Genotoxic consequences associated with oxidative damage in testis of mice subjected to iron intoxication

Doreswamy, Kruthika; Muralidhara, .

doi:10.1016/j.tox.2004.07.010

Cited by 33 publications

(21 citation statements)

References 46 publications

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“…Importantly, compounds that induce oxidative stress in the male germ line such as iron dextran have also been shown to generate positive results in dominant lethal assays (Doreswamy and Muralidhara, 2005). The importance of oxidative stress in the aetiology of the dominant lethal effect is also emphasized by the fact that polyphenolic antioxidants can reverse the dominant lethal impact of benzo [a]pyrene (Shukla and Taneja, 2001).…”

Section: Implications For Future Generations' Healthmentioning

confidence: 99%

The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment

Lewis

Aitken

Conner

et al. 2013

Reproductive BioMedicine Online

245

182

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Lewis, S.E.M. et al. (2013). The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment. Astract:Sperm DNA damage is a useful biomarker for male infertility diagnosis and prediction of assisted reproduction outcomes. It is associated with reduced fertilization rates, embryo quality and pregnancy rates, and higher rates of spontaneous miscarriage and childhood diseases. This review provides a synopsis of the most recent studies from each of the authors, all of whom have major track records in the field of sperm DNA damage in the clinical setting. It explores current laboratory tests and the accumulating body of knowledge concerning the relationship between sperm DNA damage and clinical outcomes. The paper proceeds to discuss the strengths, weaknesses and clinical applicability of current sperm DNA tests. Next, the biological significance of DNA damage in the male germ line is considered. Finally, as sperm DNA damage is often the result of oxidative stress in the male reproductive tract, the potential contribution of antioxidant therapy in the clinical management of this condition is discussed. DNA damage in human spermatozoa is an important attribute of semen quality. It should be part of the clinical work up and properly controlled trials addressing the effectiveness of antioxidant therapy should be undertaken as a matter of urgency. IntroductionMale factor infertility is implicated in more than 40% of couples presenting for assisted reproduction treatment. Conventional semen analysis continues to be the only routine test to diagnose this condition even though it is known that such descriptive assessments cannot discriminate between the spermatozoa of fertile and infertile men (Guzick et al., 2001). The shifting values for normality (all 'normal' values now lower) in the fifth edition of the WHO manual (World Health Organization, 2010) compared with the previous WHO editions may result in even less men being classified as infertile (Murray et al., 2012).

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Section: Implications For Future Generations' Healthmentioning

confidence: 99%

The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment

Lewis

Aitken

Conner

et al. 2013

Reproductive BioMedicine Online

245

182

View full text Add to dashboard Cite

show abstract

“…34 Additional transition metals such as iron also induce lipid peroxidation, protein carbonyl expression and lipid soluble antioxidant depletion in testicular tissue with the consequent disruption of spermatogenesis. 114,115 Significantly, iron intoxication of male mice also induces a dominant lethal effect characterized by high levels of embryonic loss in females mated to iron-exposed males. In this situation, the oxidative stress induced in the testes by acute iron overload must have so damaged the DNA in the spermatozoa that the resulting embryos were non-viable.…”

Section: Disruption Of the Antioxidant Status Of The Testesmentioning

confidence: 99%

Antioxidant Systems and Oxidative Stress in the Testes

Aitken

Roman

2009

Advances in Experimental Medicine and Biology

159

105

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“…In the group administered the highest dose, spermatogenesis was markedly lower and concentrations of antioxidants (alpha-tocopherol, ubiquinol-9, and ubiquinol-10) in the testes were inversely correlated with oxidation. Oxidative protein products viz protein thiols and protein-associated carbonyls were higher in rats exposed to the high iron dose (Doreswamy and Muralidhara, 2005). Supplementation with alpha-tocopherol partially mitigated the oxidative damage caused by chronic iron intoxication.…”

Section: Sperm and Fertilitymentioning

confidence: 91%

“…These authors cited reports in the literature that human males with -thalassaemia major have decreased pituitary function from Fe overload, and serum ferritin is highly correlated with the presence of hypogonadism. Doreswamy and Muralidhara (2005) administered acute sub-lethal doses of iron dextran to adult mice (50, 100 and 200 mg per kilogram of body weight). Multiple doses induced increase in lipid peroxidation in mitochondrial and microsomal fractions.…”

Section: Sperm and Fertilitymentioning

confidence: 99%