Genotoxic and cytotoxic effects in the bivalve mollusks <i>Macoma balthica</i> and <i>Mytilus edulis</i> from the Baltic Sea

The Distribution of the MusselMytilusSpecies Along the Norwegian Coast

“…The large inter-individual variability associated to the low baseline frequency for this biomarker confirming the need for the scoring of a consistent number of cells in an adequate number of animals for each study point. Sampling size in most of studies conducted with mollusc species have been scoring 1000-2000 cells per animal (Izquierdo et al, 2003;Hagger et al, 2005;Bolognesi et al, , 2004Bolognesi et al, , 2006aMagni et al, 2006;Baršienė et al, , 2006bBaršienė et al, , 2008bBaršienė et al, , 2010aBaršienė et al, , 2010bKopecka et al, 2006;Nigro et al, 2006;Schiedek et al, 2006;Francioni et al, 2007;Siu et al, 2008;Koukouzika andDimitriadis, 2005, 2008) and previous reviews have sug-gested that when using fish erythrocytes at least 2000-4000 cells should be scored per animal (Udroiu et al, 2006;Bolognesi et al, 2006). Previously scorings of 5000-10 000 fish erythrocytes where used for a MN analysis (Baršienė et al, 2004).…”

“…Mussel sampling size in MN assays range from 5 to 20 mussels per site as reported in the literature (Venier and Zampieron, 1997;Bolognesi et al, 2004;Baršienė et al, 2004Baršienė et al, , 2006e, 2008aBaršienė et al, , 2008bFrancioni et al, 2007;Siu et al, 2008). Evidence suggests that a sample size of ten specimens per site is enough for the assessment of environmental genotoxicity levels and evaluation of the existence of genetic risk zones.…”

Genotoxic and Biochemical Biomarker Responses in Meretrix Casta Exposed to Environmentally Relevant Concentrations of Cadmium

“…The Micronucleus (MN) test was performed following the protocol outlined by Baršiene et al (32). A portion of the gill tissue was placed in a drop of methanol acetic acid mixture (3:1) on a clean glass slide.…”

Functions of metallothioneins and a system of antioxidant defense under the effect of Co- and Zn-containing nanocomposites on crucian carp (Carassius auratus gibelio)

“…Відповідні зміни спостерігали після забарв-лення цитологічних препаратів 5%-им розчи-ном гімзи під мікроскопом з олійною імерсією, ×1000 і виражали з розрахунку на 1000 клітин (‰) [20]. Загальна кількість проаналізованих клітин на цитологічному препараті складала не менше 2 тис.…”