Genotoxic, cytotoxic, and apoptotic effects of <i>Hypogymnia physodes</i> (L.) Nyl. on breast cancer cells

Arı, Ferda; Çelikler, Serap; Oran, Seyhan; Balikci, Necmiye; Öztürk, Şule; Özel, Mustafa Z.; Özyurt, Dilek; Ulukaya, Engin

doi:10.1002/tox.21809

Cited by 29 publications

(18 citation statements)

References 24 publications

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“…However, their biological activity has not been extensively studied, including that of H. physodes, physodic acid and its derivatives. To the best of our knowledge, only the cytotoxic effects of methanolic extract from H. physodes has been studied in MCF-7 and MDA-MB-231 cell lines (IC 50 ¼ 50 and 44 lg/mL, respectively) (Ari et al 2014). The cytotoxic activity of physodic acid isolated from H. physodes in FemX (IC 50 ¼ 19.52 lg/mL) and LS174 (IC 50 ¼ 17.89 lg/mL) has also been studied (Ranković et al 2014).…”

Section: Discussionmentioning

confidence: 99%

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Cytotoxic activity of physodic acid and acetone extract fromHypogymnia physodesagainst breast cancer cell lines

Studzińska-Sroka

Piotrowska

Kucińska

et al. 2016

Pharmaceutical Biology

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Context: Lichens produce specific secondary metabolites with different biological activity. Objective: This study investigated the cytotoxic effects of physodic acid, in addition to the total phenolic content and cytotoxic and antioxidant activity of acetone extract from Hypogymnia physodes (L.) Nyl. (Parmeliaceae). Materials and methods: Cytotoxicity of physodic acid (0.1-100 lM) was assessed in MDA-MB-231, MCF-7 and T-47D breast cancer cell lines and a nontumorigenic MCF-10A cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, neutral red uptake and crystal violet assays during 72 h of incubation. An MTT assay was also used to assess the cytotoxic effects of the acetone extract (0.1-100 lg/mL) in the MDA-MB-231, MCF-7, T-47D breast cancer cell lines after 72 h. The total phenolic content of the acetone extract, expressed as the gallic acid equivalent, was investigated using Folin-Ciocalteu reagent. The antioxidant activity of the extract was assessed by 2,2-diphenyl-1-picrylhydrazyl and ferric-reducing antioxidant power assays. Results: The cytotoxic activity of physodic acid appeared to be strong in the tumorigenic cell lines (IC 50 46.0-93.9 lM). The compound was inactive against the nontumorigenic MCF-10A cell line (IC 50 >100 lM). The acetone extract showed cytotoxicity in the breast cancer cell lines (IC 50 46.2-110.4 lg/mL).

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Section: Discussionmentioning

confidence: 99%

“…Previous studies examined the effects of physodic acid and acetone extract in other cancer cell lines (Pavlović et al 2013;Ari et al 2014;Ranković et al 2014). This is the first study to investigate their cytotoxic activity in breast cancer cell lines (MCF-7, T47D and MDA-MB-231).…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic activity of physodic acid and acetone extract fromHypogymnia physodesagainst breast cancer cell lines

Studzińska-Sroka

Piotrowska

Kucińska

et al. 2016

Pharmaceutical Biology

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“…36 Moreover, the methanol extracts of lichen Hypogymnia physodes displayed anti-growth effect against the human breast cancer cell lines MCF-7 and MDA-MB-231 at relatively low concentrations and genotoxic activity against cultured human lymphocytes at higher concentrations. 76 The major lichen compounds physodalic, physodic and 3-hydroxy-physodic acids from H. physodes diethyl ether extract decreased the frequency of micronucleus (at 1 mg mL À1 ) in peripheral human lymphocytes. 77 Lichen extracts from Pseudevernia furfuracea and Parmelia pulla presented remarkable antigenotoxic efficacy in peripheral lymphocytes reverting the increase of sister-chromatid exchange and micronucleus rates caused by colloidal bismuth subcitrate.…”

Section: Cytotoxic and Antitumor Activitiesmentioning

confidence: 99%

Parmeliaceae family: phytochemistry, pharmacological potential and phylogenetic features

et al. 2014

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Parmeliaceae is the largest family of lichenized fungi comprising about 2700 species distributed in about 80 genera. A wide array of secondary metabolites including depsides, depsidones, aliphatic acids, triterpenes, anthraquinones, secalonic acids, pulvinic acid derivatives and xanthones have been identified within this family. Many of these bioactive compounds such as usnic acid and atranorin are exclusively found in this family, being of great relevance for the systematics and phylogeny. Moreover, these compounds exert different biological roles including antimicrobial, antioxidant, cytotoxic, anti-inflammatory, analgesic and enzyme inhibitory actions. The current review focuses on the phytochemistry and main phylogenetic aspects of Parmeliaceae, and it discusses the pharmacological findings and mechanisms of action of their extracts and isolated compounds. The present work actually collects information related to more than 65 lichen species (from 21 different genuses and heterogeneous distribution) and 75 isolated metabolites. As reported, the best studied genus from a pharmacological point of view is Parmelia, followed by Usnea. At the species level, Cetraria islandica and Evernia prunastri attract remarkable interest in their bioactive activities and metabolite content. The major pharmacological activities investigated on extracts and compounds from Parmeliaceae spp. have been antimicrobial, anticancer and antioxidant potentials; and regarding isolated compounds, the aforementioned usnic acid and atranorin are among the most studied metabolites. Reviewed data suggest that some of these lichens and natural compounds are worthy of further investigation.

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“…In our previous study, our findings demonstrated that Hypogymnia physodes extract had an anti-growth effect at relatively lower concentrations, while relatively higher concentrations were required for genotoxic activity (Ari et al 2012). Koparal et al (2006) investigated the cytotoxic and genotoxic activities of (?…”

Section: Introductionmentioning

confidence: 99%

Promising anticancer activity of a lichen, Parmelia sulcata Taylor, against breast cancer cell lines and genotoxic effect on human lymphocytes

et al. 2014

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Plants are still to be explored for new anticancer compounds because overall success in cancer treatment is still not satisfactory. As a new possible source for such compounds, the lichens are recently taking a great attention. We, therefore, explored both the genotoxic and anti-growth properties of lichen species Parmelia sulcata Taylor. The chemical composition of P. sulcata was analyzed with comprehensive gas chromatography-time of flight mass spectrometry. Anti-growth effect was tested in human breast cancer cell lines (MCF-7 and MDA-MB-231) by the MTT and ATP viability assays, while the genotoxic activity was studied by assays for micronucleus, chromosomal aberration and DNA fragmentation in human lymphocytes culture. Cell death modes (apoptosis/necrosis) were morphologically assessed. P. sulcata inhibited the growth in a dose-dependent manner up to a dose of 100 lg/ml and induced caspase-independent apoptosis. It also showed genotoxic activity at doses ([125 lg/ml) higher than that required for apoptosis. These results suggest that P. sulcata may induce caspase-independent apoptotic cell death at lower doses, while it may be genotoxic at relatively higher doses.

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Genotoxic, cytotoxic, and apoptotic effects of Hypogymnia physodes (L.) Nyl. on breast cancer cells

Cited by 29 publications

References 24 publications

Cytotoxic activity of physodic acid and acetone extract fromHypogymnia physodesagainst breast cancer cell lines

Cytotoxic activity of physodic acid and acetone extract fromHypogymnia physodesagainst breast cancer cell lines

Parmeliaceae family: phytochemistry, pharmacological potential and phylogenetic features

Promising anticancer activity of a lichen, Parmelia sulcata Taylor, against breast cancer cell lines and genotoxic effect on human lymphocytes

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