Genotoxicity evaluation of a Phragmitis rhizoma extract using a standard battery of in vitro and in vivo assays

Kim, No Soo; Shin, Sarah J.; Shin, Geon-Gook; Bang, Ok‐Sun

doi:10.1016/j.jep.2019.112025

Cited by 8 publications

(5 citation statements)

References 37 publications

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“…The micronucleus assay is widely used in bone marrow cells and, to a lesser extent in peripheral blood cells ( 53 , 54 ). Therefore, we also examined the frequency of micronuclei formation in whole blood cells among young mice.…”

Section: Discussionmentioning

confidence: 99%

Genoprotective activity of the Pleurotus eryngii mushrooms following their in vitro and in vivo fermentation by fecal microbiota

et al. 2022

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Pleurotus eryngii mushrooms are commercially cultivated and widely consumed due to their organoleptic properties, and the low caloric and high nutritional value. In addition, they contain various biologically active and health-promoting compounds; very recently, their genoprotective effect in Caco-2 cells after their fermentation by the human fecal microbiota was also documented. In the current study, the effect of P. eryngii pre- and post-fermentation supernatants in micronuclei formation was evaluated in human lymphocytes. In addition, the genoprotective properties of increasing concentrations of aqueous extracts from P. eryngii mushrooms (150, 300, 600 mg/kg) against the cyclophosphamide-induced DNA damage were studied in young and elderly female and male mice in bone marrow and whole blood cells. The ability of the highest dose (600 mg/kg) to regulate the main cellular signaling pathways was also evaluated in gut and liver tissues of female animals by quantifying the mRNA expression of NrF2, Nfkβ, DNMT1, and IL-22 genes. P. eryngii post-fermentation, but not pre-fermentation, supernatants were able to protect human lymphocytes from the mitomycin C-induced DNA damage in a dose-dependent manner. Similarly, genoprotection was also observed in bone marrow cells of mice treated by gavage with P. eryngii extract. The effect was observed in all the experimental groups of mice (young and elderly, male and female) and was more potent in young female mice. Overexpression of all genes examined was observed in both tissues, mainly among the elderly animals. In conclusion, P. eryngii mushrooms were shown to maintain genome integrity through protecting cells from genotoxic insults. These beneficial effects can be attributed to their antioxidant and immunomodulatory properties, as well as their ability to regulate the cell’s epigenetic mechanisms and maintain cell homeostasis.

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Section: Discussionmentioning

confidence: 99%

Genoprotective activity of the Pleurotus eryngii mushrooms following their in vitro and in vivo fermentation by fecal microbiota

et al. 2022

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“…Phragmitis Rhizoma (Lugen in Chinese) is the fresh or dried rhizome of Phragmites communis Trin 310 . The chemical constituents of Phragmitis Rhizoma included polysaccharides, flavonoids, steroids, anthraquinones, alkaloids, volatile components, small molecules, and other components 311 .…”

Section: Tcms For the Treatment Of Covid-19 Infectionsmentioning

confidence: 99%

Potential herb–drug interactions between anti-COVID-19 drugs and traditional Chinese medicine

Ye¹,

Fan²,

Zhao³

et al. 2023

Acta Pharmaceutica Sinica B

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“…갈대(Phragmites communis)는 우리나라를 비롯한 동아시아의 온 대 지역의 습지에서 잘 자라는 수변 식물로 예부터 갈대 뿌리 는 민간에서 발열과 구토 완화 및 이뇨 촉진에 사용되어왔다 [1]. 갈대 뿌리의 물 추출물은 유전독성이 없고 돌연변이를 유 발하지 않는 것으로 나타나 [2], 안전한 식품 소재로의 활용가능 성이 있는 것으로 알려져 있다 [3]. 갈대 뿌리 추출물은 항염증, 항균 및 간세포 보호 효과를 가진 것으로 보고되었고 [4][5][6], 갈 대 뿌리의 다당체는 동물실험에서 아토피성 피부염을 완화시켰 다 [7].…”

Section: 서 론unclassified

Nitrite scavenging and lipid peroxidation inhibitory effects of solvent fractions fromPhragmites communisrhizome extract

Kim

2022

JABC

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Phragmites communis rhizome (Phragmitis rhizoma) was extracted with 70% ethanol, and then the extract was fractionated sequentially using n-hexane, chloroform, and ethyl acetate as solvents. Among the solvent fractions of Phragmitis rhizoma extract, the polyphenol content in ethyl acetate fraction was the highest. The chloroform and ethyl acetate fractions possessed a good nitrite scavenging activity. In addition, each solvent fraction showed an effective lipid peroxidation inhibitory ability at a concentration of 10 mg/mL.

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Genotoxicity evaluation of a Phragmitis rhizoma extract using a standard battery of in vitro and in vivo assays

Cited by 8 publications

References 37 publications

Genoprotective activity of the Pleurotus eryngii mushrooms following their in vitro and in vivo fermentation by fecal microbiota

Genoprotective activity of the Pleurotus eryngii mushrooms following their in vitro and in vivo fermentation by fecal microbiota

Potential herb–drug interactions between anti-COVID-19 drugs and traditional Chinese medicine

Nitrite scavenging and lipid peroxidation inhibitory effects of solvent fractions fromPhragmites communisrhizome extract

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