Genotipificación de aislamientos clínicos de Aspergillus flavus y su relación con aislamientos ambientales de un centro oncohematológico

Refojo, Nicolás; Duarte-Escalante, Esperanza; Dignani, María Cecilia; Hevia, Alejandra; Abrantes, Rubén; Davel, Graciela; Canteros, Cristina Elena; Frías-De-León, María Guadalupe; Acosta-Altamirano, Gustavo; Zúñiga, Gerardo; Reyes-Montes, María del Roció

doi:10.1016/j.riam.2012.09.004

Cited by 4 publications

(7 citation statements)

References 45 publications

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“…1 filter paper. The mycelium DNA was extracted using a DNeasy ® Plant Mini Kit (Qiagen, Austin, TX, USA), as described by Refojo et al [16]. DNA concentration and purity (absorption ratio 260/280 = 1.8-2.0) were determined in a DS-11 spectrophotometer (DeNovix, Wilmington, DE, USA).…”

Section: Dna Extractionmentioning

confidence: 99%

“…In addition, it is important to know the molecular distribution and epidemiology of Aspergillus species obtained from clinical and environmental sources in different geographic regions of the world. To obtain information on different aspects of the disease and the etiological agents that cause it, molecular epidemiology studies were carried out using different molecular methods [ 12 , 13 , 14 , 15 , 16 ]. Among these methods is a very accurate typing method that involves a 12-mer microsatellite found in the region coding for a putative cell surface protein (CSP) (AFUA_3G08990); this method was used to type A. fumigatus isolates obtained from nosocomial outbreaks of invasive aspergillosis, and is able to demonstrate clonality and different genotypes among the isolates [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

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Identification of CSP Types and Genotypic Variability of Clinical and Environmental Isolates of Aspergillus fumigatus from Different Geographic Origins

et al. 2020

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The CSP (cell surface protein) microsatellite marker is useful for typing Aspergillus fumigatus isolates and determining relationships at the subpopulation level because it has shown high discriminatory power. In the present study, 90 A. fumigatus isolates from Mexico (MX), Argentina (AR), France (FR), and Peru (PE) were identified through a phylogenetic analysis using the benA gene fragment and were typed with the CSP microsatellite, and the types were identified using the nomenclature recommended in the literature. Genetic variability was analyzed through haplotype diversity, nucleotide diversity, polymorphic sites, and nucleotide differences between pairs of sequences. The population structure was evaluated using the Tajima’s D statistic. No new CSP types were recorded in the MX, FR, and PE isolates, while in the AR isolates, two new CSP types were identified (t25 and t26). The most common CSP types in the studied populations were t01, t02, t03, and t04A; these results are consistent with findings in other countries. In addition, the genetic diversity parameters we obtained revealed that the greatest genetic diversity was found in the MX population, followed by AR and FR. No population structure was identified among the isolates studied.

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Section: Dna Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of CSP Types and Genotypic Variability of Clinical and Environmental Isolates of Aspergillus fumigatus from Different Geographic Origins

et al. 2020

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“…Molecular identification of fungi in hospitals has been scarcely described [ 156 – 158 ]. Lo Passo et al [ 156 ] reported transmission of Trichosporon asahii by an endoscopic procedure, when isolated from an esophageal ulcer.…”

Section: Molecular Biology As a Diagnostic Tool In Haimentioning

confidence: 99%

“… The complexities of the pulmonary microbiome and its metagenomic diversity represent a great challenge with many unanswered questions remaining [ 155 ]. New procedures combining molecular biology techniques and environmental sampling of air have revealed some fungal pathogens living in the hospital surroundings [ 158 ], which may be relevant for the acquisition of respiratory HAI. …”

Section: Molecular Biology As a Diagnostic Tool In Haimentioning

confidence: 99%

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CouldHistoplasma capsulatumBe Related to Healthcare-Associated Infections?

Carreto-Binaghi

Damasceno

Pitangui

et al. 2015

BioMed Research International

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Healthcare-associated infections (HAI) are described in diverse settings. The main etiologic agents of HAI are bacteria (85%) and fungi (13%). Some factors increase the risk for HAI, particularly the use of medical devices; patients with severe cuts, wounds, and burns; stays in the intensive care unit, surgery, and hospital reconstruction works. Several fungal HAI are caused by Candida spp., usually from an endogenous source; however, cross-transmission via the hands of healthcare workers or contaminated devices can occur. Although other medically important fungi, such as Blastomyces dermatitidis, Paracoccidioides brasiliensis, and Histoplasma capsulatum, have never been considered nosocomial pathogens, there are some factors that point out the pros and cons for this possibility. Among these fungi, H. capsulatum infection has been linked to different medical devices and surgery implants. The filamentous form of H. capsulatum may be present in hospital settings, as this fungus adapts to different types of climates and has great dispersion ability. Although conventional pathogen identification techniques have never identified H. capsulatum in the hospital environment, molecular biology procedures could be useful in this setting. More research on H. capsulatum as a HAI etiologic agent is needed, since it causes a severe and often fatal disease in immunocompromised patients.

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