Genotoxic and cytotoxic effects of different types of dental cement on normal cultured human lymphocytes

Bakopoulou, Athina; Mourelatos, D.; Tsiftsoglou, Asterios S.; Giassin, Nantin Panagiota; Mioglou, E.; Garefis, P.

doi:10.1016/j.mrgentox.2008.10.011

Cited by 39 publications

(28 citation statements)

References 40 publications

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“…Various types of dental materials have been reported to possess genotoxic and cytotoxic capabilities, including alterations in chromosomal integrity, cell-cycle progression, DNA replication, and repair in normal cultured human lymphocytes, although researchers have acknowledged that their results could not be directly applied to real-world clinical settings 24. Therefore, we evaluated the genotoxic and cytotoxic effects of orthodontic treatment with various orthodontic composites on human oral mucosa cells in such a setting.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the genotoxicity and cytotoxicity in the buccal epithelial cells of patients undergoing orthodontic treatment with three light-cured bonding composites by using micronucleus testing

et al. 2014

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ObjectiveThis study evaluated the cytotoxicity and genotoxicity of fixed orthodontic treatment with three different light-cured orthodontic bonding composites by analyzing micronucleus (MN) formation in the buccal mucosa during a 6-month period.MethodsThirty healthy volunteers were selected from consecutive patients referred for orthodontic treatment. Equilibrium 2 brackets and molar tubes (Dentaurum) were bonded with three different light-cured orthodontic bonding composites-Transbond XT (3M Unitek), Kurasper F (Kuraray Europe), or GrenGloo (Ormco Corporation)- to all teeth in both arches. Exfoliated buccal epithelial cells were scraped from the middle part of the inner cheeks with sterile cement spatulas before treatment and at 1, 3, and 6 months after treatment. MNs and nuclear alterations, such as karyorrhexis (KR), karyolysis (KL), and binucleated cells (BNs), were scored under a light microscope. Repeated measure ANOVA was used to calculate statistical differences in degenerative nuclear abnormalities.ResultsMN rates did not significantly differ among different time points within the same cell type (p > 0.05). In contrast, the number of BNs in buccal epithelial cells significantly increased in all composite groups (p < 0.01, Transbond XT; p < 0.001, Kurasper F and GrenGloo). KL frequency significantly increased between the beginning and end of the study in the Kurasfer F (0.80 ± 0.79 to 1.90 ± 1.10; p < 0.05) and GrenGloo (1.30 ± 1.06 to 2.40 ± 1.08; p < 0.05) groups.ConclusionsAfter 6 months of fixed orthodontic treatment with different light-cured composites, morphological signs of cytotoxicity were observed but genotoxic effects were absent.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the genotoxicity and cytotoxicity in the buccal epithelial cells of patients undergoing orthodontic treatment with three light-cured bonding composites by using micronucleus testing

et al. 2014

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“…Results from in vitro studies indicate that different types of resin cements differ extensively in their genotoxic and cytotoxic potential and their ability to affect chromosomal integrity, cell cycle progression, and DNA replication and repair. 21,22 Although these results cannot be directly extrapolated to the clinical situation, the potential occurrence of adverse effects caused by the luting agent should be considered when making clinical decisions. Further research evaluating the potential cytotoxic, genotoxic, or carcinogenic risks of resin-based cements for two-piece implant abutments are necessary.…”

Section: Discussionmentioning

confidence: 99%

Retentive Strength of Two‐Piece CAD/CAM Zirconia Implant Abutments

Gehrke

Alius

Fischer³

et al. 2013

Clin Implant Dent Rel Res

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The use of resin-based luting agents in combination with air abrasion of titanium inserts and zirconia copings led to a stable retention of two-piece CAD/CAM abutments. The bonding stability of the investigated luting agents exceeded the general limits of fracture resistance of two-piece zirconia abutments. A notable difference between the mean retention values of the tested bond materials was shown. However, the statistical analysis revealed that this difference was not significant.

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“…Ribeiro et al (27) published the results on genotoxic evaluations of Vitrebond and Ketac Molar on Chinese hamster ovary (CHO) and mouse lymphoma (L5178Y) cell lines Galić (28) showed Vitrebond's eluate to be very cytotoxic, as the first series of dilutions of both materials caused a complete mitotic arrest in lymphocyte cultures but the authors used ten-fold lower dilutions of eluate in culture treatment than we did in the present study. Also, the eluate derived from Vitrebond specimens (5 mm in diameter and 2 mm thick) caused extensive genotoxicity after 24 h, increasing the frequencies of sister chromatid exchange and the number of chromosomal aberrations (28). Of all the observed insignificant effects on micronucleus frequency, the one caused by Fuji IX was the lowest.…”

Section: Al In Vitro Evaluation Of Glass-ionomer Cementsmentioning

confidence: 98%

Micronucleus, alkaline, and human 8-oxoguanine glycosylase 1 modified comet assays evaluation of glass-ionomer cements - in vitro

Galić¹,

Tadin

Galić

et al. 2014

Archives of Industrial Hygiene and Toxicology

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The purpose of this study was to evaluate the genotoxic potential of components leached from two conventional self-curing glass-ionomer cements (Fuji IX and Ketac Molar), and light-curing, resin modified glass-ionomer cements (Vitrebond, Fuji II LC). Evaluation was performed on human lymphocytes using alkaline and hOGG1 modified comet, and micronucleus assays. Each material, polymerised and unpolymerised, was eluted in extracellular saline (1 cm 2 mL -1 ) for 1 h, 1 day, and 5 days. Cultures were treated with eluates using final dilutions of 10 . Alkaline comet assay did not detect changes in DNA migration of treated cells regardless of the ionomer tested, polymerisation state, and elution duration. Glass ionomers failed to significantly influence micronucleus frequency. No oxidative DNA damage in treated lymphocytes was observed using hOGG1 modified comet assay. Obtained results indicate high biocompatibility of all tested materials used in the study under experimental conditions.

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Genotoxic and cytotoxic effects of different types of dental cement on normal cultured human lymphocytes

Cited by 39 publications

References 40 publications

Evaluation of the genotoxicity and cytotoxicity in the buccal epithelial cells of patients undergoing orthodontic treatment with three light-cured bonding composites by using micronucleus testing

Evaluation of the genotoxicity and cytotoxicity in the buccal epithelial cells of patients undergoing orthodontic treatment with three light-cured bonding composites by using micronucleus testing

Retentive Strength of Two‐Piece CAD/CAM Zirconia Implant Abutments

Micronucleus, alkaline, and human 8-oxoguanine glycosylase 1 modified comet assays evaluation of glass-ionomer cements - in vitro

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