Genotoxic assessment of selected native plants to deferentially exposed urban ecosystems

Phytoindication of environmental mutagenesis in the in-situ conditions using the pollen of selected species of native flora

“…The advantage of this material is its high sensitivity, which is conditioned by the haploid state, and the quantity required for the corresponding statistical analysis of the results. The use of microspores and pollen makes it possible to effectively detect parallel damage at all three structural levels of the genetic apparatus of the cell; the impact of short-term and long-term exposure; impacts of an acute and persistent environmental load as well as adaptation mechanisms, individual and population response in different types of environmental load (Mičieta & Murín 1996Solenská et al 2006;Mišík et al 2009;Pogányová et al 2017Pogányová et al , 2019.…”

“…Many studies have developed numerous methods for the detection of mutagenicity and genotoxicity in "in vivo" conditions based on the accepted plant tests in WHO / IPCS, which are currently commonly used in the Arabidopsis thaliana, Allium / Vicia chromosome aberration test, Tradescantia stamen hair mutation and micronucleus analysis, Sister chromatid exchange, Fluorescent in situ hybridization, Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL assay), Singlecell gel electrophoresis (comet assay), Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) technique, Real-time polymerase chain reaction (RT-PCR) technique, Flow cytometry (cf. Aksoy 2017) The use of plants as models for tests of environmental ecotoxicity and ecogenotoxicity was reported by, e.g., Paradiž & Lovka (1999), Pogányová et al (2017Pogányová et al ( , 2019, Mičieta & Murín (2018).…”