Genotoxic and mutagenic effects of zinc oxide nanoparticles and zinc chloride on tadpoles of Lithobates catesbeianus (Anura: Ranidae)

Motta, Andreya Gonçalves Costa; Amaral, Diogo Ferreira do; Benvindo‐Souza, Marcelino; Rocha, Thiago Lopes; Silva, Daniela de Melo e

doi:10.1016/j.enmm.2020.100356

Cited by 11 publications

(4 citation statements)

References 60 publications

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“…ZnONPs at 0.1, 1.0, and 10 mg/L exhibited DNA damage in tadpoles ( Lithobates cutesbeianus ) compared to the control group. It was revealed that the 10 mg/L had provoked a high incidence of MNs in erythrocytes and nucleated cells, indicating probable mutagenic and genotoxic consequences of ZnONPs for amphibians [ 57 ]. Other studies have also shown that NPs might cause mitochondria damage, DNA adducts, and DNA oxidation [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Use of DNA adduct and histopathological defects as indications for bio-persistence potency of zinc oxide nanoparticles in gastropod, Monacha cartusiana (Mǜller) after short-term exposure

Abdel-Halim¹,

Osman

Nassar

et al. 2022

Environ Anal Health Toxicol

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The cytotoxic effects of metallic nanoparticles (MNPs) might be revealed in genomic and histopathological defects. Therefore current study aimed to assess the bio-persistence and adverse effects potency of zinc oxide nanoparticles (ZnONPs) in the gastropod, Monacha cartusiana. Gastropods were exposed to 74 μg/mL for 14 d then the DNA adduct and histopathological defect profiles were assessed. The results demonstrated significant decline in the estimated genomic template stability (GTS%) in haemolymph and digestive gland ranging from 10.0 to 42.9% in treated animals compared to controls. In the treated and recovered snails, randomly amplified polymorphic (RAPD)-DNA showed the appearance and/or disappearance of DNA bands, indicating DNA damage due to the cytotoxicity of ZnONPs on gastropods. Significant defects in microvilli (MV), nucleus (N), mitochondria (M), and execratory glands (EXG) were noticed in the treated individuals with respect to controls. The remaining live animals were subjected to a recovery period (14 d, without treatment) and slight recovery profiles were reported for both measures compared to the control group. The recovery pattern was recognized in the nucleus/cytoplasm ratio with 0.186 and 0.428 in the treated and recovered groups concerning their control (0.176). The studied parameters reported herein might be reliable tools to assess accumulation and bio-persistence patterns of NPs in the organisms for short-term exposure indicating the cytotoxic and genotoxic effects. Also, gastropods may provide simple models for evaluating the ecotoxicological effects of nanomaterials.

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Section: Discussionmentioning

confidence: 99%

Use of DNA adduct and histopathological defects as indications for bio-persistence potency of zinc oxide nanoparticles in gastropod, Monacha cartusiana (Mǜller) after short-term exposure

Abdel-Halim¹,

Osman

Nassar

et al. 2022

Environ Anal Health Toxicol

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“…All the analyses were conducted by the same observer, using a ×20 lens. The nucleoids were evaluated on the basis of three parameters related to genomic damage, as used in the previous studies of Carvalho et al (2018) and Motta et al (2020): tail length, percentage of DNA in the tail (%DNA), and olive tail moment (OTM).…”

Section: Comet Assaymentioning

confidence: 99%

“…Given their biphasic life cycle, permeable skin, indirect development, and larval phase, amphibians are excellent bioindicators for the diagnosis of the health of an environment (Borges et al, 2019;Borges-Martins et al, 2007). Some species, in particular the most abundant and amply distributed forms, have been employed to understand how different environments or chemical substances affect an organism (Almeida et al, 2019;Motta et al, 2020). These species include Dendropsophus minutus, Boana albopunctata, and Physalaemus cuvieri, which are widely employed in ecotoxicological studies (Gonçalves et al, 2017a(Gonçalves et al, , 2019.…”

Section: Introductionmentioning

confidence: 99%

The Use of Multiple Biomarkers to Assess the Health of Anuran Amphibians in the Brazilian Cerrado Savanna: An Ecotoxicological Approach

Lopes

Benvindo‐Souza

Sotero

et al. 2023

Enviro Toxic and Chemistry

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Changes in the natural landscape and the indiscriminate use of pesticides can have a major impact on aquatic environments and have contributed to the worldwide decline of amphibian populations. In the present study, we sampled tadpoles of three anuran amphibians (Boana albopunctata, Physalaemus cuvieri, and Dendropsophus minutus) from ponds in six different agricultural landscapes of the Brazilian Cerrado savanna and evaluated whether and to what extent genotoxic and mutagenic damage was related to land use (the amount of forest and agricultural remnants, and related physicochemical factors) and the presence of pesticides in the water of the study ponds. We also evaluated the hepatotoxicity in P. cuvieri, which was the most abundant species at five of the six sampling points. Clomazone and Atrazine were the most common pesticides found in the ponds. The B. albopunctata and P. cuvieri tadpoles presented similar patterns of DNA damage among the sampling points. The least DNA damage was found in the D. minutus tadpoles, although this species was present in only one of the study ponds. More binucleated and anucleated cells were observed in B. albopunctata, but there was no significant variation among species in terms of the number of micronuclei or other erythrocytic nuclear abnormalities. Land use and physicochemical factors did not explain the variation in the DNA damage observed in the three anurans. The hepatotoxicity analyses of P. cuvieri revealed the presence of a series of alterations, including the enlargement of the sinusoids, vacuolization of the hepatocytes, the infiltration of inflammatory cells, hepatic steatosis, and dilation of the blood vessels. The interaction between physicochemical factors and the biomarkers analyzed in this study is complex. In particular, it will be important to better elucidate which factors are contributing, either directly or indirectly, to the decline of anuran amphibian populations, especially in threatened biomes, such the the Brazilian Cerrado. In this case, we would encourage further in situ studies that assess the ecotoxicology of the landscape, together with the systematic monitoring of aquatic environments, in order to guarantee the long‐term integrity of amphibian populations, and those of other organisms that play an essential functional role in the ecosystem.

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“…A single researcher analyzed all the slides under a 20x lens. In the analysis of the nucleoids, we evaluated three parameters of genomic damage, following Motta et al (2020), that is, tail length, the percentage of DNA in the tail (% DNA), and the olive tail moment (Olive).…”

Section: Comet Assaymentioning

confidence: 99%

Impacts of nickel mining on the DNA and hematological parameters of two species of bat in central Brazil

Sotero

Benvindo‐Souza

Lopes

et al. 2022

Preprint

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Exposure to heavy metals in mining zones is a significant threat, which can affect ecosystem services and contribute to the decline of wild bat populations. The present study investigated the impacts caused by mining on two bat species in central Brazil, the nectarivorous Glossophaga soricina and the frugivorous Carollia perspicillata. The bats were collected from a nickel miningzone (treatment) and protected area (control). The leukocyte profile of each species was compiled and genotoxicity (comet assay) and mutagenicity (micronucleus test) were determined using the appropriate procedures. Glossophaga soricina presented significantly higher frequencies of neutrophils and lymphocytes in the mining zone in comparison with the protected area, whereas C. perspicillata presented higher frequencies of lymphocytes in the mining zone, but significantly lower frequencies of monocytes. Concomitantly, G. soricina also presented a higher frequency of DNA damage, although no variation was found in this parameter in C. perspicillata when comparing environments. We also found no significant between populations in terms of the frequency of micronuclei and other nuclear abnormalities. Overall, the results of the study indicate that bats are susceptible to immunological disorders and DNA damage in mining zones, with the nectarivorous G. soricina appearing to be relatively more susceptible, and thus a potentially effective bioindicator of the impact of contamination in these environments.

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Genotoxic and mutagenic effects of zinc oxide nanoparticles and zinc chloride on tadpoles of Lithobates catesbeianus (Anura: Ranidae)

Cited by 11 publications

References 60 publications

Use of DNA adduct and histopathological defects as indications for bio-persistence potency of zinc oxide nanoparticles in gastropod, Monacha cartusiana (Mǜller) after short-term exposure

Use of DNA adduct and histopathological defects as indications for bio-persistence potency of zinc oxide nanoparticles in gastropod, Monacha cartusiana (Mǜller) after short-term exposure

The Use of Multiple Biomarkers to Assess the Health of Anuran Amphibians in the Brazilian Cerrado Savanna: An Ecotoxicological Approach

Impacts of nickel mining on the DNA and hematological parameters of two species of bat in central Brazil

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