Genotoxicity and Immunotoxicity of Wastewater Effluents Discharged from Vilnius Wastewater Treatment Plant

Baršienė, Janina; Andreikėnaitė, Laura; Vosylienė, Milda Zita; Milukaitė, A.

doi:10.2478/v10043-009-0025-4

Cited by 6 publications

(5 citation statements)

References 41 publications

(38 reference statements)

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“…in diploid number and shows very close relative length values compared to Asymphylodora sp. 1 described by Baršienė (1993), but differs in centromere position of corresponding chromosome pairs. Such variation in centromere position of corresponding chromosomes of related species is most easily explained by pericentric inversions.…”

Section: Discussionmentioning

confidence: 96%

“…The only karyotype of Palaeorchis sp. known so far from B. tentaculata includes 14 chromosomes and does not show any similarity to the chromosome sets of Asymphylodora species (Baršienė, 1993). The chromosome set revealed for P. incognitus in this study, with 2 n = 20, clearly differs from the karyotype of its congener with 2 n = 14.…”

Section: Discussionmentioning

confidence: 98%

“…The chromosome number and morphology of only five lissorchiid species have been analysed. Diploid chromosome sets of three unidentified species of larval Asymphylodora , developing in B. tentaculata , comprise 20 or 22 elements with two or one pair of large metacentric elements, respectively (Baršienė, 1993). The karyotype with 2 n = 20 and with similar chromosome morphology was described for unidentified larval stages of lissorchiid from the snails Melanopsis dufouri (Férussac, 1823) collected in Spain (Baršienė et al ., 1995).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Exploring species diversity of lissorchiid trematodes (Digenea: Lissorchiidae) associated with the gravel snail,Lithoglyphus naticoides, in European freshwaters

2020

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Comparative analysis using complete ITS2 and partial 28S rDNA sequence data revealed that cercariaeum developing in rediae in Lithoglyphus naticoides represent two different lissorchiid species. One morphotype of cercariaeum is conspecific with adult Palaeorchis incognitus from European roach, Rutilus rutilus. The other cercariaeum is attributable to the genus Asymphylodora, but the species identity is not yet determined. We also generate the first rDNA sequences for Asymphylodora progenetica based on new collections from Bithynia tentaculata from Lithuania. Phylogenetic analyses of the newly generated sequences, together with information for other lissorchiids available on GenBank, showed that all representatives of Lissorchiidae form a strongly supported clade. Three monophyletic lineages, Asymphylodora, Palaeorchis and Lissorchis, were recognized at the generic level. Karyological analysis of the chromosome set of larval P. incognitus revealed a diploid number of 2n = 20. Its karyotype with subtelocentric chromosomes prevailing can be regarded as comparatively ‘primitive’, which is consistent with the basal position of P. incognitus in the 28S tree relative to the representatives of the genus Asymphylodora. The present study adds significant new information for establishing species-specific markers for the confident characterization of different developmental stages of lissorchiid species and clarification of their life cycles.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Exploring species diversity of lissorchiid trematodes (Digenea: Lissorchiidae) associated with the gravel snail,Lithoglyphus naticoides, in European freshwaters

2020

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“…Turbidity also limits the bactericidal effect of chlorine in the wastewater during disinfection [ 35 ] and may react with organic compounds in the water to form micro-contaminants such as trihalomethane (THM). THMs are carcinogenic and render the water unfit for recreational activities to consumers who may depend on the receiving water shed for such [ 9 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Antimicrobial Resistant and Virulent Salmonella spp. in Treated Effluent and Receiving Aquatic Milieu of Wastewater Treatment Plants in Durban, South Africa

Odjadjare

Olaniran

2015

IJERPH

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In this study, we evaluated the impact of treated wastewater effluent from two wastewater treatment plants on the physicochemical parameters and Salmonella spp. load of receiving rivers. Presumptive Salmonella spp. were obtained at all sampled points including the discharge points, with counts ranging from 0 to 4.14 log cfu/mL at both plants. Turbidity, chemical and biological oxygen demand were found to be high and mostly above the required limit for treated wastewater discharge. However, recorded nitrate and phosphate values were very low. Of the 200 confirmed Salmonella spp. isolates recovered from the treated effluent and receiving surface waters, 93% harbored the spiC gene, 84% harbored the misL gene, and 87.5% harbored the orfL gene while 87% harbored the pipD gene. The antibiotic resistance profile revealed that the isolates were resistant to sulfamethoxazole, nalidixic acid and streptomycin, but susceptible to quinolones and third generation β-lactams. These results indicate that in South Africa treated effluents are still a major source of contamination of rivers with pathogens such as Salmonella. Appropriate steps by the regulatory authorities and workers at the treatment plants are needed to enforce stipulated guidelines in order to prevent pollution of surface water resources due to the discharge of poorly treated effluents.

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“…The elevation of micronuclei increased progressively with increasing the duration of exposure. Barsiene et al [46] assessed the genotoxicity, cytotoxicity and immunotoxicity potential of treated wastewater effluents (TWE) discharged from the Vilnius Wastewater Treatment Plant. The analysis of micronuclei, nuclear buds (genotoxicity endpoints), bi-nucleated and fragmented apoptotic cells (cytotoxicity endpoints) in peripheral blood of rainbow trout (Oncorhynchus mykiss) after a 12-day exposure to TWE revealed a significant increase of micronuclei in exposed O. mykiss specimens compared to control fish.…”

Section: Micronuclei and Nuclear Lesions Testsmentioning

confidence: 99%

Genotoxicity Tests and Their Contributions in Aquatic Environmental Research

Osman¹

2014

JEP

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As many chemicals with genotoxic potential are emitted to surface water, genotoxicity tests are gaining importance which led to the development of several techniques to detect directly DNA damage. The relevance of detecting the genotoxic risks associated with water pollution was firstly perceived in the late 1970s. Since that time several tests have been developed for evaluating DNA alterations in aquatic animals. These tests rely on the premise that any changes to DNA may have long-lasting and profound consequences. Sister chromatid test, chromosome aberrations, comet assay, and micronucleus test are currently the most widely employed methods to detect DNA lesions in ecotoxicology. Chromosomal aberration and sister chromatid exchanges are time consuming, resource intensive and require proliferating cell population. Hence, Comet assay and Micronucleus test as cost effective and more sensitive test systems have now been introduced for assessing the genotoxicity of chemicals. This review presents a synthesis of the state of the art in the methodologies of comet assay and micronucleus test and their contributions in aquatic environmental research. The text explores the latest knowledge and thinking on these very important approaches for the assessment of environmental health, management, and conservation. The primary concern of the present review is the measurement of genotoxic potential in aquatic organisms under field and laboratory conditions, where effects of chemicals at different levels of biological organization can be examined.

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Genotoxicity and Immunotoxicity of Wastewater Effluents Discharged from Vilnius Wastewater Treatment Plant

Cited by 6 publications

References 41 publications

Exploring species diversity of lissorchiid trematodes (Digenea: Lissorchiidae) associated with the gravel snail,Lithoglyphus naticoides, in European freshwaters

Exploring species diversity of lissorchiid trematodes (Digenea: Lissorchiidae) associated with the gravel snail,Lithoglyphus naticoides, in European freshwaters

Prevalence of Antimicrobial Resistant and Virulent Salmonella spp. in Treated Effluent and Receiving Aquatic Milieu of Wastewater Treatment Plants in Durban, South Africa

Genotoxicity Tests and Their Contributions in Aquatic Environmental Research

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