Genotoxicity assessment of particulate matter emitted from heavy-duty diesel-powered vehicles using the in vivo Vicia faba L. micronucleus test

Corrêa, Albertina X.R.; Cotelle, Sylvie; Millet, Maurice; Somensi, Cléder A.; Wagner, Theodoro Marcel; Radetski, Claudemir M.

doi:10.1016/j.ecoenv.2016.01.026

Cited by 28 publications

(12 citation statements)

References 31 publications

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“…The significant difference of the micronuclei frequency between the control and cells exposed to NaCl indicates the sensitivity and the reliability of this test system. Such sensitivity of V. faba roots was also caused by a wide range of other stress conditions such as heavy metal treatments (Souguir et al, 2008(Souguir et al, , 2011Yi et al, 2010), olive mill waste water (El Hajjouji et al, 2007), pesticides (Sta et al, 2012), and diesel exhaust particulate matter (Corrˆea et al, 2016). Recently, an interlaboratory study of seven laboratories from three countries, investigating the robustness of this test for soil or soil materials assessment, confirmed its applicability based on the international ISO 29200 protocol (Cotelle et al, 2015).…”

Section: Discussionmentioning

confidence: 88%

“…Several studies show that the micronucleus assay is an effective technique to assess genotoxic damage caused by toxic substances. The rate of the micronucleus is usually used to assess the genotoxicity of contaminated soil, sediment, organic material, water, and industrial effluent (Corrˆea et al, 2016;Cotelle et al, 2015;Renjana et al, 2013;Sta et al, 2012). The most frequently used plant species to test both clastogenic and aneugenic effects is Vicia faba.…”

Section: Introductionmentioning

confidence: 99%

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Chromosomal and Nuclear Alterations in the Root Tip Cells of Vicia faba Induced by Sodium Chloride

Souguir

Abd‐Alla

Hörmann

et al. 2018

Water Environment Research

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Despite the well-known toxic effect of chloride sodium (NaCl), only few studies analyze its genotoxic effect on crops. Micronucleus assay is an efficient tool for the measure of chromosomal DNA damages. Based on this test, the effects of salt on micronucleus induction, and on chromosome and nucleus changes, were investigated in Vicia faba root tips treated with NaCl (electrical conductivity = 25 dS/m) during 24 hours. V. faba meristematic cells showed a significant increase in the number of micronuclei compared with the control. Micronucleus size varied from small to large and appeared at different stages of mitosis. Moreover, different chromosomal aberrations like breaks, bridges, rings, vagrant, and lagging chromosomes, were observed in the metaphase, the anaphase, and the telophase. In addition, nuclear alterations in the interphase were characterized by the presence of binucleated cells, strap nuclei with lesions, hyperchromasia, buds, and nuclei with irregular shapes and lesions.

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Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Chromosomal and Nuclear Alterations in the Root Tip Cells of Vicia faba Induced by Sodium Chloride

Souguir

Abd‐Alla

Hörmann

et al. 2018

Water Environment Research

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“…It was further proved that metal ions enhance the ROS formation capacity of ultrafine black particles both in vivo and in vitro (127). In has now been clear that metal ions present in the DEP are responsible for its genotoxic effects (128). A recent study described the estrogenic activity of soot is not related to the metal ion concentrations present in it (129).…”

Section: The Pathological Mechanisms Of Cancer Due To Soot and Cbsmentioning

confidence: 99%

The Toxicological Mechanisms of Environmental Soot (Black Carbon) and Carbon Black: Focus on Oxidative Stress and Inflammatory Pathways

2017

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The environmental soot and carbon blacks (CBs) cause many diseases in humans, but their underlying mechanisms of toxicity are still poorly understood. Both are formed after the incomplete combustion of hydrocarbons but differ in their constituents and percent carbon contents. For the first time, “Sir Percival Pott” described soot as a carcinogen, which was subsequently confirmed by many others. The existing data suggest three main types of diseases due to soot and CB exposures: cancer, respiratory diseases, and cardiovascular dysfunctions. Experimental models revealed the involvement of oxidative stress, DNA methylation, formation of DNA adducts, and Aryl hydrocarbon receptor activation as the key mechanisms of soot- and CB-induced cancers. Metals including Si, Fe, Mn, Ti, and Co in soot also contribute in the reactive oxygen species (ROS)-mediated DNA damage. Mechanistically, ROS-induced DNA damage is further enhanced by eosinophils and neutrophils via halide (Cl− and Br−) dependent DNA adducts formation. The activation of pulmonary dendritic cells, T helper type 2 cells, and mast cells is crucial mediators in the pathology of soot- or CB-induced respiratory disease. Polyunsaturated fatty acids (PUFAs) were also found to modulate T cells functions in respiratory diseases. Particularly, telomerase reverse transcriptase was found to play the critical role in soot- and CB-induced cardiovascular dysfunctions. In this review, we propose integrated mechanisms of soot- and CB-induced toxicity emphasizing the role of inflammatory mediators and oxidative stress. We also suggest use of antioxidants and PUFAs as protective strategies against soot- and CB-induced disorders.

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“…Research on Ficus religiosa, F. benghalensis, Mangifera indica, Dalbergia sissoo, Psidium guajava and Dendrocalamus strictus explored the seasonal variation trend of particle deposition on leaves, and found that an increase in atmospheric particles that settled on leaves of selected tree species led to a reduction in chlorophyll content and an increase in ascorbic acid [14]. Investigation of the effects of particles emitted from heavy-duty diesel engines showed that exposure to the soot suspension reduced the cellular mitosis capacity in root tips of broad bean [15]. Through research on leaf structures of mangrove forest around iron mining areas in Brazil as well as the chemical component and biological accumulation of their retentates, Arrivabene et al found that iron particles in the atmospheric environment had slight effects on the stomata of mangrove leaves and the salinity balance in leaf cells.…”

Section: Introductionmentioning

confidence: 99%

Retention of Atmospheric Particles by Local Plant Leaves in the Mount Wutai Scenic Area, China

et al. 2016

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Abstract:To evaluate the characteristics of atmospheric particle retention by plant leaves during the tourism season in Buddhism-based scenic areas, plants distributed in the core area of the Mount Wutai scenic area were selected for study: Populus davidiana (Po. davidiana), Rosa hugonis Hemsl. (R. hugonis), Betula platyphylla Suk. (B. platyphylla), Rosa xanthina Lindl. (R. xanthina), Periploca sepium Bunge (Pe. sepium), Spiraea salicifolia L. (S. salicifolia), Vitex negundo var. Heterophylla (V. negundo var. heterophylla) and Pinus tabuliformis Carrière (Pi. tabuliformis). Before rain, the atmospheric suspended particle-retaining weight of the plant leaves varied in the range of 6.95˘1.55 (Pi. tabuliformis) to 38.60˘18.32 mg/cm 2 (Po. davidiana); the light shaded areas caused by particles on leaves were in the range of 7.25˘0.04 (Pi. tabuliformis) to 126.50˘6.66 cm 2 /leaf (Po. davidiana); and the atmospheric particle-retaining horizontal density of leaves varied in the range of 110˘2 (Pi. tabuliformis) to 255˘11 per cm 2 (Po. davidiana). After rain, the atmospheric suspended particle-retaining quality of plant leaves varied in the range of 0.65˘0.23 (Pi. tabuliformis) to 3.50˘1.83 mg/cm 2 (Po. davidiana); the light shaded areas by particles on leaves were in the range of 4.26˘0.02 (Pi. tabuliformis) to 45.96˘2.42 cm 2 /leaf (Po. davidiana); and the atmospheric particle-retaining horizontal density of leaves was in the range of 97˘2 (Pi. tabuliformis) to 147˘7 per cm 2 (Po. davidiana). The broad-leaved plants, particularly Po. davidiana, R. hugonis and B. platyphylla, were appropriate species for purification of atmospheric particles. Plants with lower dust-retention abilities than the above three species (e.g., R. xanthina, Pe. sepium, S. salicifolia and V. negundo var. heterophylla) could be alternative plants for purification. However, the needle-leaved plant Pi. tabuliformis was not recommended as a tree species for purification of atmospheric particles in the core area of the Mount Wutai scenic area.

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Genotoxicity assessment of particulate matter emitted from heavy-duty diesel-powered vehicles using the in vivo Vicia faba L. micronucleus test

Cited by 28 publications

References 31 publications

Chromosomal and Nuclear Alterations in the Root Tip Cells of Vicia faba Induced by Sodium Chloride

Chromosomal and Nuclear Alterations in the Root Tip Cells of Vicia faba Induced by Sodium Chloride

The Toxicological Mechanisms of Environmental Soot (Black Carbon) and Carbon Black: Focus on Oxidative Stress and Inflammatory Pathways

Retention of Atmospheric Particles by Local Plant Leaves in the Mount Wutai Scenic Area, China

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