Genotoxicity and cytotoxicity of three microcystin-LR containing cyanobacterial samples from Antioquia, Colombia

Herrera, Natalia; Herrera, Carolina; Ortiz, I.; Orozco, Luz Yaneth; Robledo, Sara M.; Agudelo, Diana; Echeverri, Fernándo

doi:10.1016/j.toxicon.2018.09.011

Cited by 18 publications

(25 citation statements)

References 46 publications

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“…MCs detection by HPLC depends on on-site sampling, sample processing and laboratory analysis which can be time demanding. Moreover, due to its low selectivity and time response, it is not suitable for rapid processing of multiple samples [ 75 , 77 , 94 , 100 , 129 ]. HPLC is also constrained by the large number of diverse MC variants and the commercial availability of standard compounds without which identification of MC becomes impossible.…”

Section: Analytical Methods To Detect Microcystinsmentioning

confidence: 99%

A Mini-Review on Detection Methods of Microcystins

Massey

Jia

et al. 2020

Toxins

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Cyanobacterial harmful algal blooms (CyanoHABs) produce microcystins (MCs) which are associated with animal and human hepatotoxicity. Over 270 variants of MC exist. MCs have been continually studied due of their toxic consequences. Monitoring water quality to assess the presence of MCs is of utmost importance although it is often difficult because CyanoHABs may generate multiple MC variants, and their low concentration in water. To effectively manage and control these toxins and prevent their health risks, sensitive, fast, and reliable methods capable of detecting MCs are required. This paper aims to review the three main analytical methods used to detect MCs ranging from biological (mouse bioassay), biochemical (protein phosphatase inhibition assay and enzyme linked immunosorbent assay), and chemical (high performance liquid chromatography, liquid chromatography-mass spectrometry, high performance capillary electrophoresis, and gas chromatography), as well as the newly emerging biosensor methods. In addition, the current state of these methods regarding their novel development and usage, as well as merits and limitations are presented. Finally, this paper also provides recommendations and future research directions towards method application and improvement.

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Section: Analytical Methods To Detect Microcystinsmentioning

confidence: 99%

A Mini-Review on Detection Methods of Microcystins

Massey

Jia

et al. 2020

Toxins

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“…To ensure a greater infection of macrophages in vitro, the L. (V) panamensis strain was maintained by successive passages in experimentally infected hamsters (Mesocricetus auratus), making periodic aspirations of hamster lesions using PBS and #26 needles. The aspirated samples were grown in NNN culture medium at 26 o C, until promastigotes were obtained, which were used to infect U937 macrophages as described below [54][55][56][57] .…”

Section: Biological Activity Assaysmentioning

confidence: 99%

“…After this incubation period, 100 μL/well of a solution of 50% isopropanol (Merck Millipore) and 10% sodium dodecyl sulfate (SDS) (Merck Millipore) was added, in order to solubilize the formazan crystals formed. The plates were incubated for another 30 minutes and the production of formazan (which is proportional to the percentage of viable cells) was measured in a microplate reader (Benchmark Bio-Rad Hercules, CA, USA) at a wavelength of 570 nm 54 .…”

Section: Biological Activity Assaysmentioning

confidence: 99%

“…The tests were carried out twice, with three replicates for each concentration evaluated. The cytotoxicity of each compound was classi ed, according to the LC 50 values, using the following scale: high cytotoxicity, LC 50 < 100 µg/mL; moderate cytotoxicity, 100 < LC 50 < 200 μg/mL; and low cytotoxicity, LC 50 > 200 μg/mL 54 .…”

Section: Biological Activity Assaysmentioning

confidence: 99%

“…Parasite. The L. (V) panamensis strain M / HOM / 87 / UA140 was used, transfected with the gene for green uorescent protein (UA140-EGFP) generated in a previous work 54 . Parasites were cultured as promastigotes in Novy-MacNeil-Nicolle (NNN) biphasic medium and phosphate buffered saline (PBS) with glucose (pH 6.9) as the liquid phase.…”

Section: Biological Activity Assaysmentioning

confidence: 99%

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Theoretical and Experimental Study of New Dihydroorotate Dehydrogenase and Tryparedoxin Peroxidase Inhibitors: One More Step in the Study of Leishmaniasis Infection

Espinosa-Saez

Robledo

Pineda

et al. 2021

Preprint

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In this study, the viability of new dihydroorotate dehydrogenase and tryparedoxin peroxidase inhibitors is reported. In vitro antileishmanial activity was evaluated using a Leishmania (V) panamensis strain, and the cytotoxicity of the compounds was assessed using U-937 cells. The in vivo therapeutic response was evaluated in golden hamsters (Mesocricetus auratus) experimentally infected with L. (V) panamensis and treated with a 1% topical formulation of compounds 4a–f. On the other hand, in silico studies considering the synthesized compounds were also carried out. All of the compounds showed promising in vitro activity, with mean EC50 effective concentration values ranging from 3.8 µM to 19.3 µM. Likewise, treatment with compounds 4a–f produced improvement in most of the hamsters and cured some; in particular, those treated with compounds 4b, 4c, 4d, and 4f reacted the best. Molecular dynamics (MD) simulations, computational docking, and MM/GBSA studies indicate the promising bioavailability and absorption characteristics of the studied compounds, which are expected to be orally active. In addition, the studied 2-arylquinolines are absorbable at the blood–brain barrier, but not in the gastrointestinal tract. Finally, ADMET properties suggest that these molecules can be safely used as leishmaniasis inhibitors.

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