Genotoxic effect of copper on salivary gland polytene chromosomes of Chironomus riparius Meigen 1804 (Diptera, Chironomidae)

Michailova, Paraskeva; Petrova, N. A.; Ilkova, Julia; Bovero, Stefano; Brunetti, S.; White, Kenneth; Sella, Gabriella

doi:10.1016/j.envpol.2005.12.041

Cited by 34 publications

(19 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The Al treatment was given with a concentration of 500 µg ml −1 because this concentration was found in the sediments of the long-term polluted Afon Gooch River station. The Cu treatment was given with concentrations of 0.005 µg ml −1 , 0.01 µg ml −1 and 0.05 µg ml −1 on the basis of information on Cu concentrations in rivers in the Piedmont Region, Italy (Michailova et al 2006). Treated larvae as well as control larvae were reared on sterilized sand sediment.…”

Section: Genotoxic Effects Of Experimental Exposure To Specific Tracementioning

confidence: 99%

“…In this way the supposed genotoxic effects of specific trace metals observed in the field can be measured (Michailova & Belcheva 1990;Michailova et al 2001a,b;Michailova et al 2003Michailova et al , 2006. So far, four trace metals were tested in laboratory experiments: Al, Pb, Cr and Cu (Michailova et al 2001a(Michailova et al ,b, 2003(Michailova et al , 2006. Al, Pb and Cr free ions were tested separately with a chronic exposure, starting from fertilized egg masses up to the last larval stage.…”

Section: Genotoxic Effects Of Experimental Exposure To Specific Tracementioning

confidence: 99%

“…Al, Pb and Cr free ions were tested separately with a chronic exposure, starting from fertilized egg masses up to the last larval stage. Cu ions were tested by exposing newly fertilized egg masses to different ion concentrations for 48 hours (Michailova et al 2006). Chronic Cr and Pb treatments were given with three different concentrations, one of them similar to the concentration in the sediments of two polluted Italian stations (152 µg ml −1 in Santena & Milano, see Figure 3).…”

Section: Genotoxic Effects Of Experimental Exposure To Specific Tracementioning

confidence: 99%

“…These were the station sediments that were considered unpolluted. The chemical analyses of sediments were performed by either of two methods-atomic absorption spectrophotometry with background correction by deuterium lamp, and inductively coupled plasma optical emission spectroscopy (ICPOES) (Michailova et al 1996;Michailova et al 2001b). …”

Section: Genotoxic Effects Of Trace-metal-polluted Sediments In Naturmentioning

confidence: 99%

See 3 more Smart Citations

Chironomids (Diptera) and their salivary gland chromosomes as indicators of trace-metal genotoxicity

Michailova

Sella

Petrova

2012

Italian Journal of Zoology

Self Cite

View full text Add to dashboard Cite

The long-term impact of pollution in freshwater aquatic environments can be assessed realistically only if the mutagenic effects of pollutants on organisms inhabiting aquatic biotopes are evaluated. To do this, the larvae of Chironomids (Diptera) can serve as suitable indicators of potential environmental genotoxicity. These larvae exhibit aberrations in their polytene chromosomes, which, when analyzed, indicate an early response to toxicity more efficiently than morphological analysis, up to now the usual technique. Chironomids have excellent salivary gland chromosomes with well-defined bands and permanent somatic pairing, where there are two important regions of active transcription-Balbiani rings (BRs) and Nucleolar organizers (NOR). We have described the responses of different Chironomid species in two situations: in the field and in the laboratory, where we performed Cr, Al, Pb and Cu trace-metal-exposure experiments with Chironomus riparius. In both situations, the genome response consisted in a statistically significant increase in somatic chromosome aberrations and decrease in BR and NOR activity to levels lower than those of larvae under standard conditions. The main chromosome aberrations consisted in inversions, amplifications, deletions and deficiencies. Deletions in the chromosome G in C. riparius transformed it into a pompon-like structure. Chromosome aberrations were not randomly distributed: they occurred mainly in sites with repetitive DNA elements that have a highly interspersed location in the C. riparius genome. Hence C. riparius is the species most likely to produce aberrations and can be used as a model for cost-effective monitoring of the early genomic response to trace metals and other stress agents.

show abstract

Section: Genotoxic Effects Of Experimental Exposure To Specific Tracementioning

confidence: 99%

Section: Genotoxic Effects Of Experimental Exposure To Specific Tracementioning

confidence: 99%

Section: Genotoxic Effects Of Experimental Exposure To Specific Tracementioning

confidence: 99%

Section: Genotoxic Effects Of Trace-metal-polluted Sediments In Naturmentioning

confidence: 99%

See 2 more Smart Citations

Chironomids (Diptera) and their salivary gland chromosomes as indicators of trace-metal genotoxicity

Michailova

Sella

Petrova

2012

Italian Journal of Zoology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Scientific literature review suggested that mutagens which affect the structural integrity of genetic material cause ectopic pairings. Numerous investigation indicated that in polyene chromosomes of different organisms including Chironomus acidophilus, Chironomus riparius and Glyptotendipes salinus exposed to different concentrations of trace metals like Al, Cr, Pb and Cu, incidences of ectopic pairings were there, as expression of abnormal interaction of different mutagens [38][39][40][41] . The incidence of ectopic pairings was dependent on the organization of the genome in the polytene chromosomes of an organism and interspersed repetitive DNA 42 .…”

Section: Resultsmentioning

confidence: 99%

Polytene Chromosome aberrations based Genotoxicity evaluation of Dichlorvos insecticide using Drosophila malenogaster

Lovleen¹,

Kashafi²

2017

IJPTR

View full text Add to dashboard Cite

: Present research work deals with evaluation of clastogenic and cytotoxic effects of Dichlorvos using larval salivary gland polytene chromosomes of Drosophila melanogaster. Exposure limit, LC 20 , was standardized by considering mortality of second instar larvae exposed to serial dilution of selected insecticide, for 24 hours. Subsequently, third instar larvae were sacrificed for temporary squash preparation of polytene chromosome, along with controls. The structural chromosomal aberration in treated stocks were incidences of ectopic pairings, paracentric inversions, chromatid breaks, chromosomal fusions, asynapses and translocations with respective value 35.7±.10.2 in treated stocks, whereas in control stocks predominant chromosomal aberrations were, paracentric inversions, chromosomal fusions and ectopic pairings with corresponding value 14.5±5.21 . Statistical analysis indicated that Dichlorvos induced significant genotoxicity in exposed larvae in comparison to control.

show abstract

Transcriptional responses, metabolic activity and mouthpart deformities in natural populations of chironomus riparius larvae exposed to environmental pollutants

Planelló

Servia

Gómez-Sande

et al. 2013

Environmental Toxicology

View full text Add to dashboard Cite

Biomarkers are an important tool in laboratory assays that link exposure or effect of specific toxicants to key molecular and cellular events, but they have not been widely used in invertebrate populations exposed to complex mixtures of environmental contaminants in their natural habitats. The present study focused on a battery of biomarkers and their comparative analysis in natural populations of the benthic larvae of Chironomus riparius (Diptera), sampled in three differentially polluted rivers (the Con, Sar, and Louro in Galicia, Spain). In our study, some parameters were identified, such as hsp70 gene activity, GST enzymatic activity, total glycogen content and mouthpart deformities, which showed significant differences among populations from the three rivers that differed in the levels and types of sedimentary contaminants analyzed (metals, organic-chlorine pesticides, alkylphenols, pharmaceutical, and personal care products). In contrast to these sensitive biomarkers, other parameters showed no significant differences (hsc70 gene, EcR gene, P450 gene, RNA:DNA ratio, total protein content), and were stable even when comparing field and nonexposed laboratory populations. The hsp70 gene seems to be particularly sensitive to conditions of pollutant exposure, while its constitutive counterpart hsc70 showed invariable expression, suggesting that the hsc70/hsp70 ratio may be a potential indicator of polluted environments. Although further studies are required to understand the correlation between molecular responses and the ecological effects of pollutants on natural populations, the results provide new data about the biological responses to multiple-stressor environments. This field study adds new molecular endpoints, including gene expression, as suitable tools that, complementing other ecotoxicological parameters, may help to improve the methodologies of freshwater monitoring under the increasing burden of xenobiotics.

show abstract

Genotoxic effect of copper on salivary gland polytene chromosomes of Chironomus riparius Meigen 1804 (Diptera, Chironomidae)

Cited by 34 publications

References 25 publications

Chironomids (Diptera) and their salivary gland chromosomes as indicators of trace-metal genotoxicity

Chironomids (Diptera) and their salivary gland chromosomes as indicators of trace-metal genotoxicity

Polytene Chromosome aberrations based Genotoxicity evaluation of Dichlorvos insecticide using Drosophila malenogaster

Transcriptional responses, metabolic activity and mouthpart deformities in natural populations of chironomus riparius larvae exposed to environmental pollutants

Contact Info

Product

Resources

About