Genotoxic effects of Roundup® on the fish Prochilodus lineatus

Cavalcante, Dalita Gomes Silva Morais; Martinez, Cláudia Bueno dos Reis; Sofia, Silvia Helena

doi:10.1016/j.mrgentox.2008.06.010

Cited by 226 publications

(115 citation statements)

References 52 publications

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“…Immediately after being removed from the aquaria, fish were anaesthetized in benzocaine 5%, for a maximum of 2 min, for blood collection by caudal vein (Cavalcante et al, 2008). Blood samples were stored on ice for the comet assay.…”

Section: Methodsmentioning

confidence: 99%

“…The comet assay was performed using the alkaline (pH > 13) version developed by Singh et al (1988), with the modifications detailed by Cavalcante et al (2008), using two cell types: erythrocytes and gill cells. For gills dissociation firstly the organ was cleaned with phosphate buffered saline (PBS: 126.6 mM NaCl, 4.8 mM KCL, 1.5 mM CaCl, 3.7 mM NaHCO 3 , 8.9 mM Na 2 HPO 4 , 2.9 mM NaH 2 PO 4 ), gill filaments were sectioned and the pieces were transferred to microtubes, incubated for 15 min at 30°C in 0.05% trypsin (diluted in PBS Ca +2 and Mg +2 free) and homogenized by periodic manual inversion at room temperature.…”

Section: Analysis Of Dna Damage -Comet Assaymentioning

confidence: 99%

See 1 more Smart Citation

Biochemical and genetic alterations in the freshwater neotropical fish Prochilodus lineatus after acute exposure to Microcystis aeruginosa

Garcia

Martinez

2012

Neotrop. ichthyol.

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Microcystins are secondary metabolites produced by different species of cyanobacteria, such as Microcystis aeruginosa (MA). In this study, the biochemical and genetic effects of lyophilized MA were evaluated in the neotropical fish Prochilodus lineatus exposed to 1 or 2 mg L -1 lyophilized MA (treated group) or only water (control group) in static toxicity tests for 24 and 96 h. The gills and liver were used in the analysis of biotransformation enzymes and antioxidant defenses, blood and gill cells in genetic analysis and in brain and muscle it was determined the activity of acetylcholinesterase (AChE). The results showed the biotransformation pathway activation due to the increase in hepatic CYP1A and in branchial and hepatic glutathione Stransferase (GST). The antioxidant defense proved to be greatly affected by MA exposure leading to changes, both in gills and liver, in the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and in the content of tripeptide glutathione (GSH). Lipid peroxidation was not detected, but damage to DNA molecule was observed in blood cells. In conclusion, it can be state the lyophilized MA is able to promote changes in the biochemical and genetic parameters of P. lineatus.As microcistinas são metabólitos secundários produzidos por diferentes espécies de cianobactérias, como a Microcystis aeruginosa (MA). Neste estudo, os efeitos bioquímicos e genéticos de liofilizado de MA foram avaliados para juvenis da espécie de peixe Neotropical Prochilodus lineatus expostos a 1 ou 2 mg L -1 de liofilizado de MA (grupo tratado) ou apenas à água (grupo controle), em testes de toxicidade estáticos, durante 24 e 96 h. As brânquias e o fígado foram usados para as análises das enzimas de biotransformação e defesas antioxidantes, células do sangue e das brânquias para análises genéticas e no cérebro e músculo foi determinada a atividade da acetilcolinesterase (AChE). Os resultados mostraram ativação da via de biotransformação devido ao aumento da atividade da CYP1A hepática e da atividade da glutationa S-transferase (GST) hepática e branquial. As defesas antioxidantes foram muito afetadas pela exposição a MA levando a alterações, tanto no fígado como nas brânquias, na atividade da superóxido dismutase (SOD), da catalase (CAT), da glutationa peroxidase (GPx), glutationa redutase (GR) e no conteúdo do tripeptídeo glutationa (GSH). Apesar dessas alterações a peroxidação lipídica não foi detectada em nenhum dos tecidos, mas danos na molécula de DNA foram observados nas células do sangue. Em conclusão, pode-se afirmar que o liofilizado de MA é capaz de promover alterações em parâmetros bioquímicos e genéticos de P. lineatus.

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Section: Methodsmentioning

confidence: 99%

Section: Analysis Of Dna Damage -Comet Assaymentioning

confidence: 99%

Biochemical and genetic alterations in the freshwater neotropical fish Prochilodus lineatus after acute exposure to Microcystis aeruginosa

Garcia

Martinez

2012

Neotrop. ichthyol.

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“…Cytotoxicity by oxidative stress by Roundup R has been shown by transcriptomic profiling in carp and zebrafish (Uren Webster and Santos, 2015;Sulukan et al, 2017). Acute toxicity, harmful physiological effects including hepatotoxicity, neurotoxicity, deteriorated sperm counts, early life stage development and DNA-damaging effects have been reported for numerous other fish species as well for sublethal exposures to Roundup R products including Roundup Original R , Roundup Transorb R and Roundup WG R , Garlon R , and other glyphosate-based herbicides (Soso et al, 2007;Cavalcante et al, 2008;Guilherme et al, 2010Guilherme et al, , 2014aModesto and Martinez, 2010;Shiogiri et al, 2012;Ghisi and Cestari, 2013;Nwani et al, 2013;Marques et al, 2014;Moreno et al, 2014;Navarro and Martinez, 2014;Richard et al, 2014;Sinhorin et al, 2014;Braz-Mota et al, 2015;Menéndez-Helman et al, 2015;Li et al, 2016;de Moura et al, 2017;Sánchez et al, 2017;Gonçalves et al, 2018;Zebral et al, 2018) or POEA (Yusof et al, 2014). Roundup R was found to disrupt 17β-estradiol and reduce glutathione concentration in the liver of the endangered fish species delta smelt (Hypomesus transpacificus) upon 6 h of exposure at levels corresponding to 78 µg/l glyphosate concentrations and above (Jin et al, 2018).…”

Section: Registration Of Glyphosate In the European Unionmentioning

confidence: 99%

Re-registration Challenges of Glyphosate in the European Union

Székacs

Darvas

2018

Front. Environ. Sci.

104

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“…Fish are important indicators of water pollution and the emergence/rate of erythrocytes nuclear morphological abnormalities (ENMAs), including the erythrocytes micronuclei (MN), are being used to examine both the exposure and effect of mutagenic compounds. Indeed, the formation of MN in fish has been increasingly used, in field and laboratorial studies, to detect both the genotoxic potential of pollutants and as an indicator of chromosomal injury (Al-Sabti and Hardig 1990;Al-Sabti and Metcalfe 1995;Baršienė et al 2012;Bolognesi et al 2006;Buschini et al 2004;Cavalcante et al 2008;De Flora et al 1993;Hooftman and de Raat 1982;Hose et al 1987;Minissi et al 1996;Rocha et al 2009;Vigano et al 2002;Winter et al 2007). Besides, if by the one hand the appearance of MN in fish is valuable in ecological risk assessment-even able to disclose the risk of human exposure to drinking water with genotoxicants-on the other hand, the affected fish also pose risks, as feeding is well established as a major toxicant exposure route in human populations (Oates and Cohen 2009;Tijhuis et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Frequency of micronuclei and of other nuclear abnormalities in erythrocytes of the grey mullet from the Mondego, Douro and Ave estuaries—Portugal

Carrola

Santos

Rocha

et al. 2014

Environ Sci Pollut Res

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Fish are bioindicators of water pollution, and an increased rate of their erythrocyte nuclear morphological abnormalities (ENMAs)-and particularly of erythrocyte micronuclei (EMN)-is used as a genotoxicity biomarker. Despite the potential value of ENMAs and MN, there is scarce information about fish captured in Iberian estuaries. This is the case of the Portuguese estuaries of the Mondego, Douro and Ave, suffering from different levels of environmental stress and where chemical surveys have been disclosing significant amounts of certain pollutants. So, the aim of this study was to evaluate genotoxicants impacts and infer about the exposure at those ecosystems, using the grey mullet (Mugil cephalus) as bioindicator and considering the type and frequency of nuclear abnormalities of erythrocytes as proxies of genotoxicity. Sampling of mullets was done throughout the year in the important Mondego, Douro and Ave River estuaries (centre and north-western Portugal). The fish (total n=242) were caught in campaigns made in spring-summer and autumnwinter, using nets or fishing rods. The sampled mullets were comparable between locations in terms of the basic biometric parameters. Blood smears were stained with Diff-Quik to assess the frequencies of six types of ENMAs and MN (given per 1,000 erythrocytes). Some basic water physicochemical parameters were recorded to search for fluctuations matching the ENMAs. Overall, the most frequent nucleus abnormality was the polymorphic type, sequentially followed by the blebbed/ lobed/notched, segmented, kidney shaped, vacuolated, MN and binucleated. The total average frequency of the ENMAs ranged from 73‰ in the Mondego to 108‰ in the Ave. The polymorphic type was typically ≥50 % of the total ENMAs, averaging about 51‰, when considering all three estuaries. The most serious lesion-the MN-in fish from Mondego and Douro had a similar frequency (≈0.38‰), which was significantly lower than that in the Ave (0.75‰). No significant seasonal differences existed as to the MN rates and seasonal differences existed almost only in the Douro, with the higher values in AW. In general, the pattern of ENMAs frequencies was unrelated with the water physicochemical parameters. Considering the data for both the total ENMAs and for each specific abnormality, and bearing in mind that values of MN in fish erythrocytes >0.3‰ usually reflect pollution by genotoxicants, it is suggested that mullets were likely being chronically exposed to such compounds, even in the allegedly less polluted ecosystem (Mondego). Moreover, data supported the following pollution

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Genotoxic effects of Roundup® on the fish Prochilodus lineatus

Cited by 226 publications

References 52 publications

Biochemical and genetic alterations in the freshwater neotropical fish Prochilodus lineatus after acute exposure to Microcystis aeruginosa

Biochemical and genetic alterations in the freshwater neotropical fish Prochilodus lineatus after acute exposure to Microcystis aeruginosa

Re-registration Challenges of Glyphosate in the European Union

Frequency of micronuclei and of other nuclear abnormalities in erythrocytes of the grey mullet from the Mondego, Douro and Ave estuaries—Portugal

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