Genotoxic and mutagenic assessment of spinosad using bioassays with Tradescantia pallida and Drosophila melanogaster

Mendonça, Tarcísio Paiva; Aquino, Jéssica Davi de; Silva, Weverson Junio da; Mendes, Daniele Ruela; Campos, Carlos Fernando; Vieira, Jéssica Soares; Barbosa, Nathalya Pereira; Naves, Maria Paula Carvalho; Júnior, Edimar Olegário de Campos; Rezende, Alexandre Azenha Alves de; Spanó, Mário Antônio; Bonetti, Ana Maria; Santos, Vanessa Santana Vieira; Pereira, Boscolli Barbosa; Morais, Cássio Resende de

doi:10.1016/j.chemosphere.2019.01.182

Cited by 10 publications

(4 citation statements)

References 47 publications

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“…This is despite evidence that the toxicity of spinosad to honeybees may be similar to that of imidacloprid (Challa et al, 2019;Christen et al, 2019). The mechanism of action and resistance to spinosad is being studied using whole genome sequencing of different D. melanogaster and D. suzukii strains that show different resistance levels, the polymorphisms associated with resistance are then edited into the genome using CRISPR mediated mutagenesis, with mutations in the α6 nAChR subunit being found to mediate resistance to spinosad (Zimmer et al, 2016;Mishra et al, 2018;Mendonca et al, 2019). This makes understanding the full suite of sub-lethal effects and the modes of action of neonicotinoids vital, as well as developing models for rapid identification of such effects caused by current and future pesticides.…”

Section: The Eu Ban Of Neonicotinoids and Their Continued Global Usementioning

confidence: 99%

“…Whilst we know that only some nAChR subtypes are susceptible to neonicotinoids (Moffat et al, 2016), the composition of these subtypes has not been fully determined. However, exploration of neonicotinoid resistance using D. melanogaster as a model has identified some nAChR subunits which appear to be involved in susceptibility, such as Dα1, Dβ1, and Dβ2 (Perry et al, 2012;Homem et al, 2020), in contrast α6 nAChR subunit is important for spinosad's mode of action and resistance (Zimmer et al, 2016;Mishra et al, 2018;Mendonca et al, 2019).…”

Section: Genomementioning

confidence: 99%

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The Power of Drosophila melanogaster for Modeling Neonicotinoid Effects on Pollinators and Identifying Novel Mechanisms

2021

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Neonicotinoids are the most widely used insecticides in the world and are implicated in the widespread population declines of insects including pollinators. Neonicotinoids target nicotinic acetylcholine receptors which are expressed throughout the insect central nervous system, causing a wide range of sub-lethal effects on non-target insects. Here, we review the potential of the fruit fly Drosophila melanogaster to model the sub-lethal effects of neonicotinoids on pollinators, by utilizing its well-established assays that allow rapid identification and mechanistic characterization of these effects. We compare studies on the effects of neonicotinoids on lethality, reproduction, locomotion, immunity, learning, circadian rhythms and sleep in D. melanogaster and a range of pollinators. We also highlight how the genetic tools available in D. melanogaster, such as GAL4/UAS targeted transgene expression system combined with RNAi lines to any gene in the genome including the different nicotinic acetylcholine receptor subunit genes, are set to elucidate the mechanisms that underlie the sub-lethal effects of these common pesticides. We argue that studying pollinators and D. melanogaster in tandem allows rapid elucidation of mechanisms of action, which translate well from D. melanogaster to pollinators. We focus on the recent identification of novel and important sublethal effects of neonicotinoids on circadian rhythms and sleep. The comparison of effects between D. melanogaster and pollinators and the use of genetic tools to identify mechanisms make a powerful partnership for the future discovery and testing of more specific insecticides.

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Section: The Eu Ban Of Neonicotinoids and Their Continued Global Usementioning

confidence: 99%

Section: Genomementioning

confidence: 99%

The Power of Drosophila melanogaster for Modeling Neonicotinoid Effects on Pollinators and Identifying Novel Mechanisms

2021

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“…It is structurally and chemically related to spinosyn produced by Saccharopolyspora spinosa [ 2 , 3 ]. Butenyl-spinosyn has excellent activity against various insects, such as Lepidoptera, Diptera, and Coleoptera, but has little or no effect on a broad range of nontarget insects and mammals, making it one of the most promising biological pesticides [ 4 , 5 ]. At present, the butenyl-spinosyn biosynthetic gene cluster ( bus cluster) and its biosynthetic pathway have been elaborated [ 2 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

A TetR family transcriptional regulator, SP_2854 can affect the butenyl-spinosyn biosynthesis by regulating glucose metabolism in Saccharopolyspora pogona

et al. 2022

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Background Butenyl-spinosyn produced by Saccharopolyspora pogona exhibits strong insecticidal activity and a broad pesticidal spectrum. Currently, important functional genes involve in butenyl-spinosyn biosynthesis remain unknown, which leads to difficulty in efficiently understanding its regulatory mechanism, and improving its production by metabolic engineering. Results Here, we identified a TetR family transcriptional regulator, SP_2854, that can positively regulate butenyl-spinosyn biosynthesis and affect strain growth, glucose consumption, and mycelial morphology in S. pogona. Using targeted metabolomic analyses, we found that SP_2854 overexpression enhanced glucose metabolism, while SP_2854 deletion had the opposite effect. To decipher the overproduction mechanism in detail, comparative proteomic analysis was carried out in the SP-2854 overexpressing mutant and the original strain, and we found that SP_2854 overexpression promoted the expression of proteins involved in glucose metabolism. Conclusion Our findings suggest that SP_2854 can affect strain growth and development and butenyl-spinosyn biosynthesis in S. pogona by controlling glucose metabolism. The strategy reported here will be valuable in paving the way for genetic engineering of regulatory elements in actinomycetes to improve important natural products production.

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“…Structurally, the spinosyn family is composed of a central macrolide ring system (aglycon) with rhamnose and forosamine sugars at the 9- and 17-positions, respectively. , Spinosad is a widely used insecticide featuring high efficacy against Diptera, Thysanoptera, and especially Lepidopteran pests with low toxicity to nontarget organisms . However, shortcomings of conventional spinosad formulations such as easy photolysis or hydrolysis , result in their repeated and excessive application, which thereby leads to increased environmental risks , as well as relatively high costs. Recently, sustained/controlled-release formulations of spinosad have shown great potential in reducing side effects of conventional formulations based on the advantages of small effective dose, prolonged release time, , and particularly specific environmental responsiveness. − …”

Section: Introductionmentioning

confidence: 99%

Evaluation of a Spinosad Controlled-Release Formulation Based on Chitosan Carrier: Insecticidal Activity against Plutella xylostella (L.) Larvae and Dissipation Behavior in Soil

Wang

et al. 2021

ACS Omega

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Controlled-release pesticide formulations using natural polymers as carriers are highly desirable owing to their good biocompatibility, biodegradability, and improved pesticide utilization. In this study, the application potential of our previously prepared spinosad/chitosan controlled-release suspension (SCCS) was evaluated through both toxicity and dissipation tests. A comparison with the spinosad suspension concentrate and the commercial spinosad emulsion in water showed that the insecticidal activity of SCCS against Plutella xylostella larvae displayed the best quick-acting performance as well as long-term efficacy of more than 20 days. The 48 h LC50 for a 20-day efficacy was calculated to be 29.36 mg/L. The dissipation behavior of spinosad in the spinosad/chitosan microparticles in soil was found to follow the first-order kinetics, with a relatively shorter half-life (2.1 days) than that observed for the unformulated spinosad (3.1 days). This work showed the positive effect of chitosan on spinosad in improving insecticidal activity and reducing environmental risks in soil, which provided useful information on the application potential of pesticide–carrier systems based on natural polymer materials in crop protection and food safety.

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Genotoxic and mutagenic assessment of spinosad using bioassays with Tradescantia pallida and Drosophila melanogaster

Cited by 10 publications

References 47 publications

The Power of Drosophila melanogaster for Modeling Neonicotinoid Effects on Pollinators and Identifying Novel Mechanisms

The Power of Drosophila melanogaster for Modeling Neonicotinoid Effects on Pollinators and Identifying Novel Mechanisms

A TetR family transcriptional regulator, SP_2854 can affect the butenyl-spinosyn biosynthesis by regulating glucose metabolism in Saccharopolyspora pogona

Evaluation of a Spinosad Controlled-Release Formulation Based on Chitosan Carrier: Insecticidal Activity against Plutella xylostella (L.) Larvae and Dissipation Behavior in Soil

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