to 835-MHz EMF did not change the rate of degradation observed using plasmid pBluescript SK(+) as an indicator. Thus, we suggest that 835-MHz EMF under the conditions of our study neither affected the reverse mutation frequency nor accelerated DNA degradation in vitro.

Mobile phone electromagnetic radiation activates MAPK signaling and regulates viability in Drosophila

“…One hundred three-day-old adult female flies were located in the 100 mm Petri dish with a wet filter paper to maintain humidity and exposed in the Wave Exposure V10 device (Patent No.0311892, Republic of Korea) [Chang et al, 2005]. This device produced 835 MHz SAR 1.6 W/kg or 4 W/kg EMF based on the transverse electromagnetic wave theory (Fig.…”

“…Ames test and DNA stability assay show that 835 MHz electromagnetic field (EMF) does not increase the mutation frequency or DNA degradations [Chang et al, 2005]. In Caenorhabditis elegans, 750 MHz electromagnetic radiation activates the hsp16 > GFP transgene in which the GFP reporter gene is controlled by the hsp16 promoter [de Pomerai et al, 2000].…”

“…1A) [Chang et al, 2005] at two conditions: SAR 1.6 W/kg, the proposed exposure limit by ANSI, and strong SAR 4.0 W/kg. We measured the viability, examined the induction of stress response, and tested the activation of MAPK signaling and subsequent survival/death signaling.…”

Adverse Effect of Mobile Phone on Tp53, Brcai Genes and Dna Fragmentation in Albino Rat Liver

“…In contrary, other studies failed to prove any contribution of EMR on cellular DNA damage. Hook, et al [44] found that there was not any significant effect of EMR on T lymphoblastoid cells, EMR neither affected the reverse mutation frequency nor accelerated DNA degradation [45]. Others had previously proved that 2450 MHz irradiation does not appear to cause DNA damage in cultured mammalian cells [46].…”

What’s New Among Cancer Etiology Horizon?

“…The scientific evidence shows that mobile phones emit electromagnetic radiation (radiofrequency, RF) that is essentially non-ionizing. (frequencies between 300 MHz and 300 GHZ) [56][57][58]. The specific absorption rate (SAR) measures the energy dose that subjects exposed to RF absorb and is expressed in power (watts) by tissue mass (kilograms) [W/kg].…”