Genotoxic and cytotoxic potential of whole plant extracts of Kalanchoe laciniata by Ames and MTT assay

Sharif, Ali; Akhtar, Muhammad Furqan; Akhtar, Bushra; Saleem, Ammara; Manan, Maria; Shabbir, Maryam; Ashraf, Muneeb; Peerzada, Sohaib; Ahmed, Shoaib; Raza, Mohsan

doi:10.17179/excli2016-748

Cited by 11 publications

(5 citation statements)

References 21 publications

(24 reference statements)

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“…The AE of K. laciniata also displayed thyroid peroxidase inhibition [ 151 ], immunomodulatory and anti-inflammatory properties [ 152 , 153 ]. The aqueous-methanol (AM) and n-hexane (NH) extracts of this species showed significant mutagenicity and cytotoxicity, and the NH extract treatment was more sensitive than others to E. coli [ 47 , 48 ]. Hydroethanolic extracts (HEE) obtained from K. laciniata leaves indicated dose-dependent cytotoxic activity against a 3T3 cell line (normal) and the 786–0 line (kidney carcinoma) (92.23% cell inhibition).…”

Section: Resultsmentioning

confidence: 99%

“…K. hybrida [120] Ethanol extract of the whole plant kalantubolide A (38) kalantubolide B (39) K. delagoensis [32] Flavonoids have been identified in aqueous, hydroalcoholic, and alcoholic extracts from the leaves of K. blossfeldiana, K. crenata; K. daigremontiana, K. delagoensis, K. fedtschenkoi, K. laciniata, K. marmorata, K. mortagei, and K. pinnata (compounds 40-78, Table 5 and Figure 4). The most common flavonoids/glycosylated flavonoids described from these species are derivatives of quercetin (40), patuletin (69)(70)(71), eupafolin (64), and kaempferol (48)(49)(50)(51)(52)(53)(54)(55)(56)(57)(58)(59)(60)(61)(62). Aerial parts; methanol extract from the stemsStems eupafolin (6-methoxyluteolin) (64) K. ceratophylla [17,126] Aerial parts eupafolin 4 -O-rhamnoside (65) K. ceratophylla [126] Ethanol extract of the wholeWhole plant 4 -methoxyherbacetin (66) K. delagoensis [32] Stems and leaves; Leaves K. pinnata [129] Ethanol leaf extractLeaves luteolin (77) K. ceratophylla; K. pinnata [121,126] Ethanol leaf extractLeaves luteolin 7-O-β-D-glucoside (78) K. pinnata [121] Recently, a comprehensive approach encompassing metabolomics and machine learning techniques was implemented [134] to investigate K. daigremontiana, K. × houghtonii, and K. delagoensis plant tissue cultures.…”

Section: Chemical Compositionmentioning

confidence: 99%

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A Review of the Popular Uses, Anatomical, Chemical, and Biological Aspects of Kalanchoe (Crassulaceae): A Genus of Plants Known as “Miracle Leaf”

et al. 2023

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Species of the genus Kalanchoe have a long history of therapeutic use in ethnomedicine linked to their remarkable healing properties. Several species have chemical and anatomical similarities, often leading to confusion when they are used in folk medicine. This review aims to provide an overview and discussion of the reported traditional uses, botanical aspects, chemical constituents, and pharmacological potential of the Kalanchoe species. Published scientific materials were collected from the PubMed and SciFinder databases without restriction regarding the year of publication through April 2023. Ethnopharmacological knowledge suggests that these species have been used to treat infections, inflammation, injuries, and other disorders. Typically, all parts of the plant are used for medicinal purposes either as crude extract or juice. Botanical evaluation can clarify species differentiation and can enable correct identification and validation of the scientific data. Flavonoids are the most common classes of secondary metabolites identified from Kalanchoe species and can be correlated with some biological studies (antioxidant, anti-inflammatory, and antimicrobial potential). This review summarizes several topics related to the Kalanchoe genus, supporting future studies regarding other unexplored research areas. The need to conduct further studies to confirm the popular uses and biological activities of bioactive compounds is also highlighted.

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Section: Resultsmentioning

confidence: 99%

Section: Chemical Compositionmentioning

confidence: 99%

A Review of the Popular Uses, Anatomical, Chemical, and Biological Aspects of Kalanchoe (Crassulaceae): A Genus of Plants Known as “Miracle Leaf”

et al. 2023

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“…After the time, to the multi-well plate, 100 µL of Dimethyl sulfoxide (DMSO) was added to solubilize the formazan crystals. At 540-nm wavelength, the absorbance values were measured by using a spectrophotometer ( 19 ).…”

Section: Methodsmentioning

confidence: 99%

Assessment of anticancer properties of cumin seed (Cuminum cyminum) against bone cancer

Chandrasekaran,

Krishnan,

Chacko

et al. 2023

Front. Oncol.

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IntroductionEarly-life osteosarcoma is associated with severe morbidity and mortality, particularly affecting young children and adults. The present cancer treatment regimen is exceedingly costly, and medications like ifosfamide, doxorubicin, and cisplatin have unneeded negative effects on the body. With the introduction of hyphenated technology to create medications based on plant molecules, the application of ayurvedic medicine as a new dimension (formulation, active ingredients, and nanoparticles) in the modern period is rapidly growing. The primary source of lead compounds for the development of medications for avariety of ailments is plants and their products. Traditionally, Cuminum cyminum (cumin) has been used as medication to treat a variety of illnesses and conditions.MethodsThe cumin seed was successfully extracted with solvents Hexane, Chloroform, Methanol, Ethanol and Acetone. Following the solvent extraction, the extract residue was assayed in MG63 cells for their anti-proliferative properties. ResultsFirst, we used the [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] (MTT) assay to test the extracted residue’s cytotoxicity. The results show that hexane extract Half-maximal inhibitory concentration (IC50 86 µG/mL) effciently inhibits cells by causing programmed cell death. Furthermore, using the Acridine orange/ethidium bromide (AO/EB) staining method, the lactate dehydrogenase assay, and the reactive oxygen species assay using the Dichloro-dihydro-fluorescein diacetate (DCHFDA) staining method, we have demonstrated that the hexane extract causes apoptosis in MG63 cells. Furthermore, flow cytometry research revealed that the hexane extract stops the cell cycle in the S phase. In addition, the hexane extract limits colony formation and the migration potential as shown by the scratch wound healing assay. Furthermore, the extract from cumin seeds exhibits remarkable bactericidal properties against infections that are resistant to drugs. Gas chromatography analysis was used to quantitatively determine the hexane and methanolic extract based on the experimental data. The primary chemical components of the extract are revealed by the study, and these help the malignant cells heal. The present study finds that there is scientific validity in using cumin seeds as a novel method of anticancer therapy after undergoing both intrinsic and extrinsic research.

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“…Cytotoxicity is defined as an alteration of basic cellular functions that can result in cell damage (Arencibia et al, 2019). The MTT assay is a colorimetric methodology considered as a useful tool for the determination of the cytotoxic potential of various chemicals, drugs, environmental pollutants and plant extracts (Sharif et al, 2017). The antiproliferative activity of I. sonorae has been shown in several studies (Vega-Avila et al, 2009;Torres-Moreno et al, 2015;Quintanilla-Licea et al, 2016).…”

Section: Cytotoxicity Of I Sonorae Infusions By Mtt Assaymentioning

confidence: 99%

“…Plants have widely been used in traditional medicine to treat different diseases (Sharif et al, 2017). In fact, most of the population has used herbal products as a primary source of healthcare (Ekor, 2014).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant capacity and toxicologic potential of Ibervillea sonorae plant

et al. 2022

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The main objective of this study was to evaluate the antioxidant capacity and possible toxicological potential of the infusions prepared from Ibervillea sonorae. For this, infusions were prepared from the root of I. sonorae, using the skin or the pulp. The antioxidant capacity of the infusions was evaluated through the ABTS and DPPH tests. While, the Artemia salina model, the MTT test using Vero and MCF-7 cells, and the Ames test were used to evaluate the possible toxicological potential. The results showed that I. sonorae peels were more lethal (60-80%) than pulp (<50%). Interestingly, I. sonorae pulp showed greater toxicity on Vero cells (IC50 = 222 µg / mL) than infusions made using the skins (IC50 = 379 µg / mL). Infusions of I. sonorae, either in the form of peel or pulp, showed high mutagenicity at 1000 and 500 µg/mL. The ingestion of infusions prepared from the I. sonorae plant could cause serious damage to health. DOI: https://doi.org/10.54167/tecnociencia.v16i2.903

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Genotoxic and cytotoxic potential of whole plant extracts of Kalanchoe laciniata by Ames and MTT assay

Cited by 11 publications

References 21 publications

A Review of the Popular Uses, Anatomical, Chemical, and Biological Aspects of Kalanchoe (Crassulaceae): A Genus of Plants Known as “Miracle Leaf”

A Review of the Popular Uses, Anatomical, Chemical, and Biological Aspects of Kalanchoe (Crassulaceae): A Genus of Plants Known as “Miracle Leaf”

Assessment of anticancer properties of cumin seed (Cuminum cyminum) against bone cancer

Antioxidant capacity and toxicologic potential of Ibervillea sonorae plant

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