Genotoxic biomonitoring of tobacco farmers: Biomarkers of exposure, of early biological effects and of susceptibility

Silva, Fernanda Rabaioli da; Silva, Juliana da; Allgayer, Mariângela da Costa; Simon, C; Dias, Johnny Ferraz; Santos, Carla Eliete Iochims dos; Salvador, Mirian; Branco, Cátia Santos; Schneider, Natália; Kahl, Vivian Francília Silva; Rohr, Paula; Kvitko, Kátia

doi:10.1016/j.jhazmat.2012.04.074

Cited by 45 publications

(35 citation statements)

References 74 publications

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“…A higher number of subjects in the control group ingested alcohol, consuming once a week. However, this variable, as was the case with age and smoking, did not significantly influence the rate of DNA damage, in line with previous studies (Castillo-Cadena et al 2006;Singh et al 2011;Da Silva et al 2012).…”

Section: Discussionsupporting

confidence: 90%

“…In this sense, negative results for the MN test and positive results for the comet assay were also observed in one study of farmers exposed to pesticides also reported in this study, such as diuron, glyphosate, mancozeb, and paraquat (Remor et al 2009). However, positive results for both tests in farmers have also been reported (Da Silva et al 2012;Benedetti et al 2013), with one study including pesticides in common with this study, such as captan, glyphosate, mancozeb, and paraquat, but with greater exposure time for one of these (Da Silva et al 2012). In the other study, 80 % of workers did not use PPE during the preparation and application of pesticides (Benedetti et al 2013).…”

Section: Discussionmentioning

confidence: 58%

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Assessment of DNA damage in floriculturists in southern Brazil

Wilhelm

Calsing

Silva

2014

Environ Sci Pollut Res

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The aim of this study was to assess possible genotoxic effects on floriculturists in a region of the state of Rio Grande do Sul, in the south of Brazil, using the micronucleus test (MN) and comet assay. Thirty-seven floriculturists and 37 individuals not exposed to pesticides participated in the study. The micronucleus test was performed with epithelial cells of the oral mucosa. In the microscopic analysis, 2000 cells were evaluated per subject, verifying the frequency of MN and the frequency of other nuclear abnormalities (nuclear buds, binucleated cells, and karyorrhexis). For the comet assay in the peripheral blood lymphocytes, 100 cells were classified in five classes, according to the migration of DNA fragments, thereby generating the frequency of damaged cells and the damage index. There was no difference between the exposed and control groups in the frequencies of MN and other nuclear abnormalities in the epithelial cells of the oral mucosa. However, the comet assay showed that both the frequency of DNA damaged cells and the damage index were significantly greater in the exposed group. The results therefore indicate that floriculturists are exposed to mixtures of pesticides with genotoxic potential.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 58%

Assessment of DNA damage in floriculturists in southern Brazil

Wilhelm

Calsing

Silva

2014

Environ Sci Pollut Res

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“…However, in comparison of the frequency of cells with MN, the difference was not set, although the mean of the SG is higher than the one for the CG. These findings agree in parts with the results of other researchers who found significant differences in all types of abnormal cells when comparing the exposed group with the CG 3,29,37 . The majority of the studies have only analyzed the frequency of cells with MN, unlike this study.…”

Section: Resultssupporting

confidence: 92%

“…These findings corroborate the results of other authors 28 who, when studying genotoxic effects on tobacco growers through the comet test also showed that the treatment group presented a significant increase of DNA damage if compared to the CG. Da Silva et al 29 also observed that the DNA damage by comet assay in tobacco growers had increased three times when it was compared to the unexposed group.…”

Section: Discussionmentioning

confidence: 91%

Sistema olivococlear medial e genotoxicidade em escolares de região fumicultora

et al. 2014

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“…A study from our group observed shortening telomeres in individuals occupationally chronically exposed to low doses of pesticides at tobacco farms [33], in addition to various kinds of DNA damages already found in those individuals [81][82][83][84], corroborated by several other studies [85,86]. Pesticides are known for inducing oxidative stress [33,82,87,88], and although not all action mechanism of these chemicals are clear, induction of oxidative stress and of ROS seems to be involved.…”

Section: Occupational and Environmental Exposuressupporting

confidence: 60%

Telomere Length and Its Relation to Human Health

Kahl¹,

Silva²

2016

Telomere - A Complex End of a Chromosome

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Telomeres are complex nucleotide sequences that cap the end of chromosomes from degradation, unwanted recombination-fusion, inappropriate activation of DNA damage response and play a critical role in cell division and chromosome stability. There is growing evidence that telomere stability can be affected by occupational and environmental exposure, as some of these factors have been correlated with increase in inflammation, oxidative stress, DNA damage, chromosome aberration, and epigenetic alterations. Both extremely short and long telomeres have been associated with neurodegenerative, cardiovascular diseases (CVD) and cancer risk. Occupational and environmental exposure to several synthetic and natural chemicals has been found to be associated with changes in telomere length, although the molecular mechanism is not fully understood. Telomeric DNA is relatively less capable of repair, resulting in accelerated shortening during the cell cycle and replicative senescence. It is recognized that diet plays an important role in telomere maintenance. Prevention of exposure to environmental and occupational hazards as well as psychological stressors can reduce the risk of telomere instability. This review provides a broad evaluation of the associated mechanism between human health and environmental and occupational exposure with telomere length, including recent findings and future perspectives.

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Genotoxic biomonitoring of tobacco farmers: Biomarkers of exposure, of early biological effects and of susceptibility

Cited by 45 publications

References 74 publications

Assessment of DNA damage in floriculturists in southern Brazil

Assessment of DNA damage in floriculturists in southern Brazil

Sistema olivococlear medial e genotoxicidade em escolares de região fumicultora

Telomere Length and Its Relation to Human Health

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