Genotoxic evaluation of different doses of inorganic lead (PbII) in Hoplias malabaricus

Abstract

Different genetic biomarkers have been used to evaluate the pollution effects of mutagenic agents such as metals and also a great variety of chemicals delivered on the environment by human activities. This way, the aim of the present report was to evaluate the effects of inorganic lead in fishes through the frequency of piscine micronuclei and nuclear morphological alterations in peripheral cells, chromosomal aberration frequency and comet assays in blood and kidney cells. Specimens of Hoplias malabaricus received different doses of lead by intra-peritoneal injections at time of 96 h. There was not a significant difference between control and treated groups for the piscine micronucleus and chromosomal aberration assays. In the comet assays there was a significant difference between control and contaminated groups. However, a significant difference between the applied doses was not observed. The results obtained with the comet assays also show that blood presented a higher sensibility than the kidney tissue, possibly due to the acute contamination. Although the results showed the genotoxic potential of lead at the 21 and 63 microg Pb(2 + )/g doses for both tissues, the lowest dose is considered more appropriate for future bioassays.

Studies of micronuclei and other nuclear abnormalities in red blood cells of Colossoma macropomum exposed to methylmercury

“…Thus, when considering frequency per 1,000 cells, MNs were 0.08 ± 0.13 in Hoplias malabaricus (Ferraro et al , 2004), 0.1 ± 0.316 in Eigenmannia virescens (Bücker et al , 2006), 3.17 ± 0.48 in Carassius auratus (Çavas and Könen, 2007), and 2.4 ± 1.19 in Colossoma macropomum (present paper). Furthermore, in MN assaying undertaken by Ramsdorf et al (2009), using H. malabaricus, there were no MNs, only nuclear morphological alterations.…”

Assessing genotoxic effects in fish from a marine protected area influenced by former mining activities and other stressors

“…Pb in muscle was also frequently linked to genotoxicity in the current study. Previous studies reported genotoxic effects on Hoplias malabaricus (Ramsdorf et al, 2009) and Prochilodus lineatus (Monteiro et al, 2011) in response to inorganic Pb (Pb II) exposure. This metal has also been associated with lethality in fish, as well as with decreased reproductive performance, growth inhibition, and changes in behavior under sub-lethal exposure (Burdena et al, 1998).…”

“…Variations in the damage to genetic material is expected in different tissues because of the differential background levels of DNA single-strand breaks in tissues with different amounts of excision repair, and also because of different metabolism levels and concentrations of antioxidants (Lee and Steinert, 2003;Ali et al, 2008). The higher responsiveness of blood tissue to contaminants was explained due to the greater lifespan of circulating erythrocytes in comparison to cells in kidney, liver, and gill tissues (Ramsdorf et al, 2009).…”

“…DNA damage was observed using a Leica® epifluorescence microscope at a magnification of 400×. For each fish, 100 nucleoids were visually analyzed according to the method described by Ramsdorf et al (2009).…”

Effects of water-soluble fraction of petroleum on growth and prey consumption of juvenile Hoplias aff. malabaricus (Osteichthyes: Erythrinidae)

“…In the natural environment, the trahira H. aff. malabaricus exhibits ambush feeding on fish species, thereby expending low levels of energy on predation (Ferreira, 2007;Ramsdorf et al, 2009;Petry et al, 2010). The small artificial environment in which this experiment was carried out favored prey detection by the juveniles, which did not spend much energy on feeding activity.…”