Genomovar assignment of <i>Pseudomonas stutzeri</i> populations inhabiting produced oil reservoirs

Zhang, Fan; She, Yuanbin; Banat, Ibrahim M.; Chai, Lujun; Huang, Liuqin; Shao-jin, YI; Wang, Zhengliang; Dong, Hailiang; Hou, Dujie

doi:10.1002/mbo3.179

Cited by 10 publications

(6 citation statements)

References 48 publications

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“…P. stutzeri was also relatively abundant at day 0, indicating that it may play an important role in degradation of NA and other compounds in the OSPW. Indeed, P. stutzeri was enriched on high molecular weight PAHs from the same sample, TPW, by Folwell et al (2016), and has also been detected in abundance in a range of hydrocarbon-contaminated environments, including a tar pit (Kim and Crowley, 2007) and oil reservoirs (Zhang et al, 2014). Therefore, the capacity to transform structurally complex diamondoid carboxylic acids as well as a range of other toxic compounds found is OSPW, makes P. stutzeri a prime candidate for bioremediation of such environments.…”

Section: Pseudomonas Stutzeri a Versatile And Abundant Member Of Thementioning

confidence: 99%

Diamondoids are not forever: microbial biotransformation of diamondoid carboxylic acids

Folwell

McGenity

Whitby

2019

Microbial Biotechnology

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Summary Oil sands process‐affected waters (OSPW) contain persistent, toxic naphthenic acids (NAs), including the abundant yet little‐studied diamondoid carboxylic acids. Therefore, we investigated the aerobic microbial biotransformation of two of the most abundant, chronically toxic and environmentally relevant diamondoid carboxylic acids: adamantane‐1‐carboxylic acid (A1CA) and 3‐ethyl adamantane carboxylic acid (3EA). We inoculated into minimal salts media with diamondoid carboxylic acids as sole carbon and energy source two samples: (i) a surface water sample (designated TPW) collected from a test pit from the Mildred Lake Settling Basin and (ii) a water sample (designated 2 m) collected at a water depth of 2 m from a tailings pond. By day 33, in TPW enrichments, 71% of A1CA and 50% of 3EA was transformed, with 50% reduction in EC20 toxicity. Similar results were found for 2 m enrichments. Biotransformation of A1CA and 3EA resulted in the production of two metabolites, tentatively identified as 2‐hydroxyadamantane‐1‐carboxylic acid and 3‐ethyladamantane‐2‐ol respectively. Accumulation of both metabolites was less than the loss of the parent compound, indicating that they would have continued to be transformed beyond 33 days and not accumulate as dead‐end metabolites. There were shifts in bacterial community composition during biotransformation, with Pseudomonas species, especially P. stutzeri, dominating enrichments irrespective of the diamondoid carboxylic acid. In conclusion, we demonstrated the microbial biotransformation of two diamondoid carboxylic acids, which has potential application for their removal and detoxification from vast OSPW that are a major environmental threat.

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Section: Pseudomonas Stutzeri a Versatile And Abundant Member Of Thementioning

confidence: 99%

Diamondoids are not forever: microbial biotransformation of diamondoid carboxylic acids

Folwell

McGenity

Whitby

2019

Microbial Biotechnology

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“…This method has previously been described as being useful for the characterization of bacterial isolates ( Adderson et al, 2008 ). Through that analysis, the isolate was confirmed to belong to the genus Pseudomonas and the species P. stutzeri ( Bennasar et al, 1996 ; Zhang et al, 2014 ). The 16S rRNA sequence was subsequently submitted to NCBI ( KF894696 ).…”

Section: Discussionmentioning

confidence: 97%

Isolation, identification, and pathological effects of beach sand bacterial extract on human skin keratinocytesin vitro

Subhan

Shahzad

Tauseef

et al. 2018

PeerJ

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BackgroundBeaches are recreational spots for people. However, beach sand contains harmful microbes that affect human health, and there are no established methods for either sampling and identifying beach-borne pathogens or managing the quality of beach sand.MethodThis study was conducted with the aim of improving human safety at beaches and augmenting the quality of the beach experience. Beach sand was used as a resource to isolate bacteria due to its distinctive features and the biodiversity of the beach sand biota. A selected bacterial isolate termed FSRS was identified as Pseudomonas stutzeri using 16S rRNA sequencing and phylogenetic analysis, and the sequence was deposited in the NCBI GenBank database under the accession number MF599548. The isolated P. stutzeri bacterium was cultured in Luria–Bertani growth medium, and a crude extract was prepared using ethyl acetate to examine the potential pathogenic effect of P. stutzeri on human skin. A human skin keratinocyte cell line (HaCaT) was used to assess cell adhesion, cell viability, and cell proliferation using a morphological analysis and a WST-1 assay.ResultThe crude P. stutzeri extract inhibited cell adhesion and decreased cell viability in HaCaT cells. We concluded that the crude extract of P. stutzeri FSRS had a strong pathological effect on human skin cells.DiscussionBeach visitors frequently get skin infections, but the exact cause of the infections is yet to be determined. The beach sand bacterium P. stutzeri may, therefore, be responsible for some of the dermatological problems experienced by people visiting the beach.

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“…Manuscript to be reviewed 1996; Zhang et al, 2014). The 16S rRNA sequence was subsequently submitted to NCBI (KF894696).…”

Section: P Stutzeri Was First Named Bymentioning

confidence: 99%

Peer Review #2 of "Isolation, identification, and pathological effects of beach sand bacterial extract on human skin keratinocytes in vitro (v0.1)"

2018

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Background. Beaches are recreational spots for people. However, beach sand contains harmful microbes that affect human health, and there are no established methods for either sampling and identifying beach-borne pathogens or managing the quality of beach sand. Method. This study was conducted with the aim of improving human safety at beaches and augmenting the quality of the beach experience. Beach sand was used as a resource to isolate bacteria due to its distinctive features and the biodiversity of the beach sand biota. A selected bacterial isolate termed FSRS was identified as Pseudomonas stutzeri using 16S rRNA sequencing and phylogenetic analysis, and the sequence was deposited in the NCBI GenBank database under the accession number MF599548. The isolated P. stutzeri bacterium was cultured in Luria-Bertani growth medium, and a crude extract was prepared using ethyl acetate to examine the potential pathogenic effect of P. stutzeri on human skin. A human skin keratinocyte cell line (HaCaT) was used to assess cell adhesion, cell viability, and cell proliferation using a morphological analysis and a WST-1 assay. Result. The crude P. stutzeri extract inhibited cell adhesion and decreased cell viability in HaCaT cells. We concluded that the crude extract of P. stutzeri FSRS had a strong pathological effect on human skin cells. Discussion. Beach visitors frequently get skin infections, but the exact cause of the infections is yet to be determined. The beach sand bacterium P. stutzeri may, therefore, be responsible for some of the dermatological problems experienced by people visiting the beach.

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Genomovar assignment of Pseudomonas stutzeri populations inhabiting produced oil reservoirs

Cited by 10 publications

References 48 publications

Diamondoids are not forever: microbial biotransformation of diamondoid carboxylic acids

Diamondoids are not forever: microbial biotransformation of diamondoid carboxylic acids

Isolation, identification, and pathological effects of beach sand bacterial extract on human skin keratinocytesin vitro

Peer Review #2 of "Isolation, identification, and pathological effects of beach sand bacterial extract on human skin keratinocytes in vitro (v0.1)"

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