Genotoxic, cytotoxic, and neurotoxic responses in Anodonta cygnea after complex metal mixture treatment

Butrimavičienė, Laura; Stankevičiūtė, Milda; Kalcienė, Virginija; Jokšas, Kęstutis; Baršienė, Janina

doi:10.1007/s11356-019-04206-1

Cited by 9 publications

(2 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…AChE activity is quite commonly inhibited by chemical stressors (Bocquené and Galgani 1998 ). For instance, AChE inhibition has been demonstrated in Anodonta cygnea , a close relative to A. anatina , after acute exposure to low levels of a complex metal mixture (Butrimavičienė et al 2019 ). While performed without replication of the effluent treatments, implication of AChE inhibition was also observed in our preliminary experiment, however, overlapping with the response range in the control group.…”

Section: Discussionmentioning

confidence: 99%

Molecular biomarker responses in the freshwater mussel Anodonta anatina exposed to an industrial wastewater effluent

Ugge

Jonsson

Berglund

2021

Environ Sci Pollut Res

View full text Add to dashboard Cite

Using a selection of molecular biomarkers, we evaluated responses in freshwater mussels (Anodonta anatina) exposed to effluent from an industrial wastewater treatment facility. The aims of this work were to (1) assess biomarkers of general toxicity under sublethal exposure to an anthropogenic mixture of chemicals, represented by an arbitrary effluent, and (2) evaluate the potential of A. anatina as a bioindicator of pollution. Adult mussels (n = in total 32; 24 males and 8 females) were exposed (96 h) in the laboratory to a fixed dilution of effluent or to a control treatment of standardized freshwater. Metal concentrations were in general higher in the effluent, by an order of magnitude or more, compared to the control. Toxic unit estimates were used as proxies of chemical stress, and Cu, Ni, and Zn were identified as potential major contributors (Cu> Ni > Zn). Six transcriptional (cat, gst, hsp70, hsp90, mt, sod) and two biochemical (AChE, GST) biomarkers were analyzed in two tissues, gills, and digestive glands. Out of the 16 responses (eight biomarkers × two tissues), 14 effect sizes were small (within ± 28 % of control) and differences non-significant (p > 0.05). Results did however show that (1) AChE activity increased by 40% in gills of exposed mussels compared to control, (2) hsp90 expression was 100% higher in exposed female gills compared to control, and (3) three marker signals (AChE in both tissues, and hsp70 in gills) differed between sexes, independent of treatment. Results highlight a need for further investigation of molecular biomarker variability and robustness in A. anatina.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular biomarker responses in the freshwater mussel Anodonta anatina exposed to an industrial wastewater effluent

Ugge

Jonsson

Berglund

2021

Environ Sci Pollut Res

View full text Add to dashboard Cite

show abstract

“…The tendency in CAT activity is consistent with earlier studies that found that certain insecticides increased CAT activity. This increase in CAT activity in the gill's aids in the pesticide's detoxification 37 . Our results come to agree with those of 38 (3).…”

Section: Table 3 Mortality Percentage Of Unio Tigridismentioning

confidence: 99%

Acute Toxicity of Chlorpyrifos on the Freshwater Bivalves (Unio Tigridis) and Effects on Bioindicators

Hanna

Shekha

2023

Baghdad Sci.J

View full text Add to dashboard Cite

A freshwater bivalve plays a crucial function in aquatic habitats as the filtered water and burrowing mussels mix the sediment, thus increasing oxygen content and making the ecosystem healthier. The aim of the study is to see how chlorpyrifos affects biochemical markers in freshwater mussel Unio tigridis. About 180 individuals per taxon and water samples were collected from the Qandil water resource on the Greater Zab River, Erbil Province, Iraq. Once arrived at the lab, the individuals were kept in aquaria with river water and an air-conditioned room Temperature: 25±2 and Light: 12h/12h and acclimatized to laboratory conditions for seven days in aged tap water. The mussel's identification molecularly and the DNA sequence of the mussel includes U. tigridis supplied gene bank accession number ON872361, ON872362, ON872363, and ON872364 nucleotide sequencing. The 96-h toxicity of chlorpyrifos pesticide in the freshwater mussel U. tigridis was investigated using various nominal concentrations, including 50, 100, 200, 300 and 400 ppm. The water quality of the river and aquaria was tested for physicochemical parameters including water temperature, the potential of hydrogen ion pH, electrical conductivity EC, and total dissolved solids TDS, dissolved oxygen, total alkalinity, total hardness, calcium ion, magnesium ion. Water quality results of aquaria revealed that most tested variables were favorable for the breeding of mussels. The mortality of the mussels was observed daily and the 96 h LC50 value for mussels was 157.99 ppm. Within the tissue of the gills, Acetylcholinesterase (AChE), Glutathione S-transferase (GST), Catalase (CAT), and Malondialdehyde (MDA) were determined. The chlorpyrifos exposures caused significant increases in GST, CAT, and MDA. The elevation of oxidative stress biomarkers was inversely related to the AChE inhibition in the examined species. In conclusion water pollution by chlorpyrifos lead to unsafe condition for aquatic taxa.

show abstract

Assessing the effects of single and binary exposures of copper and lead on Mytilus galloprovincialis: Physiological and genotoxic approaches

Crowther,

Turner,

Moore

et al. 2023

Aquatic Toxicology

View full text Add to dashboard Cite

Genotoxic, cytotoxic, and neurotoxic responses in Anodonta cygnea after complex metal mixture treatment

Cited by 9 publications

References 76 publications

Molecular biomarker responses in the freshwater mussel Anodonta anatina exposed to an industrial wastewater effluent

Molecular biomarker responses in the freshwater mussel Anodonta anatina exposed to an industrial wastewater effluent

Acute Toxicity of Chlorpyrifos on the Freshwater Bivalves (Unio Tigridis) and Effects on Bioindicators

Assessing the effects of single and binary exposures of copper and lead on Mytilus galloprovincialis: Physiological and genotoxic approaches

Contact Info

Product

Resources

About