Genotoxic, metabolic, and biological responses of Chironomus sancticaroli Strixino &amp; Strixino, 1981 (Diptera: Chironomidae) after exposure to BBP

Morais, Gisele dos Santos; Vieira, Thalita Bastida; Santos, Gustavo Souza; Dolatto, Rafael Garrett; Cestari, Marta Margarete; Grassi, Marco Tadeu; Navarro-Silva, Mário Antônio

doi:10.1016/j.scitotenv.2020.136937

Cited by 13 publications

(3 citation statements)

References 52 publications

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“…The obtained results are in line with previous studies in other organisms, which have confirmed that BBP can induce different types of damage such as apoptosis 48 , genotoxicity 49 , oxidative stress 50 , stress response activation 45 , or endocrine disruption 14 . Although there are studies in invertebrates showing the impact on development and other physiological processes 39 , 51 , most of them did not focus on the putative mode of action, with only a few of them trying to delve into the response mechanisms.…”

Section: Discussionsupporting

confidence: 91%

Analysis of the impact of three phthalates on the freshwater gastropod Physella acuta at the transcriptional level

Prieto-Amador

Caballero

Martínez-Guitarte

2021

Sci Rep

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Plastic pollution is one of the leading environmental problems. Phthalates are widely used plastic additives released into the environment. Although the effects of phthalates on vertebrates have been extensively studied, there is a knowledge gap regarding their effects on invertebrates. This work analyzes the impact of three phthalates, diethyl phthalate (DEP), benzyl butyl phthalate (BBP), and bis-(2-ethylhexyl) phthalate (DEHP), on the gastropod Physella acuta at the molecular level to establish the putative pathways involved in its response to them. By real-time PCR, we obtained the expression profile of 30 genes in animals exposed for 1 week to 0.1, 10, and 1000 μg/L of each phthalate. The genes cover DNA repair, detoxification, apoptosis, oxidative and stress responses, immunity, energy reserves, and lipid transport. The results show that while DEP and DEHP did not alter the mRNA levels, BBP modulated almost all the analyzed genes. It can be concluded that the impact of BBP is extensive at the molecular level. However, it cannot be dismissed that the increase in transcriptional activity is a general response due to this compound’s well-known role as an endocrine disruptor. Additional research is needed to elucidate the differences observed in the impact of these compounds on the gastropod P. acuta.

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Section: Discussionsupporting

confidence: 91%

Analysis of the impact of three phthalates on the freshwater gastropod Physella acuta at the transcriptional level

Prieto-Amador

Caballero

Martínez-Guitarte

2021

Sci Rep

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“…Previous studies in other organisms have con rmed that BBP can induce different types of damage such as apoptosis 41 , genotoxicity 42 , oxidative stress 43 , stress response activation 44 , or endocrine disruption 13 . Although there are studies in invertebrates showing the impact on development and other physiological processes 38,45 , most of them did not focus on the putative mode of action, with only a few of them trying to delve into the mechanisms of response.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Impact of Three Phthalates on the Freshwater Gastropod Physella Acuta at the Transcriptional Level

Prieto-Amador

Caballero

Martínez-Guitarte

2021

Preprint

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Plastic pollution is one of the leading environmental problems. Phthalates are widely used plastic additives released into the environment. Although the phthalates' effects have been extensively studied on vertebrates, there is a gap in knowledge on their effects on invertebrates. This work analyzes the impact of three phthalates, diethyl phthalate (DEP), benzyl butyl phthalate (BBP), and bis-(2-ethylhexyl) phthalate (DEHP), on the gastropod Physella acuta at the molecular level to establish the putative pathways involved in its response to them. By real-time PCR, we obtained the expression profile of thirty genes in one-week exposed animals at 0.1, 10, and 1000 µg/L. The genes cover the DNA repairing mechanism, detoxification mechanisms, apoptosis, oxidative and stress responses, immunity, energy reserves, and lipid transport. The results show that while DEP and DEHP did not cause alteration of the mRNA levels, BBP modulates almost all the genes tested. It can be concluded that the impact of BBP is extensive at the molecular level. However, it cannot be dismissed that the increase in transcriptional activity is a general response due to this compound’s well-known role as an endocrine disruptor. Additional research is needed to elucidate the differences observed in the impact of these compounds on this gastropod.

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“…It has been reported that BBP can promote breast cancer stem cell expansion (Cao et al, 2023), participate in DNA damage and methylation (Tsai et al, 2014), and induce significant neurodevelopmental damage (Miodovnik et al, 2014). In addition to humans, this pollutant has been shown to have toxic effects on other organisms, including chironomids (Call et al, 2001;dos Santos Morais et al, 2020;Llorente Ortega, 2022;Planello et al, 2011), cladocerans (Li et al, 2021;Wang et al, 2011), mice and rats (Ambe et al, 2019;Agas et al, 2007;Ema et al, 2003;Ema & Miyawaki, 2002;Gray et Chemia Naissensis, Vol 6, Issue 1, RESEARCH ARTICLE, 22-40 DOI: 10.46793/ChemN6.1.22P 25 al., 2000;Piersma et al, 2000), the marine univalve Haliotis diversicolor super-texta (Liu et al, 2009), daphnia (Gledhill et al, 1980), fish (Bhaisare et al, 2022;Gledhill et al, 1980) and algae (Gledhill, 1980). The structure and physiochemical properties of BBP are shown in Table 1.…”

Section: Introductionmentioning

confidence: 99%

The influence of benzyl butyl phthalate on the growth of several phytoplankton species (Microcystis sp., Anabaena variabilis, Chlorella sp., Scenedesmus sp.) in laboratory conditions

Petronijević,

Milošević,

Kostić Kokić

et al. 2023

ChemN

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Phthalic acid esters (PAEs) are organic compounds extensively used as plasticisers. Their widespread use has resulted in their presence in aquatic and terrestrial ecosystems, making them a high-risk pollutant. PAEs are detrimental to human health as they disrupt the endocrine system and can potentially cause cancer. Although their impact on humans is relatively well-known, more research is necessary to comprehend their effects on phytoplankton. This work aimed to examine the influence of different concentrations (50, 100, 150, 200, 250 µg/L) of benzyl butyl phthalate (BBP) on the growth of several most common phytoplankton species (Microcystis sp., Anabaena variabilis, Chlorella sp., Scenedesmus sp.) in laboratory conditions. Phytoplankton growth was monitored spectrophotometrically to determine the concentration of chlorophyll a. The results showed that higher concentrations of BBP significantly inhibited the growth of A. variabilis and Microcystis sp. Green algae showed a considerably lower sensitivity, especially Chlorella sp., where significant growth inhibition was not observed. After the experiment, the detection and quantification of BBP in extract samples were performed using gas chromatography with mass spectrometry (GC-MS). BBP was detected only in the extracted sample with Scenedesmus sp., but the detected concentration was insignificant. The results indicate that all tested organisms could probably absorb and metabolize BBP, of which Scenedesmus sp. has the least ability.

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Genotoxic, metabolic, and biological responses of Chironomus sancticaroli Strixino & Strixino, 1981 (Diptera: Chironomidae) after exposure to BBP

Cited by 13 publications

References 52 publications

Analysis of the impact of three phthalates on the freshwater gastropod Physella acuta at the transcriptional level

Analysis of the impact of three phthalates on the freshwater gastropod Physella acuta at the transcriptional level

Analysis of the Impact of Three Phthalates on the Freshwater Gastropod Physella Acuta at the Transcriptional Level

The influence of benzyl butyl phthalate on the growth of several phytoplankton species (Microcystis sp., Anabaena variabilis, Chlorella sp., Scenedesmus sp.) in laboratory conditions

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