Genotoxic responses of juvenile tilapia (Oreochromis niloticus) exposed to florfenicol and oxytetracycline

Botelho, Rafael Guimarães; Christofoletti, Cintya Aparecida; Correia, Jorge Evangelista; Ansoar, Yadira; Olinda, Ricardo Alves de; Tornisielo, V.L.

doi:10.1016/j.chemosphere.2015.02.053

Cited by 83 publications

(32 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The radicals can directly react with biomolecules, such as DNA, or may indirectly act as signalling molecules to mediate DNA damage (Wang et al, ). In this study, the MDA and PC contents showed a significant increase, and the F value decreased with the injection of 40 mg/kg FLR; these results suggest a certain degree of genotoxicity and damage caused by lipid peroxidation and are consistent with those of previous studies (Botelho et al, ; Dorts et al, ). In this study, higher levels of PC and MDA and lower F values were found in the SIs.…”

Section: Discussionsupporting

confidence: 93%

Comparison of the florfenicol metabolic rate in Portunus trituberculatus individuals with different body weights

et al. 2018

View full text Add to dashboard Cite

We analysed the metabolic rate of large individuals (LIs) and small individuals (SIs) (based on body weight) of 80-day-old specimens of Portunus trituberculatus injected with 40 mg/kg florfenicol (FLR). The results showed that FLR contents in the gills and hepatopancreas were higher in the SIs than in the LIs. The activities of erythromycin N-demethylase and 7-ethoxyresorufin-O-deethylase related to cytochrome oxidase P450 (CYP) were induced after FLR injection and showed a sizedependent pattern; however, no significant difference in aminopyrine N-demethylase activity was found between the LIs and SIs. The activity of the phase II enzyme glutathione S-transferase (GST) increased in the hepatopancreas after FLR injection, and it was higher in the SIs than the LIs. Moreover, induction of CYP2, CYP3, GST, ABCB and ABCG was observed in the first 24 hr after FLR injection.FLR caused more biomolecular damage in the SIs than the LIs, displaying an obvious body weight-and time-dependent pattern. In summary, the SIs exhibited faster FLR uptake and slower depuration in this species, and FLR caused more biomolecular damage to the SIs. K E Y W O R D Saccumulation, biomolecular damage, body weight, detoxification, florfenicol, Portunus trituberculatus

show abstract

Section: Discussionsupporting

confidence: 93%

Comparison of the florfenicol metabolic rate in Portunus trituberculatus individuals with different body weights

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Micronucleus assay has been used as a measure to assess genotoxicity in organisms caused by different pesticides under laboratory as well as field condition (Lajmanovich et al, 2005; Yin et al, 2009; Botelho et al, 2015). The formation of micronuclei was observed in pesticide affected groups of the silkworm and the rate of micro nuclei formation was also increased with the increased sub lethal doses of chlorpyrifos.…”

Section: Discussionmentioning

confidence: 99%

Larval Exposure to Chlorpyrifos Affects Nutritional Physiology and Induces Genotoxicity in Silkworm Philosamia ricini (Lepidoptera: Saturniidae)

2016

View full text Add to dashboard Cite

Chlorpyrifos is a most widely used organophosphate insecticide because of its cost effectiveness and degradable nature. However, this pesticide enters and contaminates the environment either by direct application, spray drifts or crop run off and shows adverse effect on the non-targeted organisms. Philosamia ricini (eri silkworm), one of the most exploited, domesticated and commercialized non mulberry silkworm is known for mass production of eri silk. The silkworm larvae get exposed to pesticide residues on the leaves of food plants. The present study investigates the effect of commercial formulation of chlorpyrifos (Pyrifos-20 EC) on eri silkworm. Initially the LC50 value of chlorpyrifos was determined at 24–96 h and further experiments were carried out with sub lethal concentrations of the chlorpyrifos after 24 h of exposure period. The potential toxicity of chlorpyrifos was evaluated as a fuction of metabolism and nutritional physiology in 3rd, 4th, and 5th instar larvae. Alteration in histoarchitecture of 5th instar eri silkworm gut exposed to sub lethal concentration of chlorpyrifos formulation was also studied. Chlorpyrifos induced genotoxicity in silkworm hemocytes was also investigated by single cell gel electrophoresis, micronuclei assay, and apoptosis assay. Herein, LC50 values of chlorpyrifos were calculated as 3.83, 3.35, 2.68, and 2.35 mg/L at 24, 48, 72, and 96h respectively. A significant decrease in trehalose activity along with digestive enzyme activity was observed in chlorpyrifos affected groups (P < 0.05). Further, genotoxicity study revealed higher tail percentage, tail length and tail moment of the damage DNA in chlorpyrifos exposed groups (P < 0.001). Moreover, at 2.0 mg/L concentration, ~10 fold increases in tail length was observed as compared to the control. Results showed activation of caspase activity following 24 h chlorpyrifos exposure (1.5 and 2.0 mg/L) in a dose-dependent manner. Moreover, in control group less number of apoptotic cells was detected, however in both chlorpyrifos exposed groups' numbers of apoptotic cells were statistically higher (P < 0.001). Taken together, this study provides evidence that chlorpyrifos pollution might have adverse effect on overall nutritional physiology and genotoxicity of eri silkworm that could lead to reduced survivability of this economically beneficial insect.

show abstract

“…The bioassays were set up in aquariums (40 L) (size: length D 45 cm, height D 25 cm, and width D 20 cm). The fish were exposed to 96 h of treatment, according to other studies with fish, [19][20][21] with constant aeration at a temperature of 23 §2 C, pH D 8.3, and photoperiod of 14-h light/10-h darkness. Eight aquariums were used in the experiment; i.e.…”

Section: Experimental Designmentioning

confidence: 99%

Liver alterations inOreochromis niloticus(Pisces) induced by insecticide imidacloprid: Histopathology and heat shock proteinin situlocalization

Ansoar-Rodríguez

Christofoletti

Correia

et al. 2016

Journal of Environmental Science and Health, Part B

View full text Add to dashboard Cite

Liver is very sensitive to environmental contaminants such as pesticides, it being the first target of toxicity of a substance. The objective of this study was to investigate the possible effects of the insecticide imidacloprid (IMI) on the liver of Oreochromis niloticus according concentrations used for growing sugarcane. A semi-quantitative analysis of histopathological alterations of IMI on liver was performed by light microscopy and cellular labeling of heat shock proteins (HSP70) by immunohistochemistry. The most common changes in liver at all concentrations of IMI were hydropic degeneration, pyknotic nuclei, and loss of cell limits. Steatosis and increased levels of HSP70 were detected in hepatocytes with the highest concentration of IMI. In conclusion, the tested concentrations of IMI induced histopathological changes in the liver of O. niloticus and active defence mechanisms to maintain the morphophysiological integrity of the liver. This insecticide has a toxicity potential for these fish, which is a non-target organism of its action.

show abstract

Genotoxic responses of juvenile tilapia (Oreochromis niloticus) exposed to florfenicol and oxytetracycline

Cited by 83 publications

References 45 publications

Comparison of the florfenicol metabolic rate in Portunus trituberculatus individuals with different body weights

Comparison of the florfenicol metabolic rate in Portunus trituberculatus individuals with different body weights

Larval Exposure to Chlorpyrifos Affects Nutritional Physiology and Induces Genotoxicity in Silkworm Philosamia ricini (Lepidoptera: Saturniidae)

Liver alterations inOreochromis niloticus(Pisces) induced by insecticide imidacloprid: Histopathology and heat shock proteinin situlocalization

Contact Info

Product

Resources

About