Genotoxic effects of anaesthetics in operating theatre personnel evaluated by the comet assay and micronucleus test

Exposure of thermoelectric power-plant workers to volatile organic compounds from fuel oil: Genotoxic and cytotoxic effects in buccal epithelial cells

“…For instance, Ç elik et al [32] and Burgaz et al [36] found a significant increase in the frequency of MNC in smokers compared with non-smokers, whereas Bolognesi et al [37] and Martino-Roth et al [38] reported a small decrease in the frequency of MNC in smokers, although this was not significant. Similar to our observations, several authors also found that the occurrence of MNC was not significantly associated with smoking status in individuals occupationally exposed to fuel derivatives [21,22,31,33] or other potentially carcinogenic substances, such as pesticides [23,24]. Regarding ONA, and fully in line with our observations, Martins et al [22] found that tobacco smoking did not significantly interfere with the cytotoxicity induced by petroleum derivates in buccal mucosa cells or in the lateral border epithelium of the tongue in petrol-station attendants.…”

“…The micronucleus assay with exfoliated cells is emerging as a preferential method to measure this type of damage [11]; it is considered a sensitive and non-invasive method for monitoring DNA damage in human populations [15,20]. Recently, such approaches have been frequently used in bio-monitoring studies of occupational exposure to carcinogenic substances [e.g., [21][22][23][24][25], but to our knowledge no study has been done for thermoelectric power-plant workers exposed to VOCs derived from fuel oil. Thus, the main objective of this study is to assess the association between such occupational exposure to VOCs and the frequency of genotoxic and cytotoxic events in the human buccal epithelium.…”

Chromosomal damage among medical staff occupationally exposed to volatile anesthetics, antineoplastic drugs, and formaldehyde

“…Cellular DNA damage increased after exposure to both desflurane and halothane (12). In the recent study of Rozgaj et al (14), increased levels of DNA damage were reported among anesthesiologists exposed to the anesthetic gases sevoflurane, isoflurane, and nitrous oxide. The authors also recorded a three-fold increase in the frequency of micronuclei, representing chromosomal damage (14).…”

“…In the recent study of Rozgaj et al (14), increased levels of DNA damage were reported among anesthesiologists exposed to the anesthetic gases sevoflurane, isoflurane, and nitrous oxide. The authors also recorded a three-fold increase in the frequency of micronuclei, representing chromosomal damage (14). These findings are in accordance with the earlier data of Lewinska et al (15), who demonstrated a significant increase in micronucleus frequency in PBL among nurses exposed to nitrous oxide.…”

Genotoxic effects of anesthetics in operating room personnel evaluated by micronucleus test

“…Neurotoxic [32], hepatotoxic [22], nephrotoxic [24,32] and carcinogenic effects [13,32,39], as well as fertility alterations, increased incidence of spontaneous abortions and congenital abnormalities have been observed in these professionals [3,24,41,45,46]. Nevertheless, there is still some controversy regarding the genotoxic and/or mutagenic effects of acute or chronic exposure to anesthetic gases [14,25,27], even though significant increases in the frequency of chromosome aberrations and of micronuclei (MN) in peripheral blood lymphocytes of professionals exposed to such agents have been reported [8,11,12,29,31,35,42,44].…”