Genotoxic effect induced by hydrogen peroxide in human hepatoma cells using comet assay

Abstract

BackgroundHydrogen peroxide (H2O2) is a common reactive oxygen intermediate generated by various forms of oxidative stress.AimThe aim of this study was to investigate the DNA damage capacity of H2O2 in HepG2 cells.MethodsCells were treated with H2O2 at concentrations of 25 µM or 50 µM for 5 min, 30 min, 40 min, 1 h, or 24 h in parallel. The extent of DNA damage was assessed by the comet assay.ResultsCompared to the control, DNA damage by 25 and 50 µM H2O2 increased significantly with increasing incubation time up to 1 h, but it was not increased at 24 h.ConclusionsOur findings confirm that H2O2 is a typical DNA damage-inducing agent and thus is a good model system to study the effects of oxidative stress. DNA damage in HepG2 cells increased significantly with H2O2 concentration and time of incubation but later decreased likely due to DNA repair mechanisms and antioxidant enzymes.

The determination of possible genetic damage to women undergoing in vitro fertilization due to infertility caused by the male factor

“…There is no statistically significant difference between study groups and control groups with regard to comet tail length and comet intensity. Increased tail length is an indicator of damage on DNA (Benhusein et al, 2010). In contrast to our study, another study conducted on an unexplained infertile patient claimed that hormones used to cure infertility increase DNA damage.…”

“…Due to conflicting results, in our study, the possible genotoxic damage to women who were exposed to in vitro fertilization due to infertility with male factor was investigated with four different genotoxicity tests (CA, SCE, MN, and comet tests). Genotoxicity tests are important as indicators of possible cancer risk (Benhusein et al, 2010;Bonassi et al, 2000Bonassi et al, , 2011Hagmar et al, 1994). This study is the first study covering four genotoxicity tests, all at once, in order to detect the genotoxic effect of in vitro fertilization treatments for patients, by analyzing the cultured and isolated lymphocytes.…”

Olive (Olea europaea L.) leaf extract counteracts genotoxicity and oxidative stress of permethrin in human lymphocytes

“…The cultures without extracts and PM were studied as control -group Mitomycin C (10 -7 M) (Vijayalaxmi et al, 1996) was used as the positive control in SCE and CA assays. Likewise, ascorbic acid (10 μM) (Turkez, 2011) and hydrogen peroxide (25 μM) (Benhusein et al, 2010) were also used as the positive controls in TAC and TOS analysis, respectively.…”

The Study of Apoptosis-inducing Effects of Three Pre-apoptotic Factors by Gallic Acid, Using Simulation Analysis and the Comet Assay Technique on the Prostatic Cancer Cell Line PC3

“…Positive control PC3 cells were treated with 30 µM H2O2 according to the method described by Benhusein et al (21).…”